Overview
This lecture explains the correct procedure for collecting, preparing, and analyzing blood samples using a syringe and a cartridge-based testing system.
Sample Collection & Preparation
- Discard air bubbles and the first two drops of blood before collecting the sample.
- For a 1 mL syringe, collect at least 0.5 mL; for a 3 mL syringe, collect at least 0.8 mL.
- Adjust the syringe to match the required blood collection volume before sampling.
- After collection, expel any air bubbles and isolate air from the sample.
- Mix the blood sample by rolling the syringe between hands and inverting it vertically to ensure thorough mixing.
Cartridge Preparation & Sample Loading
- Select the correct sample type on the device’s main screen.
- Scan the barcode on the new cartridge's foil pouch.
- If using a refrigerated cartridge, warm it for 2-5 minutes before use.
- Discard the first two drops of blood when preparing to load the sample.
- Insert the syringe fully into the fill port, then insert the cartridge into the cartridge port and press until it clicks into place.
Device Operation & Analysis
- Enter required patient information (ID, temperature, FiO2) and press OK to save.
- The machine automatically calibrates, samples, and measures the blood sample.
- After the test, results display on the screen, and the test cartridge should be removed immediately.
Key Terms & Definitions
- FiO2 — fraction of inspired oxygen, the percentage of oxygen a patient breathes.
- Cartridge — a disposable unit containing reagents for blood sample analysis.
- Mixing — rolling and inverting sample to ensure homogeneity before testing.
Action Items / Next Steps
- Practice the sample preparation and mixing procedures.
- Review the device's user manual for specific cartridge handling instructions.