DNA Extraction Experiment Lecture

Presenter

• Dr. Jeffrey Tro
  • Senior Vice President of Operations at Mir Genetic Labs

Overview

• Simple kitchen-based experiment to extract your own DNA.
• DNA is the code of life and codes for everything you are.
• The process involves five simple steps using common household items.

Materials Needed

• Table Salt (Sodium Chloride): Required for creating the salt solution.
• Water: Use a clear glass or beaker.
• Measuring Tools:
  • 1/4 cup and 1/2 cup measurements.
  • Graduated cylinder for precise measurement.
• Containers:
  • Clear glass for saltwater and gargled solution.
  • Disposable cup for gargling.
• Dishwashing Soap: Two drops needed.
• Rubbing Alcohol (70% Isopropanol): For the final step.

Steps to Extract DNA

1. Salt Solution Preparation
   • Combine 3 teaspoons or 1 tablespoon of table salt with 500 ml (approx. 2 cups) of water.
   • Stir until the salt is completely dissolved.
2. Gargling Step
   • Measure 50 ml of the salt solution using a graduated cylinder.
   • Use a disposable cup for gargling.
   • Swirl and gargle the solution in the mouth for 1 full minute.
   • Spit the gargled solution into a clear glass.
3. Add Dish Soap
   • Add two drops of dishwashing soap to the gargled solution.
   • Stir gently to avoid creating bubbles.
4. Add Rubbing Alcohol
   • Pour 100 ml or about half a cup of rubbing alcohol into the solution.
5. Observation
   • Wait for 3 to 4 minutes to see the DNA precipitate.

Experiment Execution

• Volunteer: Michelle is the guinea pig for this demonstration.
• Outcome: In about 3–4 minutes, you can observe the extracted DNA, described as the "code of life."

Conclusion

• This experiment is a fun and educational way to visualize your own DNA using simple household materials.
• Encouragement to try at home.