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Lab Exam 2-Endochondral Bone Formation

Jun 23, 2025

Overview

This lecture examines the microscopic structure and developmental zones of endochondral bone formation, focusing on the transitions from cartilage to bone.

Histological Zones of Endochondral Bone Development

  • The uppermost section is called the zone of resting cartilage, where cartilage cells are small and inactive.
  • Below this is the zone of proliferation, where chondrocytes (cartilage cells) actively divide and begin to organize into columns.
  • Beneath the proliferative zone lies the zone of hypertrophy, where chondrocytes enlarge and the lacunae (spaces) become bigger.
  • The zone of calcification follows, marking the transition from cartilage to bone as minerals are deposited and cartilage cells die.
  • The deepest area is ossified bone, where the cartilage is completely replaced by mature bone tissue.

Key Terms & Definitions

  • Chondrocyte — a cartilage cell involved in bone development.
  • Lacuna (plural: lacunae) — small cavities in cartilage or bone that house cells.
  • Zone of Resting Cartilage — region with inactive chondrocytes at the epiphyseal plate.
  • Zone of Proliferation — area where chondrocytes divide rapidly, forming columns.
  • Zone of Hypertrophy — region where chondrocytes enlarge and spaces widen.
  • Zone of Calcification — transitional area where cartilage becomes mineralized and starts to turn into bone.
  • Ossified Bone — fully formed bone tissue, replacing the cartilage matrix.

Action Items / Next Steps

  • Review histology slides to identify and label the four zones of endochondral bone development.
  • Prepare to discuss the functional significance of each zone in class.