Understanding Respiratory Substrates and ATP

Aug 18, 2024

Lecture Notes: Respiratory Substrates

Definition of Respiratory Substrates

  • Respiratory substrates are organic molecules that can be broken down to generate ATP.
  • Common substrates include carbohydrates (e.g., glucose), lipids (e.g., fatty acids), and proteins (e.g., amino acids).

Main Focus: Efficiency of ATP Production

  • Cells can break down carbohydrates, lipids, and proteins to produce ATP.
  • The efficiency of ATP production varies between different substrates.

Proteins as Energy Sources

  • Proteins are composed of amino acids.
  • While proteins can be broken down for ATP, they are primarily needed for making antibodies, enzymes, hemoglobin, collagen, etc.
  • Cells prefer not to use amino acids as fuel.

Carbohydrates vs. Lipids

  • Examples:
    • Carbohydrate: Glucose (C₆H₁₂O₆)
    • Lipid: Fatty Acid (e.g., C₁₈H₃₄O₂)
  • ATP Yield:
    • Fatty acids have more carbon-hydrogen bonds than glucose.
    • More carbon-hydrogen bonds = more hydrogen atoms released upon breakdown.
    • Hydrogen atoms are accepted by carriers like NAD+ and FAD during respiration.
    • More hydrogen means more reduced NAD (NADH) and FAD (FADH₂), leading to higher ATP production during oxidative phosphorylation in fatty acids compared to glucose.

Why Cells Prefer Glucose

  • Despite fatty acids providing more ATP, glucose is the main energy source.
  • Aerobic vs. Anaerobic Conditions:
    • Fatty acids require oxygen for breakdown; they are only used in aerobic pathways.
    • Glucose can be broken down without oxygen through glycolysis (anaerobic pathway).
  • Glycolysis Process:
    • Glucose (6-carbon) is broken into 2 pyruvate molecules (3-carbon each) via glycolysis.
    • Pyruvate enters the mitochondrial matrix for further breakdown.
    • In absence of oxygen, glucose is converted to lactate in animals or ethanol and CO₂ in plants and yeast.
  • Ease of Breakdown:
    • Glucose (6-carbon) is easier to break down than fatty acids (e.g., 14-carbon), making it more efficient for quick energy release, especially during anaerobic conditions.

Misconceptions in Energy Use

  • During intense exercises like fast running, the body relies on glucose rather than fats due to the anaerobic demand.

Key Takeaways

  • Fatty Acids: More ATP from oxidative phosphorylation, but requires oxygen.
  • Glucose: Versatile for both aerobic and anaerobic respiration, easier to break down.
  • Understanding the differences in energy yield and conditions under which these substrates are used is crucial.