Transcript for:
Microscope Overview and Parts 2/2

hey everybody let's get our learn on let's talk about the parts of a microscope this is as I've written on the left-hand side here a binocular parfocal compound light microscope binocular just tells us that we have the two eyepieces traditionally microscopes only had a single one parfocal actually tells us that all of these lenses these objective lenses are connected to one another and what that means is that if you focus them properly at the lower powers like scanning power or low power they will actually already be focused or nearly focused when you get to the hydropower and the oil immersion so it makes your life much easier the way I like to look at it is every minute you spend focusing the microscope on the early objectives is time saved at the end binocular parfocal compound light microscope compound tells us that there is a multiple lens system so traditionally simple microscopes just had a single lens system maybe a round lens or a glass ball something like that but compound might like microscopes have a second lens up in the ocular so here we have our objective lenses that have already boxed in there and we also have a ocular lens as well which oftentimes is a 10x magnification but it can be 20 it can be 25 so binocular parfocal compound light microscope because of the illuminator here the light source is actually what powers this process so it is visible light that travels through your specimen and that's what allows you to see it so visible light has a pretty long wavelength which is the reason that we can't get great resolution and magnification now getting a thousand X to 2500 X is a great thing but beams of electrons for example have a much shorter wavelength so they can get a much greater resolution and much better magnification than a light microscope so binocular parfocal compound light microscope so a microscope like the name implies allows us to see things that we cannot see with the unaided eye that's what a microscope does all right let's actually look at the individual parts here so I'll just go in the numbered order so number one we do have the Ock the lens the eyepiece that's going to give us 10 20 25 X magnification number 2 they're evolving nosepiece over here on the left hand side that's what allows us to spin the objectives from usually we see a scanning power objective a low-power objective a high dry objective and the oil immersion objective number 3 is the actual objective lenses so if those are the four lenses you have then your first objective lens the scanning power will give you a magnification of 4x the second the low-power will give you a magnification of 10x B third the high dry power which is called that because the highest magnification you can get without using immersion well hence it's dry is going to give you a 40 X magnification and the oil immersion will give you a 100x magnification but you have to remember that we multiply this number by whatever is the ocular lens so traditionally microscopes are going to have a 10x objective in the ocular so the oil immersion will give you a hundred x times 10x for a total magnification of a thousand you'll see why I don't write very much so a total magnification of a thousand X all right let me get the screen erased here so number for the coarse focus knob as you can see it looks like you have one knob but it's actually two separate knobs number four is the coarse adjustment knob number five is the fine adjustment knob so they both do the same thing that when you turn the coarse knob you're going to get the stage to move up or down quite a bit and when you to turn the fine knob it's going to move just incrementally tiny tiny bits so that's the coarse versus the fine knob but both are very important the coarse knob will get you in the right ballpark and the fine knob will actually be read to your focusing number six is the stage that's we're actually going to set the space that specimen it'll it'll sit right here and be clamped in there by the lobster claws I like to call it number seven is the illuminator that's your actual light source and in this instance being visible light since the light microscope number eight is as you can see your a couple things are pointing here the condenser and the diaphragms the condenser is going to condense the light from the light source and the diaphragm is actually will allow you to open and close the aperture to determine how much light will enter the stage and the specimen and nine is the actual physical stage there that holds the slide and that's what you move up and down side to side and forward and back to position your mic respess Ihnen so that it can be used properly all right always as always I want to give thanks to the images that we use and I hope you enjoyed this you