the purpose of this video is to introduce to you some of the pipettes that we're going to be using for our a septic pipe heading portion of the lab this week the first type of pipettes are going to be stored inside of a sterile container like this one this particular one has not been sterilized but I'll show you how to take the pipettes out once it has been we have a lid here and the easiest way is to take the lid and take it off and then shake the pipettes out so that they come out a little bit past the edge and we're able to grab the tip of the pipette we can put the lid back on and put the tube back and then I will show you what parts we have on this particular pipette normally if this was a sterile pipette I would not be touching anywhere near the end here but because I'm just showing you the the parts here and explaining how to do the measurements that we'll be able to handle it normally on this particular pipette you can read from the label here that it is a 10 milliliter pipette and that it is the volumes are accurately measured to 1/10 of a milliliter and then we will notice something on here near the top of the the pipette there is an etched band sometimes these can be colored bands as well and there could be more than one of them the etched band here is an indicator that this particular pipette is what is called a serological pipet or otherwise known as a blow out pipette because in order to get the accurate volume we're blowing out all of the liquid all the way out of the pipette you also notice right here at the top there is a piece of cotton that is used to protect the pipetting device whichever one we're using to prevent any liquid or bacteria from going up into the pipetting device itself so this again is because of the etched and we look at the markings on here we could see this is a serological or a blowout pipette and look at the increments here go from zero milliliters all the way to 10 milliliters at the very end let's compare this to a another type of pipettes that we'll take out and this pipette is also a 10 milliliter pipette and it's accurate to one tenth of a milliliter notice there's no edgings and no colored bands here at the top this one also doesn't have a cotton plug sometimes they will and sometimes they won't you'll notice here that it goes from 0 to 10 milliliters there so this is what is called a measuring or drain out pipette instead because we are getting the volume from 0 to 10 milliliters here we are not ejecting liquid all the way out past the end in order to get the desired volume so I'll show you how to practice with each of these again you can notice the increments going from 0 to 10 here let me put this back in momentarily let's go back to our blowout pipette and I'm going to show you how we are going to attach it to the pipe heading device so this right here is a example of a pipetting device and it's got the end where we put the the pipette in here this is a draining lever I usually recommend students don't use this for a couple of reasons but I'll I'll explain that more when we actually transfer some volumes here and then this is the plunger that moves up and down to be able to suck liquid in and then push liquid out back out well you're going to be doing is you're going to be shoving the end of the pipette carefully into the pipetting device and give a little twist just to make sure you've got a nice secure seal on here if you get leaking with your pipette and you do back and check your seal again when we're working with blowout pipettes in order to blow out the very end of this to get rid of the the last little bit of liquid what I recommend is we actually start with a plunger a little above the bottom here so that when we actually get to the part where we're blowing things out at the end we have a little bit of pressure that we can push to push that last drop or two out so I'll show you how to actually transfer some liquids and talk about something very important later called a meniscus in a separate video so these two things right here are micropipettes these two micropipettes are actually from different manufacturers but they have the same functional range here 100 to 1000 microliters now you may recall from our unit conversions that 1000 microliters is equivalent to one milliliter so this one right here in the window is set to 1000 microliters or one milliliter this one here is set to 575 microliters or a little bit more than half of a milliliter the way that you adjust the values here in the window is to twist the plunger so we can twist the plunger to decrease the volume we can twist the plunger to increase the volume one of the things you want to be really careful with though is to not decrease the volume below the stated minimum or above the stated maximum because you will damage the pipette so just be very careful to make sure you are not trying to set the pipette outside its minimum or maximum range so let's set this one back to 575 and in order to suck up liquid it is exactly 575 microliters with this pipette we actually have to use these disposable tips so you'll notice with this disposable tip box that it's been sterilized you can see that from the tape that is color changed here and we want to be really careful to keep these uncontaminated as much as possible so when we are going to get a tip the fresh tip to be able to use for transferring volumes we're going to set it where we have we're gonna hold the pipette in your dominant hand and hang it on your fingers we're in access with your thumb here and what you're going to be doing is pressing the plunger with your thumb and you've got a nice secure hold on this you'll notice on the plunger that the plunger actually has two stops so we can go down one stop and then if I press a little bit harder I can press down to a second stop and do that again if we press down to the first stop and then the second stop I'll tell you why that's important here in just a moment because we're gonna go down to the first stop to be able to withdraw our liquid and then we're going to go down to the first off in the second stop to eject our liquid so when you're getting a tip here you would lift the lid you would press the pipette down onto one of the disposable tips and put the lid right back on we do not want this lid to come off just stay off of here because you'll be exposing it to bacteria and then as long as I don't touch this tip to anything this chip will remain sterile so we can move the box out of the way let's practice here for just a second I'll show you how this works with disinfectant beaker is if I was wanting to suck up 575 microliters of liquid I would press the plunger down to the first stop I would stick it into the liquid and then release the plunger slowly and that is 575 microliters of liquid right there so let's say we're now going to transfer this liquid back to someplace else we would press down to the plunger of the first stop and then the second stop several times to get rid of the last little drop of liquid that's in there when we're all finished we're going to want to get rid of our tip and to get rid of our tip we don't grab the tip because that would be problematic but we're gonna use this ejector button right here