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Understanding Prokaryotic Cells and Classification

Oct 14, 2024

BTEC Applied Science Unit 1 Biology: Prokaryotes and Prokaryotic Cells

Introduction to Cells

  • Types of Cells
    • Eukaryotic Cells: Found in plants and animals; contain nucleus and membrane-bound organelles.
    • Prokaryotic Cells: Single-celled organisms without a nucleus; primarily bacteria.

Characteristics of Prokaryotic Cells

  • Nucleoid
    • Not a true nucleus but a single circular DNA molecule.
    • Not enclosed by a membrane.
  • Ribosomes
    • Float freely in the cytoplasm.
    • Smaller (70S) than those in eukaryotic cells (80S).
  • Slime Capsule
    • Outer protective layer.
    • Helps prevent desiccation and provides defense against white blood cells.
    • Aids in adhesion to surfaces.
  • Cell Wall
    • Made of peptidoglycan (sugars and amino acids).
    • Provides structural support and shape.
  • Plasmids
    • Circular DNA structures within the cell.
    • Contain additional genes for survival, like antibiotic resistance.

Peptidoglycan and Cell Walls

  • Composition
    • Bacterial cell walls consist of long chains of sugars and amino acids.
  • Gram Test
    • Differentiates bacteria into gram-positive and gram-negative based on cell wall properties.

Gram-Positive vs. Gram-Negative Bacteria

  • Gram-Positive Bacteria
    • Thick peptidoglycan cell wall.
    • Absorb stain during gram test, turning purple.
    • Lack an outer membrane.
  • Gram-Negative Bacteria
    • Thin cell wall with an outer membrane.
    • Do not retain stain during gram test.
    • More resistant to antibiotics due to additional protective layers.

Importance of Gram Classification

  • Helps in determining the antibiotic resistance of bacteria.
  • Guides the selection of appropriate antibiotic treatments.

Key Questions and Exercises

  • What are bacterial cell walls made from? (Answer: Peptidoglycan)

  • Why are some bacteria gram-positive and others gram-negative?

  • What structural differences exist between gram-positive and gram-negative bacteria?

  • Why is it crucial for doctors to distinguish between these types?

  • Note: Refer to diagrams illustrating the differences between gram-positive and gram-negative bacteria and the flagella aiding bacterial movement.