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Understanding Photosynthesis: Light and Dark Reactions

Apr 17, 2025

Light Reaction and Dark Reaction

Overview of Photosynthesis

  • Photosynthesis is a process that converts light energy into chemical energy.
  • Occurs in chloroplasts via two phases:
    • Light Reaction: Light-dependent process.
    • Dark Reaction: Light-independent process, also known as the biosynthetic phase.

Light Reaction

  • Definition: A light-dependent process crucial for photosynthesis.
  • Key Events:
    • Light absorption by chlorophyll within thylakoids.
    • Hydrolysis of water occurs, releasing oxygen.
    • Formation of ATP and NADPH.
  • Photosystems:
    • Photosystem I (PS-I): Absorbs light at 700 nm.
    • Photosystem II (PS-II): Absorbs light at 680 nm.
  • Process:
    • Occurs in thylakoids of chloroplasts.
    • Excitation of chlorophyll-a leads to energy production (ATP & NADPH).

Dark Reaction

  • Definition: Also known as carbon-fixing reaction.
  • Characteristics:
    • Light-independent process.
    • Utilizes products (ATP, NADPH) from the light reaction.
  • Location: Occurs in the stroma of the chloroplast.
  • Calvin Cycle:
    • Captures CO2 through stomata.
    • Converts CO2 into glucose using ATP and NADPH.

Differences between Light and Dark Reactions

Light ReactionDark Reaction
Occurs only in presence of lightOccurs with or without sunlight
Photochemical phaseBiochemical phase
Occurs in grana of chloroplastOccurs in stroma of chloroplast
Produces ATP and NADPHProduces glucose
Photolysis occurs (PS-II)Photolysis does not occur

FAQs

  • Light Reaction:
    • Takes place in thylakoid membranes.
    • Light absorbed to generate NADPH and drive protons for ATP production.
  • Dark Reaction:
    • Uses CO2 with ATP and NADPH to produce glucose.
    • Takes place in chloroplast stroma.
  • End Products:
    • Light Reaction: ATP and NADPH.
    • Dark Reaction: Glucose.
  • Timing:
    • Dark reaction occurs during the day but does not require direct light.

Additional Information

  • Plant Energy without Light: ATP produced from other sources, anaerobic photosynthetic bacteria.
  • Impact of Light: Provides energy for growth through photosynthesis.