D2.3 Understanding Water Potential and Osmosis

Sep 24, 2024

D2.3 Water Potential and Movement of Water

Properties of Water

  • Excellent Solvent:
    • Water's polarity allows it to dissolve solutes effectively.
    • Ionic compounds:
      • Water forms shells around ions to prevent rejoining.
      • Hydrogens in water are attracted to negative ions; oxygens to positive ions.
    • Polar molecules:
      • Forms hydrogen bonds with compounds like glucose.

Osmosis

  • Definition:
    • Passive movement of water across a semipermeable membrane towards higher solute concentration.
    • Water follows higher solute concentration.
  • Types of Solutions:
    • Isotonic: Equal solute concentration as the comparative solution.
    • Hypotonic: Lower solute concentration compared to another solution.
    • Hypertonic: Higher solute concentration than the comparative solution.
  • Water Movement:
    • In hypotonic solutions, water enters the cell.
    • In hypertonic solutions, water exits the cell.
    • In isotonic solutions, no net movement of water.

Osmolarity

  • Definition: Total solute concentration in a cell.
  • Determining Osmolarity:
    • Place plant tissue in solutions of various concentrations.
    • Measure percent change in mass to find isotonic point.

Cell Membranes and Water Movement

  • Permeability:
    • Water is permeable; solutes are not.
    • Aquaporins increase water permeability.
  • Controlling Osmosis:
    • Cells adjust solute concentration and membrane permeability.

Plant Cells

  • Cell Wall:
    • Prevents excess water entry and bursting.
    • Maintains structural integrity.
  • Turgidity vs. Plasmolysis:
    • Turgid: High internal pressure, occurs in hypotonic solutions.
    • Flaccid: Occurs in isotonic solutions.
    • Plasmolysis: Cell membrane shrinks away from cell wall in hypertonic solutions.

Animal Cells

  • No Cell Wall:
    • Risk of bursting in hypotonic solutions.
    • Risk of shrinking in hypertonic solutions.
  • Isotonic Environment:
    • Animal cells prefer isotonic solutions to maintain balance.
    • Use of saline solutions for rehydration and organ preservation.

Practical Applications

  • Saline Solutions:
    • Used in IVs to rehydrate and maintain isotonic conditions for cells.
  • Organ Transplantation:
    • Organs are kept in isotonic solutions to prevent damage during transfer.