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Guide to Materials and Methods in Research

May 27, 2025

Notes on Materials and Methods Section in Scientific Reports

Introduction

  • Importance of understanding the organization of a scientific paper.
  • Typical structure: Title, Abstract, Introduction, Materials and Methods, Results, Discussion, References.
  • Focus of this lecture: Materials and Methods section.

Purpose of Materials and Methods Section

  • Clearly describe the method used in the study.
  • Communicate how the research was conducted.
  • Necessary for replication by other researchers.

Key Components to Include

  • Equipment:
    • Describe any apparatus or machines used.
    • Importance of detail to ensure replication accuracy.
  • Participants/Subjects:
    • Shift from 'subjects' to 'participants'.
    • Describe what or who was studied.
  • Tests:
    • Detail any tests conducted (e.g., medical, educational).
    • Importance of clear test descriptions.
  • Chemicals and Supplies:
    • Specify chemicals and other materials used.
    • Include amounts, critical for results replication.
  • Procedure:
    • Describe the procedure in detail, similar to a recipe.
    • Order of operations is important if relevant.

Writing Style

  • Paragraph Style:
    • Use full paragraphs, not lists.
    • Topic sentence, supporting sentences, and conclusion.
  • Use of Visuals:
    • Photographs or diagrams can aid comprehension.

Structuring the Section

  • Single vs. Subsections:
    • Single unitary section or subsections (e.g., Participants, Materials, Procedures).
    • Consider complexity and length when deciding.
    • Consult class or journal guidelines.

Linguistic Notes

  • Tense Usage:
    • Prefer past tense to describe what was done.
    • Alternative: present/future tense for replication instructions (not recommended).
  • Avoiding "I" and "We":
    • Use passive voice to avoid first-person pronouns.
    • Examples:
      • "I heated" becomes "The mixture was heated."
      • "We measured" becomes "The temperature was measured."
    • If passive voice is awkward, use alternatives like "the present author."

Conclusion

  • Ensure clarity and replicability in Materials and Methods.
  • Future discussions will cover the Results and Discussion sections.

Key Takeaways

  • Method section should enable replication.
  • Use formal, passive voice for clarity and adherence to style.
  • Incorporate visuals where helpful, always supplementing paragraphs.