Back to notes
In the FACTS process, how is the flow of cells to the detection area controlled?
Press to flip
Cells are focused and sent sequentially through a laminar flow to the detection zone.
Explain how lasers are utilized in the FACTS system.
Lasers are used to excite cells, and fluorescent signals are collected and analyzed.
How is the FACTS method used in academic research?
It's applied in fields like cell biology, immunology, and marine biology.
Describe the essential process of the pre-analysis in the FACTS method.
Cells are placed in a homogenized suspension and filtered through a 40μm sieve mesh.
What unique application of FACTS technology is found in the brewing industry?
Selection of different yeast strains that influence beer flavor.
How does the FACTS system contribute to environmental monitoring?
It is used for tasks such as water quality assessment.
What are some of the devices used to collect sorted cells in the FACTS method?
Centrifuge tubes, multi-well plates, and customized devices.
What is one significant benefit of using the FACTS technology in cancer research?
It allows for the extraction, in vitro culture, and re-infusion of immune globulin cells from cancer patients.
What range of particle sizes can the FACTS instrument detect?
The instrument can detect particles ranging from 0.5μm to 50μm.
What are the components of the instrumentation in a FACTS system?
Includes laminar flow system, laser system, dichroic filters, photomultiplier tubes, and fiber optics.
What advantage does FACTS have in experiments requiring cell purification?
It offers a high-throughput detection option and targeted cell sorting for purification.
What is the main function of a fluorescence activated cell sorting (FACTS) machine?
To perform cell sorting based on fluorescence signal analysis.
What role does the FACTS instrument play in the pharmaceutical industry?
Aids in small molecule drug development, observational research, and efficacy verification.
How does the FACTS system perform high-throughput detection and analysis?
By simultaneously detecting up to nine fluorescence signals and analyzing thousands of cells per second.
In what clinical applications is the FACTS method particularly beneficial?
For tasks such as autologous immune cell therapy, cancer gene sequencing, and blood disease diagnostics.
Previous
Next