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What happens during the homogenization process in cell fractionation?
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Cells are broken apart and plasma membranes are disrupted, allowing internal contents to mix into a homogenate.
How does differential centrifugation aid in the study of ribosomes?
By allowing for the isolation of ribosomes for examination of their role in protein synthesis.
What is the significance of isolating mitochondria through differential centrifugation?
Isolation allows researchers to investigate mitochondrial functions such as energy production in detail.
What is the result of centrifuging the supernatant at 80,000 G's?
It produces a pellet rich in microsomes, which are pieces of plasma membrane and internal membranes.
Explain the benefit of fractionating cells in the study of cellular component functions.
Fractionation allows for the analysis of specific organelles without interference from others, providing clarity in functional studies.
What does microsome-rich pellet obtained at 80,000 G's centrifugation consist of?
It consists of pieces of plasma membrane and internal membranes.
What components form the pellet when supernatant is centrifuged at 20,000 G's?
A pellet rich in mitochondria is formed.
Why is the technique of differential centrifugation important in cellular biology?
It allows researchers to isolate and analyze specific cellular organelles, aiding in understanding their functions and roles.
In the process of cell fractionation, what is the purpose of the differential speeds used in centrifugation?
Different speeds are used to separate cell components based on size and density, with each speed targeting specific organelles.
At what speed is the homogenate first centrifuged, and what does the resulting pellet contain?
The homogenate is first centrifuged at 1,000 G's, producing a pellet rich in nuclei and cell debris.
What is the primary purpose of cell fractionation?
To study the different parts of a cell by separating them based on their size and density.
How does the method of differential centrifugation facilitate detailed studies of subcellular structures?
By isolating specific organelles, it allows researchers to focus on their unique structures and functions without distractions.
What is obtained when supernatant is centrifuged at 150,000 G's and why is this step significant?
A pellet rich in ribosomes is obtained, allowing for detailed studies of protein synthesis machinery.
What cellular components are targeted during the 80,000 G's centrifugation step, and what do they represent?
Microsomes are targeted, representing fragmented pieces of the plasma membrane and internal membranes.
Describe the composition of the supernatant and pellet after a centrifugation at 1,000 G's.
The pellet consists of nuclei and cell debris, while the supernatant contains smaller cellular components.
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