Antibody Identification Example

Introduction

• Discussing antibody identification process.
• Importance of comparing reactions and notes.
• Reminder: Antibody ID isn't done without previous testing (e.g., screening positive in AHG).

Screening Cells

• Screening cells reactions are provided at the bottom.
• Often use the same manufacturer for screening and panel cells.
• Negative rows in screening cells can assist in antibody ID.

Analyzing the Panel

• Use strategies and steps taught to analyze the completed panel.
• Questions to answer:
  • Which antigens are circled? (No cross-offs across the column)
  • Example: Big C and Jka are circled.

Low Frequency Antigens

• CW and KPa are low frequency and initially not circled.
• Importance of revisiting these after completing the panel.

Cross-Offs and Unfinished Business

• Antigens with incomplete cross-offs:
  • Big K: Needs one more cross-off.
  • Lewis A: Needs another cross-off.
  • Big S: Needs further cross-offs (homozygous).
  • M: Missing homozygous cross-off.

Low Frequency Rules

• Low-frequency antigens include CWV, Jsa, KPa, Lutheran a, and Wra.
• Rule 2: If no panel/screening cells have the antigen, rule it out (e.g., Wra, V).
• Rule 1 for Lutheran a: Single heterozygous or homozygous cross-off suffices.

Applying Rule 3 for Low Frequencies

• Rule 3: If a low frequency antigen is on the same cell as a more likely antibody, it can be ruled out.
• Suspected antibodies: Big C and Jka.

Conclusion

• Currently suspicious of having antibodies to Big C and Jka.
• Additional work needed to confirm.
• Need to complete cross-offs for certain antigens.
• Apply Rule 3 to low frequency antigens.
• Extremely rare to have antibodies against low frequency antigens.

Next Steps

• Further analysis of selected cells will be presented in another video.