Structure of Transfer RNA (tRNA)

Introduction

• Topic: Transfer RNA (tRNA)
• Source: Biochemistry, First Aid, Page Number 44
• Main Function: Assists in protein synthesis, formed after translation.

Structure of Transfer RNA

• Secondary Structure:
  • Folded structure, similar to proteins
  • Composed of a sequence of 75 to 90 nucleotides
• Terminal End:
  • 3' Prime Site: Amino acid binding site
  • 5' Prime Site

Various Roles

• T Arm (T-arm):
  • Ribosome binding site
  • Acts as a protein factory
• D Arm (D-arm):
  • Unique and specific sequence
  • Recognizes specific amino acids

Process

• Under the direction of DNA:
  • DNA -> Messenger RNA -> Ribosome -> Transfer RNA
• Charging Process:
  • Enzyme: Aminoacyl Synthetase
  • Pairing of tRNA and amino acids

Important Points

• Secondary structure, number of nucleotides, 3' site, role of T arm and D arm
• Charging enzyme: Aminoacyl Synthetase

Importance of Codons

• Start Codon: AUG
• Stop Codon: UGA, UAA, UAG

Conclusion

• Understand the structure of transfer RNA on page number 44
• Importance of start and stop codons

Additional Information

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These notes will help in understanding the structure and process of transfer RNA.