Transcript for:
Genetics Overview and History

Title: Introduction to Genetics Lecture URL Source: blob://pdf/078e0427-09fc-488d-946e-551879c70502 Markdown Content: S-BIOL3 27a Introduction to Genetics Lecture Module 1 - History, Scope, and Applications of Genetics | 1st Semester | BSY43 History, Scope, and Applications of Genetics The traits we inherit from our parents shape our features and biological identities. Each of us carries a unique set of genes responsible for our physical characteristics. traits are determined by the combination of genes (hereditary unit) we receive from our parents with some traits being dominant and others recessive . Studying genetics will help us gain insight into not only our appearance but also the broader connections we share with our family and the genetic legacy that influences who we are. Genetics coined in 1905 by William Bateson (a British geneticist). comes from the ancient Greek word ( genetikos ), which means "generative" or "related to origin". This word is then derived from (genesis ), meaning "origin". Studies how traits are passed down from one generation to the next; how genes and DNA vary and how they interact with the environment. The Hereditary Material Before the 1950s, this concept was not well understood. But it was believed to have the following features: Capable of replication Store information Variability - capable of change, undergo variations Historical Timeline of Genetics Introduction to Genetics Lecture Module 02 This timeline revisits significant people, landmark scientific discoveries, and inventions from the 19th century to the present that have contributed to the development of genetics as a science. These events have shaped our understanding of heredity, genetic variation, and the molecular mechanisms underlying these processes. 1801 Jean Baptiste Lamarck Profession: French biologist Theory: Theory of Inheritance of Acquired Characteristics (Lamarckism or Lamarckian inheritance) Key Idea: Organisms can pass on traits acquired during their lifetime to their offspring Example: Giraffe's long neck early giraffe ancestors had shorter necks and stretched to reach higher leaves. Lamarck believed this acquired trait could be passed on to offspring, resulting in longer necks in the next generation 1865 Gregor Mendel Profession: Austrian monk, biologist, and mathematician Title: "Father of Modern Genetics" Contribution: Published results of pea plant experiments Key Discoveries: S-BIOL3 27a Introduction to Genetics Lecture Module 1 - History, Scope, and Applications of Genetics | 1st Semester | BSY43 Laid the fundamental knowledge for the field of genetics Uncovered essential rules of inheritance and their mathematical foundations Influenced understanding of transmission of traits across generations 1866 Ernst Haeckel Profession: German biologist and physician Contribution: Proposed hereditary material resides in the nucleus Key Idea: The cell nucleus plays a role in inheritance (theoretical proposal) 1868 Charles Robert Darwin Profession: English naturalist Contribution: Introduced Pangenesis Theory in his book The Variation of Animals and Plants Under Domestication Key Idea: Each part of an organism releases tiny units called gemmules which travel to the reproductive organs and influence genetic traits of the next generation Impact: Pangenesis was ultimately disproved Modern genetics revealed that inheritance is determined by genes (actual hereditary units), not gemmules Darwin is best known for his widely accepted theory of natural selection , presented in his 1859 book On the Origin of Species by Means of Natural Selection, or the Preservation of Favored Races in the Struggle for Life Later discoveries in genetics, especially the role of genes and variations, have further validated the principles of evolution Here's the continued and fully detailed Historical Timeline of Genetics , preserving all original information and organizing it clearly: 1869 Friedrich Miescher Full Name: Johannes Friedrich Miescher Profession: Swiss biologist and physician Contribution: First scientist to isolate nucleic acid , which he named nuclein Context: Conducted research in Felix Hoppe-Seylers laboratory at the University of Tbingen Extracted phosphate-rich compounds from the nuclei of white blood cells In 1874, further experiments revealed that nuclein consisted of both nucleic acid and protein Impact: Mieschers discovery laid the foundation for identifying DNA as the hereditary material S-BIOL3 27a Introduction to Genetics Lecture Module 1 - History, Scope, and Applications of Genetics | 1st Semester | BSY43 18791892 Milestones in Cell Division This period marked significant progress in understanding cellular processes, especially mitosis and the structural components involved in cell division. Key Terminologies Introduced and Defined: Chromatin: Complex of DNA and proteins found in the nucleus; condenses to form chromosomes during cell division Mitosis: Process of cell division resulting in two genetically identical daughter cells Cytoplasm: Gel-like substance within the cell membrane, excluding the nucleus; contains organelles Nucleoplasm: Viscous fluid within the nucleus; contains chromatin and nucleolus Prophase: First stage of mitosis; chromatin condenses into visible chromosomes, nuclear envelope breaks down Metaphase: Stage of mitosis where chromosomes align at the cells equatorial plane before separation Key Contributors: Eduard Adolf Strasburger Polish-German botanist Studied cell division in plants Contributed to understanding of cytoplasm and nucleoplasm during cell division Walther Flemming German biologist and philanthropist Known as the Father of Cytogenetics Observed and described mitosis in detail (coined the term in 1882) Coined the term chromatin to describe the material forming chromosomes Edouard van Beneden Belgian embryologist and cytologist First to describe meiosis at the chromosomal level in 1883 Observed meiotic division in Ascaris eggs 1888 Heinrich Wilhelm Gottfried von Waldeyer-Hartz Profession: German neuroanatomist Contribution: Coined the term chromosome Definition: Chromosomes are the compact form of genetic material present in the nucleus Impact: Provided a standardized term for the physical carriers of genetic information 1893 August Weismann Profession: German evolutionary biologist Contribution: Developed the Germ Plasm Theory Key Idea: Inheritance in multicellular organisms occurs solely through germ cells (gametes such as egg and sperm) Impact: Distinguished between germ line and somatic cells , influencing modern genetics and evolutionary biology S-BIOL3 27a Introduction to Genetics Lecture Module 1 - History, Scope, and Applications of Genetics | 1st Semester | BSY43 1900 Rediscovery of Mendels Work Three scientists independently rediscovered Gregor Mendels foundational research on inheritance: Hugo de Vries Dutch botanist and geneticist Carl Erich Correns German botanist and geneticist Erich Tschermak von Seysenegg Austrian agronomist Significance: Their rediscovery validated Mendels principles of inheritance Sparked renewed interest in genetics as a scientific discipline Marked the beginning of classical genetics as a formal field of study 1902 Clarence Erwin McClung American zoologist Proposed that specific chromosomes play a role in determining the sex of animals Walter Sutton American geneticist Theodor Boveri German zoologist Developed the Chromosomal Theory of Inheritance (also known as the BoveriSutton chromosome theory or SuttonBoveri theory ) Key Concepts: Chromosomes are the carriers and transmitters of genetic material Chromosomes are linear structures that house genes at specific sites called loci William Bateson English biologist First to use the term genetics to describe the science of heredity Also credited with introducing the following terms: Homozygote: An organism with two identical alleles for a particular gene Heterozygote: An organism with two different alleles for a specific gene Epistasis: A genetic interaction where one genes expression is affected by another gene F1: The first filial generation, offspring of the parental generation (P) F2: The second filial generation, produced by crossing individuals from the F1 generation Allelomorph (or allele): Different forms of a gene that can exist at a specific locus on a chromosome 1905 William Bateson Continued contributions to terminology and genetic theory Reginald Punnett S-BIOL3 27a Introduction to Genetics Lecture Module 1 - History, Scope, and Applications of Genetics | 1st Semester | BSY43 British geneticist Discovered genetic linkage through experiments with sweet peas Defined genetic linkage as the tendency of DNA sequences close together on a chromosome to be inherited together Also noted that multiple genes can influence the function of other genes Lucien Claude Cunot French geneticist Described the lethal gene at the agouti locus (responsible for coat color) in mice Identified the concept of multiple alleles Nettie Marie Stevens American geneticist Edmund Wilson American zoologist and geneticist Discovered the XY sex-determination system in insects Later, in the 1920s, Theophilus Painter confirmed that the XY system also applies to humans and other mammals 1908 Godfrey Harold Hardy British mathematician Wilhelm Weinberg German obstetrician-gynecologist Together, Hardy and Weinberg demonstrated the Hardy-Weinberg principle (or Hardy-Weinberg theorem ) Principle states that allele and genotype frequencies in a population remain constant over generations if no evolutionary forces are acting on it 1909 Wilhelm Johannsen Danish botanist and geneticist Introduced the terms gene , phenotype , and genotype in his book Elemente der exakten Erblichkeitslehre ("Elements of the Exact Theory of Heredity") Definitions: Gene: The fundamental unit of heredity responsible for transmitting traits from parents to offspring Phenotype: Observable physical and physiological traits of an organism resulting from interaction between genotype and environment Genotype: The genetic constitution of an organism, specifically the alleles it possesses for a particular gene 1910 Thomas Hunt Morgan Profession: American evolutionary biologist and geneticist Contributions: Discovered sex linkage in Drosophila (fruit flies), showing that certain traits are associated with sex chromosomes Along with Calvin Bridges , discovered chromosomal nondisjunction in Drosophila, S-BIOL3 27a Introduction to Genetics Lecture Module 1 - History, Scope, and Applications of Genetics | 1st Semester | BSY43 a phenomenon where chromosomes fail to separate properly during cell division 1913 Alfred Henry Sturtevant Profession: American geneticist Contribution: Mapped the genes of Drosophila within their chromosomes Created the first genetic map of the fruit fly, establishing the concept of gene order and linkage on chromosomes 1914 Calvin Blackman Bridges Profession: American geneticist Contributions: Collaborated with Thomas Hunt Morgan to observe nondisjunction in Drosophila Made key observations in Drosophila genetics: Chromosomal deficiencies (1917): segments of chromosomes missing Duplications (1919): segments of chromosomes repeated Translocations (1923): rearrangement of chromosome segments between non-homologous chromosomes 1928 Frederick Griffith Profession: British bacteriologist Contribution: Conducted the Griffith experiment (also known as the Transformation experiment ) Demonstrated bacterial transformation , showing that bacteria can transfer heritable genetic information Key Findings: Heat-killed III-S (smooth strain) mixed with live II-R (rough strain) caused death in mice Live III-S and II-R strains were recovered from the mice Concluded that a "transforming principle" from dead III-S bacteria converted II-R into lethal III-S Later research identified this transforming principle as DNA 1931 Harriet Creighton and Barbara McClintock S-BIOL3 27a Introduction to Genetics Lecture Module 1 - History, Scope, and Applications of Genetics | 1st Semester | BSY43 Professions: Harriet Creighton: American botanist and geneticist Barbara McClintock: American cytogeneticist Contribution: Published a groundbreaking paper describing chromosomal crossover Demonstrated that linked genes undergo physical exchange of chromosome segments Confirmed that chromosomes carry genetic information , and genes are located on these structures 1941 George Beadle and Edward Tatum Professions: American geneticists Contribution: Proposed the one geneone enzyme hypothesis Hypothesis states that each gene produces a specific enzyme, which influences a single step in a metabolic pathway This concept linked genes directly to biochemical functions 1944 Oswald Avery, Maclyn McCarty, and Colin MacLeod Professions: Oswald Avery: Canadian-American molecular biologist Maclyn McCarty: American geneticist Colin MacLeod: Canadian-American geneticist Contribution: Conducted the AveryMacLeodMcCarty experiment Aimed to purify and characterize the "transforming principle" from Griffiths 1928 experiment Demonstrated that DNA , not protein, is responsible for bacterial transformation Challenged the prevailing belief that proteins were the carriers of genetic information 1948 Barbara McClintock Profession: American cytogeneticist Contribution: Discovered transposable genes , also known as "jumping genes" Observed that certain DNA segments can move from one location to another within the genome This discovery revealed a dynamic aspect of the genome and gene regulation 1950 Erwin Chargaff Profession: Austro-Hungarian-born American biochemist Contribution: Formulated Chargaffs rules Key Principles: S-BIOL3 27a Introduction to Genetics Lecture Module 1 - History, Scope, and Applications of Genetics | 1st Semester | BSY43 In DNA, the amount of guanine (G) equals cytosine (C) The amount of adenine (A) equals thymine (T) There is a 1:1 stoichiometric ratio of purine (A + G) to pyrimidine (T + C) bases These findings were critical to understanding DNA structure 1951 Rosalind Franklin British chemist Maurice Wilkins New Zealand-born British biophysicist Raymond Gosling British physicist Conducted X-ray diffraction studies revealing the helical structure of DNA Franklin produced high-quality images, notably Photo 51 (taken by Gosling), indicating DNAs helical shape and dimensions 1953 James Watson American molecular biologist Francis Crick English biophysicist and molecular biologist Elucidated the chemical structure of DNA using Chargaffs data and Franklins X-ray images Along with Wilkins, Watson and Crick were awarded the 1962 Nobel Prize for Physiology or Medicine 1957 Francis Crick Proposed the Central Dogma of Molecular Biology Described in his 1970 Nature paper: 1958 Matthew Meselson and Franklin Stahl American geneticists and molecular biologists Conducted the MeselsonStahl experiment Provided strong evidence for the semiconservative model of DNA replication proposed by Watson and Crick 1961 Jacques Monod French biochemist Franois Jacob French molecular biologist Published Genetic Regulatory Mechanism Described the lac operon in E. coli , explaining gene regulation in prokaryotes 1966 Marshall Nirenberg , Har Khorana , Severo Ochoa , Robert Holley Deciphered the genetic code Revealed how nucleic acids determine the sequence of amino acids in proteins 1960s Werner Arber , Daniel Nathans , Hamilton Othanel Smith S-BIOL3 27a Introduction to Genetics Lecture Module 1 - History, Scope, and Applications of Genetics | 1st Semester | BSY43 Discovered restriction enzymes , capable of cleaving DNA into specific fragments Awarded the 1978 Nobel Prize for Physiology or Medicine 1970s Allan M. Maxam , Walter Gilbert , Frederick Sanger Developed early methods of DNA sequencing Gilbert and Sanger received the 1980 Nobel Prize for Chemistry 1972 Paul Berg American biochemist Created the first recombinant DNA molecule by splicing bacterial and viral DNA Established a general method for combining distinct DNA molecules 1982 Establishment of GenBank DNA sequence database founded by Walter Goad and Los Alamos National Laboratory Became part of the National Center for Biotechnology Information (NCBI) in 1988 1983 Kary Mullis American biochemist Invented the polymerase chain reaction (PCR) Technique amplifies specific DNA sequences from small samples Awarded the 1993 Nobel Prize for Chemistry 1990 Human Genome Project (HGP) International effort to map and understand the complete human genome Objectives: Identify all genes on each chromosome Elucidate their biochemical characteristics Spanned 15 years 2001 Publication of the Human Genome Sequence Approx. 2,900 Mbp in length Initial gene count estimate: 35,00040,000 Revised in 2002 to ~30,000 genes 2002 International HapMap Project Mapped genetic variations linked to human diseases Cataloged ~3.1 million variants by Phase II (2007) Advanced understanding of disease genetics and personalized medicine 2008 1000 Genomes Project (1KGP) Ran from 2008 to 2015 S-BIOL3 27a Introduction to Genetics Lecture Module 1 - History, Scope, and Applications of Genetics | 1st Semester | BSY43 Sequenced genomes of over 2,500 individuals from diverse ethnic backgrounds Created the most complete catalog of human genetic variation at the time 2011 DNA Sequencing of Various Organisms Organism Completed Ongoing Viruses 2,688 0 Microbes 1,710 6,085 Fungi 208 205 Animals 182 256 Plants (+algae) 47 107 2020 to Present Recent Developments Emmanuelle Charpentier French microbiologist and geneticist Jennifer A. Doudna American biochemist Awarded the 2020 Nobel Prize in Chemistry Recognized for developing a method for genome editing (CRISPR-Cas9) Svante Pbo Swedish geneticist Awarded the 2022 Nobel Prize in Physiology & Medicine Honored for discoveries concerning the genomes of extinct hominins and human evolution Katalin Karik Hungarian-American biochemist Drew Weissman American immunologist Awarded the 2023 Nobel Prize in Physiology & Medicine Recognized for discoveries in nucleoside base modifications enabling mRNA vaccine development against COVID-19 # Branches of Genetics Genetics is a broad scientific field that investigates heredity, variation, and molecular mechanisms. Each branch focuses on specific aspects of genetic function and inheritance, with applications across biology, medicine, and biotechnology. Classical Genetics Focuses on how genetic traits are inherited through reproduction Based on patterns observed before molecular techniques were developed Key areas: Mendelian Genetics Genetic Linkage Genetic Mapping Cytogenetics S-BIOL3 27a Introduction to Genetics Lecture Module 1 - History, Scope, and Applications of Genetics | 1st Semester | BSY43 Studies the relationship between chromosomes and cell division processes Includes analysis of mitosis and meiosis Investigates chromosomal abnormalities Molecular Genetics Examines how changes in DNA structure or gene expression lead to variation among organisms Topics include: Gene editing Genetic engineering Microbial Genetics Investigates inheritance and genetic function in microorganisms Covers: Conjugation Bacterial transformation Horizontal gene transfer Incorporates molecular techniques such as genetic engineering and gene editing Human Genetics Focuses on inheritance and genetic factors specific to humans Encompasses a wide range of applications studied within the human context Medical Genetics Concentrates on the genetic basis of diseases, disorders, and conditions Explores implications for patient care and treatment Population Genetics Studies genetic variation within and between populations Central to evolutionary biology Examines processes such as: Adaptation Speciation Determination of allelic and other genetic characteristics of populations # Scope and Applications # of Genetics Genetics has broad applications across multiple fields, from agriculture and medicine to law and biotechnology. These applications demonstrate how genetic principles are used to solve real-world problems and improve quality of life. Plant and Animal Improvement Selective breeding is used to choose parents with favorable traits Offspring are more likely to inherit and express those traits Widely applied in agriculture and livestock production to enhance yield, resistance, and quality Medicine Involves: Studying inheritance patterns Mapping disease-related genes Diagnosing and treating genetic disorders S-BIOL3 27a Introduction to Genetics Lecture Module 1 - History, Scope, and Applications of Genetics | 1st Semester | BSY43 Providing genetic counseling to patients and families Applications include personalized medicine and gene therapy Legal Applications Genetics is used in: Criminal investigations (e.g., DNA fingerprinting) Paternity disputes and family law cases DNA evidence helps establish identity and biological relationships Genetic Engineering Enables mass production of medical products through recombinant DNA technology Examples include: Insulin Human growth hormone Follistim (infertility treatment) Human albumin Monoclonal antibodies Antihemophilic factors Vaccines Other therapeutic drugs # Practice: Applying # Genetics in Forensics Scenario #1 Murder Investigation Five DNA samples collected: One from the victim One unknown sample Three from suspects Task: Compare the banding patterns in the gel electrophoresis image Goal: Identify which suspects DNA matches the unknown sample by aligning the bands (Note: Image analysis required to determine match. If you upload the gel image, I can help interpret it.) Scenario #2 Paternity Test Individuals tested: Jane (Mother) Joe (F1) Thomas (F2) Child Task: Compare DNA bands of the child with those of Jane, Joe, and Thomas Goal: Determine which man shares the most matching bands with the child, excluding maternal bands Conclusion: The biological father will share all remaining bands with the child that do not come from the mother (Again, image analysis is needed. If you provide the gel image, I can help identify the father based on band patterns.) # Lesson Summary Introduction to Genetics Lecture Module 02 Historical Timeline of Genetics Genetics began in the mid-1800s with Gregor Mendels pea plant experiments, establishing the basic principles of inheritance. S-BIOL3 27a Introduction to Genetics Lecture Module 1 - History, Scope, and Applications of Genetics | 1st Semester | BSY43 In the early 20th century, discoveries about chromosomes and DNA expanded this foundation. A major breakthrough occurred in the 1950s when James Watson and Francis Crick identified the double helix structure of DNA , using data from Rosalind Franklin , Maurice Wilkins , and Erwin Chargaff . This discovery revolutionized our understanding of heredity and propelled genetic research forward in the decades that followed. Branches of Genetics Genetics is divided into multiple specialized branches, each focusing on different aspects of heredity and variation: Classical Genetics : Studies inheritance through reproduction; includes Mendelian genetics, genetic linkage, and mapping. Cytogenetics : Examines chromosomes and cell division (mitosis and meiosis), including chromosomal abnormalities. Molecular Genetics : Investigates how changes in DNA structure or expression lead to variation; includes gene editing and genetic engineering. Microbial Genetics : Focuses on genetic mechanisms in microorganisms, including conjugation, transformation, and horizontal gene transfer. Human Genetics : Explores genetic traits and inheritance patterns specific to humans. Medical Genetics : Studies genetic causes of diseases and disorders, with implications for diagnosis, treatment, and patient care. Population Genetics : Analyzes genetic variation within and between populations, contributing to evolutionary biology. Scope and Applications of Genetics Genetics has wide-ranging applications across science, medicine, and society: Plant and Animal Improvement : Selective breeding is used to produce offspring with desirable traits. Medicine : Involves studying inheritance patterns, mapping disease-related genes, diagnosing genetic disorders, and providing genetic counseling. Legal Applications : Used in criminal investigations and paternity cases through DNA analysis. Genetic Engineering : Enables mass production of medical products such as insulin , vaccines , human growth hormone , monoclonal antibodies , and other essential drugs. Addresses healthcare demands and medication shortages globally.