How to Make a Recombinant Protein Vaccine Against SARS-CoV-2

Overview

• Objective: Design experiments to generate a recombinant protein vaccine against SARS-CoV-2.
• Learning Resource: Simulation provided by LabXchange, supported by Amgen Foundation.
• Focus: Biotechnology, Genetics, Immunology, Molecular Biology, Virology.

Key Concepts

Recombinant Protein Vaccines

• Definition: Vaccines made by inserting the DNA encoding an antigen that stimulates the immune response into bacterial or mammalian cells, expressing the protein, and then using it as a vaccine.
• Target: Focus on the spike protein of SARS-CoV-2.

Experimental Design

• Steps Involved:
  1. DNA Sequencing: Identify viral proteins.
  2. Expression Vector: Clone the DNA into an expression plasmid.
  3. Protein Expression: Use bacteria or mammalian cells to express the protein.
  4. Protein Purification: Isolate and purify the protein.
  5. Immunological Testing: Test the immune response in animal models.

Techniques Used

• DNA Ligation & Transformation: Joining DNA fragments and introducing them into host cells.
• Cell Lysis & Protein Purification: Breaking open cells and isolating proteins.
• SDS-PAGE/Gel Electrophoresis: Analyzing protein purity and size.
• ELISA: Measuring immune response by detecting antibodies.

Application

• Context: Development of vaccines for COVID-19.
• Purpose: Enhance understanding of vaccine creation and immune response elicitation.
• Impact: Aid in the control and prevention of SARS-CoV-2 spread.

Additional Resources

• Vaccine Development Pathway: Understanding clinical trials and vaccine safety.
• Types of Vaccines: Overview of various vaccine types beyond SARS-CoV-2.
• Accelerating Development: Strategies to expedite vaccine production.

Licensing

• LabXchange Standard License.

Engagement

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