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Aspirin Overview and Chemistry

Sep 6, 2025

Overview

This lecture focuses on aspirin’s chemical structure, unique mechanism among NSAIDs, clinical uses, and important precautions regarding its side effects.

History and Chemistry of Aspirin

  • Aspirin belongs to the salicylic acid class of NSAIDs.
  • Ancient remedies used willow and meadowsweet for fever, both containing salicin, a precursor to salicylic acid.
  • Felix Hoffman synthesized aspirin (acetylsalicylic acid) in 1899 by adding an acetyl group to salicylic acid.
  • The acetyl group distinguishes aspirin, enabling its unique effects compared to other NSAIDs.

Mechanism of Action

  • Aspirin irreversibly inhibits cyclooxygenase (COX) enzymes by acetylating a serine residue in the active site.
  • Acetylation permanently inactivates COX, requiring new enzyme synthesis for function to return.
  • Other NSAIDs only reversibly inhibit COX by competing for the active site.

Clinical Effects and Platelet Function

  • Low-dose aspirin prevents blood clotting by inhibiting pro-clotting prostaglandin TxA2 in platelets.
  • Platelets cannot replace inactivated COX because they lack nuclei, so the effect lasts for the platelet’s lifespan (8-9 days).
  • Vessel-lining cells can regenerate COX, maintaining production of anti-clotting prostaglandin PGI2.
  • The shift in pro- and anti-clotting factors results in aspirin's blood-thinning effect.

Aspirin-Triggered Lipoxins and Anti-inflammatory Actions

  • Aspirin-acetylated COX-2 can generate aspirin-triggered lipoxins (ATLs) like 15-epi-lipoxin-A4, enhancing anti-inflammatory effects.
  • ATLs are epimers of natural lipoxins, differing only in one stereocenter.

Clinical Uses and Precautions

  • Aspirin treats pain, fever, inflammation, and is used in low doses for heart disease and stroke prevention.
  • Some patients, especially 10-25% of asthmatics, have hypersensitivity to aspirin.
  • Aspirin is linked to Reye’s syndrome in children and young adults with viral illness, causing liver and brain swelling.
  • Aspirin is avoided in children and young adults with viral fever; acetaminophen is preferred because it lacks this risk.

Key Terms & Definitions

  • NSAID — Nonsteroidal anti-inflammatory drug, used to reduce pain, fever, and inflammation.
  • Aspirin (acetylsalicylic acid) — An NSAID that irreversibly inhibits COX.
  • COX (cyclooxygenase) — Enzyme making prostaglandins, central to inflammation and clotting.
  • TxA2 (thromboxane A2) — Prostaglandin promoting blood clotting.
  • PGI2 (prostacyclin) — Prostaglandin preventing blood clotting.
  • Aspirin-triggered lipoxins (ATLs) — Anti-inflammatory molecules produced by aspirin-modified COX-2.
  • Reye’s syndrome — Rare disorder causing swelling in the liver and brain, associated with aspirin use in children.

Action Items / Next Steps

  • Review the mechanism of action for acetaminophen (Tylenol) in the next lecture.