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Overview of Surface Plasmon Resonance Applications
Nov 19, 2024
Lecture Notes: Surface Plasmon Resonance (SPR) and Applications
Introduction to SPR
Total Internal Reflection
: Occurs when light angles increase beyond a critical point.
Metal Film Introduction
: Causes electron oscillation due to electromagnetic waves from light.
Resonance and SPR Angle
:
Matching of oscillation wavelengths with surface magnet wavelengths leads to resonance.
At specific angles, reflected light is absorbed, creating a sharp peak called SPR angle.
Key Concepts
:
Evanescent Wave
: Energy wave trapped at the metal surface.
Surface Plasmon
: Activated electrons on the surface.
Importance and Applications of SPR
Sensitivity
: Sensitive to boundary conditions and refractive index changes.
Label-Free Detection
: Allows real-time monitoring without the need for labels.
Applications in Drug Screening
:
Monitors drug binding speed and duration.
Combined with other methods for comprehensive analysis.
Research Directions
Label-Free Techniques
: Development of optical instrumentation to address clinical issues.
Precision Drug Screening
: Targeting cell surface receptors and addressing antimicrobial resistance.
Technological Improvements
Optical Imaging Enhancements
:
Replacing photodetectors with system cameras.
High throughput and spatial resolution improvements.
Single Cell Analysis
:
Resolves single-cell molecular interactions and 3D structures.
Applications in Cancer Research
Receptor Targeting
: Focus on EGFR receptors, which are cancer biomarkers.
Binding Kinetics
:
Measures drug-receptor interactions.
Provides insights into drug efficacy and resistance mechanisms.
Tumor Progression Studies
:
Quantifies cell movement and attachment properties.
Correlates tethering properties with cancer stages.
Wearable Devices and Monitoring
Continuous Monitoring
: Development of wearable devices for real-time health tracking.
Power Solutions
:
NFC and self-powered systems using body heat.
Electrical sensors for continuous glucose and ion monitoring.
Summary
High sensitivity and versatility of SPR allow detailed cellular and molecular interaction studies.
Applications span from drug screening to cancer diagnostics and real-time health monitoring.
Q&A Section
EGFR Receptor
: Epidermal Growth Factor Receptor, linked to cell growth and cancer development.
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