Understanding Photosynthesis Process and Mechanisms

Oct 16, 2024

Photosynthesis Lecture Notes

Introduction to Photosynthesis

  • Photosynthesis Definition: Process using light energy to build carbohydrates.
  • Equation: 6H2O + 6CO2 + light β†’ C6H12O6 + O2
    • Reactants: Water (H2O) and Carbon Dioxide (CO2)
    • Products: Glucose (C6H12O6) and Oxygen (O2)
  • Process Summary:
    • Water is absorbed via roots.
    • Carbon dioxide enters through stomata.
    • Oxygen is released through stomata.

Key Organelles

  • Chloroplast: Site of photosynthesis
    • Contains chlorophyll (pigment that absorbs blue and red light)
    • Reflects green light (why plants are green)
    • Structure: Contains thylakoids (site of light-dependent reactions)
  • Mitochondria: Responsible for cellular respiration (opposite of photosynthesis)
    • Converts glucose and oxygen back to CO2 and water

Photosynthesis Stages

1. Light-Dependent Reactions

  • Occur in the thylakoid membranes
  • Convert light energy into chemical energy (ATP and NADPH)
  • Key Reactions:
    • Water is oxidized to oxygen gas
    • NADP+ is reduced to NADPH
    • ATP is generated from ADP and phosphate via ATP synthase
  • Photosystems:
    • Photosystem II (P680): Absorbs light at 680 nm, oxidizes water
    • Photosystem I (P700): Absorbs light at 700 nm, re-energizes electrons

2. Light-Independent Reactions (Calvin Cycle)

  • Occur in the stroma
  • Use ATP and NADPH from light-dependent reactions to reduce CO2 into G3P (glyceraldehyde-3-phosphate)
  • Steps:
    • Carbon Fixation: CO2 is fixed by reacting with RuBP (ribulose biphosphate) catalyzed by rubisco, forming 3-phosphoglycerate (PGA)
    • Reduction Phase: 3-phosphoglycerate is phosphorylated by ATP and reduced by NADPH to form G3P
    • Regeneration of RuBP: G3P is used to regenerate RuBP, continuing the cycle
  • Outputs: For every 3 CO2, one G3P is produced

Summary

  • Overall Photosynthesis Process: Involves both light-dependent and light-independent reactions
    • Light-dependent reactions produce ATP and NADPH
    • Calvin cycle utilizes these to fix carbon into glucose
  • Energy Requirement: Requires 18 ATP and 12 NADPH to produce one glucose molecule

Important Points

  • Chlorophyll absorbs specific wavelengths (blue and red) and reflects green
  • Photosystem II is crucial for oxidizing water to oxygen
  • Calvin Cycle is central to carbon fixation and sugar production in plants

Conclusion: Photosynthesis is a complex but essential process converting light energy into chemical energy, forming the basis of the food chain.