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Male Testes and Muscle Functions

Sep 2, 2025

Overview

This lab focuses on male-specific muscles associated with the testes, their anatomy, development, and function, including how they regulate temperature for optimal sperm production.

Anatomy of the Testes and Associated Structures

  • The testes are housed in the scrotum, a skin pouch located behind and below the penis.
  • Each testis is surrounded by the epididymis (C-shaped structure) and attached to the spermatic cord.
  • The spermatic cord contains arteries, veins, nerves, and the vas (ductus) deferens, which transports sperm to the prostate.
  • The vas deferens is cut during a vasectomy (male sterilization).

Embryological Development and Descent

  • Gonads develop near the kidneys and descend into the pelvis; testes move further into the scrotum in males.
  • Testes travel through the inguinal canal, dragging blood vessels, nerves, and muscle fibers along.
  • Problems with descent can lead to cryptorchidism (undescended testicle), increasing the risk of cancer, infertility, and testicular torsion.

Temperature Regulation and Muscle Function

  • Sperm production requires temperatures 2–4°C below core body temperature.
  • Testes hang outside the body in the scrotum to maintain a cooler temperature.

Cremaster Muscle

  • The cremaster muscle is derived from the internal oblique muscle (skeletal, voluntary control).
  • This muscle raises and lowers the testes in response to temperature or conscious effort.
  • Reflexive contraction typically occurs due to cold, pulling testes closer for warmth.

Dartos Muscle

  • The dartos muscle is smooth muscle within the subcutaneous layer of the scrotum (involuntary control).
  • Contraction tightens the scrotal skin, reducing heat loss in cold conditions.
  • Relaxation loosens the skin, allowing heat dissipation when warm.

Cremaster Reflex Test

  • Stroking the inner thigh triggers the cremaster muscle to contract, elevating the testis on that side.
  • Absence of this reflex can help diagnose testicular torsion in clinical settings.

Key Terms & Definitions

  • Testes/Testicles — Male reproductive organs producing sperm and testosterone.
  • Scrotum — External skin pouch housing the testes.
  • Spermatic cord — Bundle containing blood vessels, nerves, and vas deferens.
  • Vas (ductus) deferens — Tube carrying sperm from testes to prostate.
  • Cryptorchidism — Condition of undescended testicle(s).
  • Cremaster muscle — Skeletal muscle elevating and lowering the testes.
  • Dartos muscle — Smooth muscle tightening or relaxing the scrotal skin.
  • Cremaster reflex — Involuntary elevation of the testis after stroking the inner thigh.

Action Items / Next Steps

  • Review the anatomy and function of the cremaster and dartos muscles.
  • Practice identifying these structures and their roles in temperature regulation.
  • Prepare for possible lab demonstration of the cremaster reflex test.