Pipetting Techniques

Overview

• Two basic pipetting techniques:
  1. Forward Pipetting
  2. Reverse Pipetting

Forward Pipetting Technique

• Common technique used in many laboratories.

Reverse Pipetting Technique

• Description:
  • The operation button is pressed to the second stop before aspirating liquid.
  • This action aspirates an excess (access) volume of liquid.
• Dispensing Process:
  • The operation button is pressed only to the first stop to dispense the target volume.
  • The access volume is either returned to the source or discarded.

Benefits of Reverse Pipetting

• Improved Accuracy:
  • Particularly beneficial for dispensing viscous, volatile, and foaming liquids.
• Enhanced Repeatability:
  • Small volumes are dispensed more accurately.
• Reduces Errors:
  • Liquid sticking to the tip or evaporating during the operation does not affect the target volume.

Conclusion

• Knowing and utilizing both pipetting techniques can enhance precision in laboratory settings.