hello students today our topic of discussion is induction right the induction caja it is a process of fixing the fate of cells by interaction with the neighboring cells though is make all right get your neighboring cells and they will determine councils cafe Tioga work on city shoes you can say organs may go develop on me right or your neighboring tissues Joe induce career uncle they are known as inducers or they are also known as organizers or of inducers or organizers right and how they induce they induce by releasing some chemicals which are known as inductors so is apparently how many discuss keyattr cell differentiation mostly names may be able latakia the fate of the cells is fixed how it is fixed by differential gene expression right you have no differentiation Makia thought the differential gene expression k hey it's me some of the genes they are turned off and some genes they remain functional so different types of proteins are produced which lead to differentiation but yeha induction me Ahmedabad curry get the fate of the cells is fixed by interaction with the neighboring cells to J&K neighboring cells only that will determine their fate and those neighboring cells which induce their development is unknown as inducers or organizers how they induce them by releasing some chemicals which are known as inductors so this process of induction it was experimentally demonstrated by Speedman in 1920 in MVP ins so he observed keep the dorsal lip of glass Topo Gigio hair so wherever it is introduced it leads to the development of nervous tissue through dorsal lip of plastic or pico can he be introduced Claire Joyce a common right now must issue develop that and its associated structures right Tononi kiyow kiyow kiyow orally gastrula say he blasts over CSKA Dawson a playa or is Co insert Kia into the belly region of another gastrula a quorum Gloria Dukey guest who lost eh meta to you Joe Dawson live from the early guest role as a dorsal lip of plaster pour go inject Giardia into the belly region of another guest room right so what did the absorb he another neural plate was induced into the belly region of the host mural plate to host a Miata whisky oberek or neural plate over individual gaiety and ultimately it led to the formation of a complete am do so here you can see in this diagram like Siamese twins the two embryos they are developed and induced embryos develop over the host embryo so what does it show that the dorsal lip of plasterboard it induces the formation of the nervous system and its associated structures right and it led to the formation of a complete I'm do it means the dorsal lip of blastopore it has done we can say potential to develop into a complete a view so that is why the dorsal lip of the blastopore is also known as the organizer so he gave the term primary organizer to this dorsal lip of blastopore so now we will discuss some examples of induction so is Mayhem sub Seppala Larry and me so dermal induction right so an experiment was conducted by New Coke in 1969 on the infeed bian last early so he what they did that they took the blastomeres or the tissue from the animal pole of the blastula and cultured them separately then what did they get they got the epidermal cells or act Odom and when they cultured the cells or explant from the vegetal pole then they found though when they cultured them individually then the gotta end or down right neck in the next step they cultured these two cells together when they cultured epidermal and dermal cells together then they got another layer in between them that is the me soda so how this miss Odom is induced so a new coat suggested that this layer it is induced by the endodermal cells and normal cells these are the vegetal cells so they induce some chemicals they secrete some inducers which convert these animal tissue into the other animal cells animal pole derived cells into the miserable cells right so because when they cultured them separately Java neoneun go separately culture kiya animal explant cor vegetal explant codon Kozyrev endodermal or endodermal or epidermal cells italy but when they cultured them together double-quick at a culture chia to in quake naily up and give it as known as mistletoe right so it means that in Joe vegetal cells and they induce the animal explant cells to develop into a new layer that is miss Odom then Dale and slack in 1987 they conducted another experiment and they suggested and they proposed that cage of vegetal cells and they are further of two types that is dorsal vegetal cells and ventral vegetal cells so the dorsal vegetal cells when they come in contact with the epidermal cells then they induce them to develop into the dorsal mesoderm or jus ventral and Radames cells here they induce the epidermal cells to develop into the ventral mesoderm right tomotommy so dom say constitution's developer they just a notochord muscle say and blood cells right to the dorsal mesoderm hey it gave rise to the notochord and muscle cells while the ventral mesoderm same but the blood cells right so here you can see that the three layers are developed animal pole pika high ectodermal cells which give rise to the nervous tissue then on the vegetal pole there are the endodermal cells and Radames as the Cadwell potay elementary canal develops or respiratory urinary system it develops from the endodermis and these are the myse Adam says dosimeter damn it will give rise to no Turcotte and my cells while the ventral mesoderm it will give rise to the blood cells right so this shows the induction so induction concur I am P and Adama says they are inducing the ectodermal cells to develop into the miss Odom so now we will discuss the induction of central nervous system an experiment was conducted by hands PMON and Hildy mangled in which they discovered that the dorsal mesoderm abhayam Niki autonomy so down on adduction to Joe Dawson miss Odom which is formed from the dorsal vegetal cells so this dorsal mesoderm when it comes in contact with the ectoderm then it differentiates it into the neural tissue or the neural plate is formed from the ectoderm so if you remember Giovanni gastrulation discuss guillotine and Phoebe ins the gastrulation cassiopeia by involution so if you remember the presumptive areas the subsequently dorsal ipkiss at McArthur Northcott very cordially so Justice involution haughty or justice apricot and plate acted on key contact me at the edge Harper the Wahhabi it induces the ectoderm to differentiate into the neural plate right so in this experiment tuna sub sapele kochia they took an early gastrula so is early gastro laka a joke perspective neural ectoderm perspective near electrode amines yeah Ariana carbonara the perspective is the carbonara the neural ectoderm piranhas but it is early gastrula earliest rule as a jargon only prospective neural ectoderm tissue Co graft Kea into the epidermal electronics too many of Kavitha a key pre Cordell me sodunke Joe part ectoderm case at contact me I got that part of the ectoderm will differentiate into nervous tissue so the remaining part of the ectoderm it will form the epidermal electronics derivatives right do you have a homely cadaques experiment me they what they dare does that this prospective neural - danke graft Kia into the epidermal ectoderm region right so this prospective epidermal ectoderm tissue it has been removed and it has been grafted with this prospective neural ectoderm what did they observe that when them do develop their neural plate was developed from this region only as it has been shown in the black color through black colour say his regions a general plate beneath every bunny and this part Jo graft key Agatha near electrode Anka it did not develop into a neural plate but it became a part of this epidermal ectoderm only right but when they did the same experiment in the late gastrula stage then what did they observe the yep they creo chelate gets reliant ammonia gastrulation keyattr if you remember this is the yolk plug or you happy involution Orion or the cod sells pre Cordell plate and other areas presumptive areas they have been all involution invagination ki ho gaya so this is the latest rola so here now when they grafted this prospective neural ectoderm brain tissue to the perspective to this act epidermal ectoderm region try to uni your neural ectoderm tissues your perspective neural ectoderm tissue ESCO graphically into the epidermal ectoderm region and this is the prospective epidermal ectoderm removed right then what did observe that in the embryo when it developed and it had two neural plates right this is the host neural plate and as the kendry neural plate was formed from this grafted tissue right so what does it show it shows that the fate of this neural ectoderm it is fixed in the latest role only not in the earliest role early gastronomy under up near electrode um TT shikou AB graft cut a hole in the epidermal electrode um region then it forms a part of the epidermal electrodermal region only but latest ulama Jabbar please go graft here to see cabin acne neural plate secondary neural plate develop okay so this type of interaction of this dorsal mesoderm or pre Cordell me so Dom be both and docile me so damn cool so this type of interaction between the ectoderm and the pre Cordell miss Odom is known as primary induction it's calm Kabletown primary induction why it is known as primary induction because this event penny manager thought icky subsequently this event takes place tikki sub Savannah inner electrode um he burnt ahead by induction of Tom Creek or Delmas are done with the electrode um so that is why it has been termed as a crime reduction but now we know kids owe me so dumb conduction mo pally okay but still the same you know name it persists mythic way for rocketry okay this is the primary interaction right