36-Hour Fast Phases Overview

Nov 17, 2025

Overview

Speaker explains a 36-hour fast, outlining five phases and hour-by-hour metabolic changes affecting fat loss, hunger, hormones, and cellular repair.

Phases and Timeline

  • Five phases: Glycogen Depletion, Metabolic Switch, Deep Ketosis and Autophagy, Peak Metabolic Transformation, Reset and Recovery.
  • Claims based on personal experience, clients, and cited research across multiple institutions and journals.

Glycogen Depletion (Hours 0–12)

  • Hours 0–4: Fed state transition with elevated insulin and active digestion.
  • Body uses stored glycogen: muscles ~400–500 g; liver ~100–200 g.
  • Hours 4–8: Liver glycogenolysis begins; 10–15 g/hour depletion rate cited.
  • Growth hormone rises 2–3x within first 8 hours; pulses amplified by fasting.
  • Hours 8–12: Hunger peaks; ghrelin up 30–40% above baseline, temporary spike.
  • By hour 12: Liver glycogen 60–70% depleted; insulin sensitivity starts improving.

Metabolic Switch (Hours 12–18)

  • Hours 12–15: Shift from glucose to fat burning; “metabolic switch.”
  • Liver glycogen down to ~20–30 g; fat oxidation enzymes increase (CPT1 up).
  • Hours 15–18: Ketone production begins; blood ketones ~0.5–1 mmol.
  • Brain begins using ketones via monocarboxylate transporters; mental clarity reported.
  • Ghrelin paradox: Hunger decreases as body prioritizes stored fuel use.

Deep Ketosis and Autophagy (Hours 18–24)

  • Hours 18–21: Ketone production up 400–500% vs fed state; ~1.5–2 mmol ketones.
  • Brain energy from ketones rises to 60–70% during this window.
  • Hours 21–24: Autophagy increases 300–400%; mTOR decreases ~50% in extended fast.
  • Growth hormone surges: ~5x in men, ~14x in women; muscle preservation emphasized.
  • By hour 24: Insulin sensitivity improves 30–40%; benefits may persist up to 72 hours.

Peak Metabolic Transformation (Hours 24–30)

  • Hours 24–27: Fat oxidation peaks; cited ~160 mg/min, ~3x fed state.
  • Blood ketones reach ~2–3.5 mmol; within “nutritional ketosis,” below dangerous range.
  • Respiratory quotient drops to ~0.7–0.75, indicating predominant fat usage.
  • Hours 27–30: Autophagy markers peak; LC3-II up 300–400%, p62 down.
  • Neuronal autophagy up ~400%; potential reduction of damaged protein accumulation.
  • BDNF up 200–300%; supports neurogenesis, synaptic strength, and protection.
  • Hormonal profile: Growth hormone up 5–14x, insulin down 50–70%, norepinephrine up 40–50%, cortisol elevated for gluconeogenesis.

Reset and Recovery (Hours 30–36)

  • Hours 30–33: Peak metabolic flexibility; improved switching between fuels.
  • Mitochondrial biogenesis reported up to ~40%; increased cellular “power plants.”
  • Hours 30–36: Digestive system begins enzyme production in anticipation of food.
  • Growth hormone remains elevated; insulin sensitivity peaks; mental clarity common.
  • Ketones provide stable energy with fewer perceived spikes and crashes.

Structured Summary

PhaseHoursPrimary Fuel StateKey ProcessesHormonal/Neural ChangesKetone Levels (mmol)
Glycogen Depletion0–12Glucose to glycogenGlycogenolysis; early GH riseInsulin declines; ghrelin spikes~0
Metabolic Switch12–18Shift to fatCPT1 up; beta-oxidation startsHunger decreases; brain begins ketone use~0.5–1
Deep Ketosis & Autophagy18–24Fat predominantKetones surge; autophagy up 300–400%GH surges; mTOR down~1.5–2
Peak Transformation24–30Fat dominantMax fat oxidation; peak autophagyGH up 5–14x; NE up 40–50%~2–3.5
Reset & Recovery30–36Fat with prep for feedingMitochondrial biogenesis; enzyme prepHigh insulin sensitivity; mental claritySustained

Reported Benefits

  • Fat oxidation increased; RQ shifts toward fat utilization.
  • Muscle preservation supported by elevated growth hormone.
  • Insulin sensitivity improved; nutrients used efficiently post-fast.
  • Autophagy-driven cellular cleanup; neuronal autophagy emphasized.
  • Cognitive benefits: mental clarity and focus; BDNF increase.
  • Metabolic flexibility enhanced; potential persistence 72–96 hours post-fast.
  • Lower inflammatory markers reported across the body.

Breaking the Fast

  • First meal: 300–400 calories, small and easily digestible, nutrient-focused.
  • Suggested options include bone broth and steamed vegetables.
  • Avoid large carbohydrate load or binge eating due to high insulin sensitivity.
  • Satiety signals heightened; smaller portions likely sufficient.

Disclaimers and Audience Notes

  • Speaker: Dr. Michael Diamonds, medical doctor, scientist, pro-natural bodybuilder.
  • Claims guided by personal experience and clients; research references cited.
  • Not recommended for unhealthy individuals; cautions for specific populations implied.

Action Items

  • For those attempting: Plan a 36-hour fast with clear phases and refeed strategy.
  • Prepare small, nutrient-dense foods for breaking the fast.
  • Track hunger patterns around 12–15 hours to navigate ghrelin spikes.
  • Monitor cognitive and energy changes during ketosis window.

Decisions

  • Adopt a structured 36-hour fast protocol emphasizing careful refeeding and observation of metabolic responses.