The Process of Transcription

Promoter and Transcription Factor

• Promoter Recognition
  • Sigma 70 factor is essential.
  • Differences in prokaryotic and eukaryotic.

Binary Complex

• Binary Complex: Relationship of RNA polymerase and DNA.
• Promoter is cleared by transcription factor H.
• Helix opening and hydrogen bond breaking.

Ternary Complex

• Initiation of RNA synthesis.
• Processes of elongation and termination.
  • Rho dependent and rho independent termination.

Prokaryotic Transcription

• Multiple Genes: Unified transcription.
• Differences in promoters: Pribnow box and Hogness box.
• Recognition of Pribnow box by sigma 70 factor.

Eukaryotic Transcription

• Promoter Structure:
  • Pribnow and Hogness boxes at -10 and -30.
• Role of TBP (TATA Binding Protein).

Transcription Factor and Their Function

• Order of transcription factors A, B, E, F, H.
• Actions of kinases: phosphorylation.

Termination Process

• Rho Protein: Breaks hydrogen bonds.
• Hairpin Loop: Causes pausing.

In Conclusion

• Diagram Reference: TBP, RNA polymerase, and other factors.
• Educating Vedmi: Preparation for CSIR 2024 exam.
  • Discount code: VED 20.

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Summary of Notes: This lecture discussed the processes of transcription, promoter recognition, and various aspects of RNA polymerase. Differences between prokaryotic and eukaryotic transcription were also explained.