Experiment 3 Procedure by Benjamin Frachem

Introduction

• The focus is on part 2 of the experiment.
• The setup is complete up to step 8.

Experimental Setup

• Equipment Used:
  • 50ml Erlenmeyer flask with stir bar.
  • Stir plate operational.
  • Sparkling air unit connected to a laptop to measure pressure and temperature.

Trial Summaries

Trial 1: Standard Trial

• Components:
  • Water: 6 ml
  • Hydrogen Peroxide: 1 ml
  • Catalase Solution: 3 ml
• Procedure:
  • Add catalase solution, insert rubber stopper to start collecting pressure data.
  • Focus on initial pressure data points to determine reaction rate.

Trial 2: Increased Substrate Concentration

• Components:
  • Water: 4 ml
  • Hydrogen Peroxide: 3 ml
  • Catalase Solution: 3 ml
• Procedure:
  • Similar procedure as Trial 1.
  • Observations showed higher pressure readings.
  • Risk of stopper popping off due to increased pressure.

Trial 3: Temperature Variation

• Components (same as Trial 1):
  • Water: 6 ml
  • Hydrogen Peroxide: 1 ml
  • Catalase Solution: 3 ml
• Conditions:
  • Flask placed in a hot bath at 40°C.
• Purpose:
  • To observe the effect of temperature on reaction rate.

Conclusion

• Three trials completed with variations in substrate concentration and temperature.
• Further trials would follow the same pattern.
• Data will be shared separately or at the end of the presentation.