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Fermentation Experiment with Yeast

Oct 28, 2025

Overview

This lecture demonstrates an experiment on fermentation using yeast and sugar, highlighting the production of carbon dioxide gas and ethanol.

What Is Fermentation?

  • Fermentation is the chemical breakdown of substances by microorganisms like yeast or bacteria, producing gases and heat.
  • Yeast is a type of fungus used in food processes such as bread rising.

Experiment Setup

  • Materials: several bottles, sugar, yeast, balloons, funnel, measuring utensils, warm water, gloves, goggles, apron/lab coat.
  • Each bottle receives 2ΒΌ teaspoons of yeast.
  • Sugar amounts: bottle 1 (none), bottle 2 (1 tsp), bottle 3 (2 tsp), bottle 4 (3 tsp).
  • Add 1 cup of warm water to each bottle.
  • Mix ingredients, cover with a balloon, and let sit in a warm area for 1-2 hours.

Experimental Observations & Results

  • Balloons inflate over time as gas fills them.
  • Bottles with more sugar cause larger balloon inflation.
  • Yeast consumes sugar, producing carbon dioxide (COβ‚‚) and ethanol as waste products.

Science Behind the Experiment

  • Yeast feeds on sugars and starches, metabolizing them for energy.
  • The metabolic process releases carbon dioxide gas and ethanol.
  • COβ‚‚ gas pressure inflates the balloon.
  • Reaction equation: Sugar (sucrose) β†’ Ethanol + COβ‚‚.

Key Terms & Definitions

  • Fermentation β€” Breakdown of substances by microorganisms, producing gas and heat.
  • Yeast β€” A fungus used in fermentation processes; microscopic and widely used in baking.
  • Carbon Dioxide (COβ‚‚) β€” Gas produced during fermentation.
  • Ethanol β€” Alcohol produced as a fermentation byproduct.
  • Metabolism β€” Chemical process in living organisms to convert food to energy.

Action Items / Next Steps

  • If replicating the experiment at home, always get parental permission before sharing photos or videos.
  • Wear appropriate safety gear (gloves, goggles, apron/lab coat) during the experiment.