today we want to talk about tomayto police's simplified we want to start with the most advanced cell type that it appears in blood that you're used to seeing and then step back step by step to the most immature form that we see in the bone marrow itself there'll be two parts part one one has to do with red blood cell production part two will have to do with neutrophil production amano points are simplified part two granular poiesis our objectives are to identify the develop most steps of granule polices and to characterize the development changes in the cytoplasm and the nucleus of these cells you receive bone marrow so we see some fat cells in through there and we see cells in development these are the granules lights right in through here as you see this is a blood in a blood vessel another set of blood blood vessel here's some but your neutrophils that have been released from the extravascular site to divide blood vessels I he receive its Road near blue another piece of bone this is bone marrow show you some blood vessels that are run through here and also we see the granulocytes of which are developing brand-new sites all through here here and here I in the bone marrow you will see peripheral blood so we see red blood cells platelets or lymphocytes but also we see neutrophils so this is matured neutrophil with a dart nucleus lobulated nucleus within a portion thereof connecting the different lobes of the nucleus in the county registry cytoplasm but if we look at grand lo poesis we can see the mature neutrophil with lobulated nucleus and attend portion and nuclear plasm in through here our pinching the nucleus off from making the lobes as we go to or more less advanced stage we can see a kind of horseshoe shape cytoplasm still looks the same kind of gray to a less mature state the metamodel site you have either oboe or kidney shaped nucleus connie intenta to cytoplasm is still mature pretty much mature already at this stage as you go to a younger cell type them the neutrophil of malecite we have over nucleus less condensed nucleus a larger cytoplasm and the cytoplasm has basically three colors it has two pink the blue and the kind of gray indicating that they have a specific as well as nonspecific granules and then the final form is a probe malecite pro malecite oval nucleus cytoplasm but just a little bit of pink in its cytoplasm if we look at a maturity to fill and a lot of other structural characteristics of white blood cells and i in electron micrograph view we can see a lymphocytes here here and here and we see neutrophil here's a neutrophil you see the two components to the lobes of the lobulated nucleus another one here you see three lobes of the lobulated nucleus all this is one yuccas but they have different lobes we have the nonspecific granules that we're seeing as well as a little dumbbell shaped granules a little dumbbell shape granules are the specific granules that you see I in the neutrophil we also have a monocyte right in through here it doesn't have the granules that you see in the neutrophils so if we look at those from the entire scheme we have the mature neutrophils the band cells that look like horseshoe shape here and these can appear in blood if you have an infection and you need a lot of neutrophils or you can't appear and these can appear on blood and then you have the neutrophilic a metal monocyte my kidney shape still Connie's mature cytoplasm varies a little bit in cytoplasm but mostly gray in mature cytoplasm then you to Philip mallow site which has the combinations of different granule specific or any or nonspecific granules and then the pro model of site which has Asia for the craniums and the blue cytoplasm to ribosome a lot of rabbit a lot of ribosomes in the cytoplasm makes it blue and also a lot of cytoplasm in these cells and so basically we're looking for the cytoplasm going from the typical grey that we're seeing we're going to pick up some blue and then ultimately have blue and pink as you have the Asia for the craniums and there with a lot of cytoplasm the nucleus we're starting with the liability a nucleus is going to be less less condensed more oval ultimately oval shape a euchromatic nucleus in the most immature form so here we see some platelets and also we see a cell that I'm not sure if it's a Asano feel or a base of feel it certainly is not a neutral field here's a neutrophil right in through there liability knew Chris very thin piece of the nuclear component there but so lobulated nucleus and kind of create a sound custom that we have the next one we see is a neutrophilic metal malecite we see there with heads if somewhat indented a nuclear profile as we see also then you need to fill up malecite which has the combination of different blue gray and in kind of pink color which has a you filling and the specific granules but otherwise a largely a oval nucleus so we came all the way through here and now we're going to look for the pro Milo site so here we have some cells that we don't know for here some lymphocyte that doesn't have much cytoplasm you see another lymphocyte here and here some nuclei we just don't know what they are but here is a neutrophilic band cell which is one that we have it seen in the previous slide the band slip there's another band cell and through here and here's a neutrophil so these give rise due to these guys as you see here is going down to a your face we have the neutrophil metal malecite like it indented with the kidneys shape as we said before and then you have the Milo site which is a combination of different colors as we mentioned and then you have a probe Milo site which has a blue cytoplasm and over nucleus blue with some pink in there as well if we want to look at that on several cells we can see the mature neutrophil a very thin portion of the chromatin lobulated nucleus gray cytoplasm neutral band cells these are the band cells a horseshoe shape as a cell with mature cytoplasm and then you get to neutrophil up mother site largely matures on cousin but it can vary a little bit but you had the kidney being a type shape nucleus and then you have early neutrophilic melot melot site as we as we see here with a combination cytoplasm indented nuclear envelope and then you have the neutrophils monocytes moreover one combination of cytoplasm different colors you get a filigree aunt granules to get ribosomes you get specific granules in through here but it can vary in in in shape of the nucleus and there's a lot of cytoplasm with a combination of different colors a late of pro malecite both of these are promyelocytes you know this is the late one and this is a typical one that you see with a euchromatic nucleus over nucleus with largely blue cytoplasm with Asia philic granules located in there as well and if we look at this game we can see the host of the different cells that are here we can see the band cells it's at the band's cell neutrophilic band cells the neutrophilic a metal myocyte neutrophilic monocytes that are located in through here we see a couple yourself fields or basophils and in the red blood cell series we can see the pro iRiver blasts we can see orthochromatic normal blasts in through here and a polychromatic erythroblast as well so of course bone marrow is a source of Greater sites and here you can see where what is actually getting out of the out of the extravascular space and is entered the blood space here's a neutrophil that we see right in here there's another one made a couple lymphocytes that we see right through there but we can see this one squeezing out of extravascular space into the vascular space that's the end of a metal police' simplified part - thank you