okay today I'm gonna show you some different transfer techniques that we use in microbiology so to start with some cultures will be ground on a petri dish it's so hard auger is kind of got gel type consistency so it's a hard medium that we will grow sometimes cultures on sometimes we will grow them on what we call a slant that's where you've got the auger in the auger you can make different types of nutrients add it to it and so this would be a slight you can inoculate that and sometimes they will be grown in a liquid broth as you can see in the test tube but if usually when it's in liquid bacteria will turn it cloudy slightly cloudy I don't know if you can tell that that has some particles in it that's the bacteria though this has grown for about 24 hours and so today what we're going to do is show you how we transfer from one meeting to another now we also have what we call our inoculating loops and needles inoculating needle is just a straight piece here we don't use that quite this often the thing we use most often will be the inoculating loop as you can tell it has some literally a loop at the end and we use this to touch the bacteria and one medium and transfer it to another now we're always going to be working with the flame and walking near that flame general rule of thumb is that within about six no more than eight inches around that flame is considered your sterile area so you whenever you open a petri dish open the two you want to be within that that range so that you don't get contamination from the air or some colleague walking by etc so you always want to be working on that it that close area now for today what I'm going to do is we have a stop culture it is growing on this petri dish and I'm going to show you how to transfer it to another petri dish so we're going from solid meeting to solid medium and then also going to show you also to another Sala meeting but that's going to be this slant oftentimes when you're working with testes you may want before you get started it's loosen the lid just so be easier when you're ready to go for what you need to do to start off with is take your inoculating loop in this case we are using the loop you need to stick it into the flame this is an incineration it is a way of sterilizing that loop you're literally burning everything off you want to get it where it's glowing nice and red that kills everything that has been on this loop now you do not want to immediately stick it on the plate or you're going to kill everything because it's hot and if you go if you're impatient and you go a little too soon you're gonna hear a little sizzle that means you just killed everything remember bacteria very small do you have to go into the plate know if you have enough that you can see it on the end of the loop that is too much so what we're going to do here ideally you want to take just one colony now we're just going to show you how to transfer you so you just take a little bit you don't open that land and fling it around keep the lid as closed as possible we are not going for an isolated colony so all we are going to do is just go back and forth with the loop the bacteria that are on the edge of this loop will be transferred to that plate that is now inoculated flame your loop again at the end to kill anything that's on it you'll notice I flipped that petri dish upside down we always incubate petri dishes upside down the inverted position the reason for that is because you can see on this plate that has been stored we store it in the fridge after 24 hours you get condensation on it when you are trying to grow cultures that condensation will mess up try and get individual colonies so we always invert them so now I'm going to take another sample from the plate now I'm going to go to the test to unscrew the lid you're going to pass the lip of the test tube through the flame that is to reduce potential contamination that may hip in on it stick the loop in to the - all the way down towards the bottom and then you just kind of zigzag up flame the loop very flame that the tube excuse me now flame the loop again so the test tube you notice that flamed it before and after opening it once again trying to reduce any potential contamination that that could occur you flame your loop between every process now you're going to want to label everything some people will label with tape some people will label with a sharpie I recommend if you're labeling with this sharpie on the page you just label it on the bottom just in case something would happen in the lid would come off you don't know what's growing on that plate so in this case I'm labeling it with the initials of the organism we use to ratio marcescens I will put today's date on there I'm also going to put my initials on here so if anybody has a question they know who inoculated it the initials up here in a stand for the media so we know that it is a nutrient augur just about anything will grow on that sometimes you may want to label things with a piece of tape whichever you choose to do it does not matter just as long as you do label everything these will be incubated at room temp oh sometimes you incubate at 37 degrees sometimes at room temperature it just depends on what you are doing and for this particular exercise I do want to go at room temperature and we will go for take four hours sometimes you may need to go for 48 hours once again that depends on what tests you are using so on my label once again I'm going to put the organism I'm going to put the date and I'm going to put my initials on here and we'll just place that on the - once again that way there is no question about what is on your sample so that is how you would go from us solid medium to a solid we showed you both with the plate and with the slant as you can see we have growth here bacteria oftentimes will look kind of a beige depending on the species maybe a yellowish this particular bacteria Serratia marcescens at room temperature often has this reddish pink color to it if you grow it at 37 degrees you do not see that color it'll just be beige but you can see on the plate the streak where we went with our loop and we have growth wherever the loop had touched and also then on the slant a lot of growth down at the bottom and then it extends up along the slant towards the top oftentimes you would look at the different characteristics nice heavy growth there notice the color one of the big things you'll want to notice is the pigmentation whenever you have a bacterial culture you're working with that can give you closest to the identification of it