Transcript for:
Introduction to Biology Concepts

Just a note on all narrated lectures you might be viewing throughout the semester. Most of these lectures have been abbreviated when we are going through the narration and that's just so you're not spending a lot of time watching the video on the computer. With that in mind, always make sure that you go back and read through the powerpoint presentations to catch up on some more of the fine details that might be skimmed over or may not be elaborated on during this narrative lecture presentation. Now in this first chapter, as we begin this view into biology, we are going to look at some of the themes that this textbook in particular uses to kind of guide you down the path of understanding what biology is and how we can explore biology in different frameworks. So, first we want to emphasize the fact that with biology, we are looking at an underlying principle or theme of evolution and evolution itself we're going to see later on has a different definition than we're giving you today. We're going to start just by saying evolution is this process of change and essentially one of the things as biologists that we look for in the world around us is how have life forms changed, how have plants, animals, fungi produce help any one of those things change in terms of our ability to live on this planet now later on as we get back into evolution in this chapter and then later on in the semester uh you will see that we give evolution a more defined definition and we will say that beyond the process of change it's also considered this descent with modification essentially the same idea still we're looking at the fact that as we do go on to reproduce generation by generation we are hoping that our offspring are changed in some way or modified in some way to make sure that they are adapted to their environments and capable of surviving in those current conditions so as we look at other things throughout the semester evolution is one of the ideas that will always be kind of in the back of our understanding and we will try to almost always connect your quality chapter link back to that core idea because it's our basic basis of understanding for a lot of biology in terms of why things work the way they do or how things are going to function in that living world so as we begin this journey with biology though we want to first kind of start out with very basic ideas and the first thing is is what is biology what are we looking at if we are a biologist and the most basic definition for biology is simply studying life and with that study of life we also have to think about what is something that's alive and we could say why the most basic form that it's doing something you think about a rock versus say a plant a plant can undergo photosynthesis a plant can take up water a plant can respond to the environment in terms of maybe adjusting leaf position for the sunlight it can respond to varying conditions with nutrients or predation a lot of things that it can do the rock in the other hand though really doesn't do much it might absorb energy but it's not going to process it it might have some change over time through weathering but it's not really doing that process so we said that life itself in general is recognized by doing something and we'll come back and emphasize kind of why that doing something is critical especially in the case of things maybe like viruses which may not fit our complete definition of what life is but we can still see that they do something in terms of this simplicity so in terms of things that can be done by life forms you can see these several properties or some of the things that every life form on this planet has to undergo and if we think back to evolution as the underlying principle underlying theme we can always start here with reproduction the fact is that every organism out there is kind of ingrained in their genetics that they want to reproduce they want to carry on their genetic line the next generation and that's the one thing that will allow evolution to continue is the fact that we are making more individuals every single generation uh to take our place or to carry on that line of genetics for that species so everything else beyond the reproduction kind of will follow suit and the fact that if we reproduce we've got to have growth and development if we're trying to grow and develop features you've got to build a process energy to make sure that's going to the right areas to grow thicker skin or to grow larger body size you also have to have the process of regulation and that does tie into our energy processing but also regulation in terms of things like temperature regulation of water supply regulation of even food supply you know at food supply will go along with energy processing as well but regulation basically means that we're going to maintain a set of conditions oftentimes we could say as a homeostasis where you're maintaining the conditions that allows the organism to survive in those conditions the other thing we can look at is the fact of ordering this is actually one idea or one property that we will come back and revisit as we move into our energy chapter and talk about the rules of thermodynamics and one of the things we will point out is that with thermodynamics or the flow of energy within a system you will see that energy itself tends to want to go to a more disorganized or disordered state but as a living system we have the capability of again processing energy in such a way that we can utilize that to actually go against that disorder or the process of trying to make more unorganized forms and we instead will go through and make order which is what's going to allow us to develop in the process of our reproduction the process the growth the process even of regulation the last few properties here will tie back into that coordinate evolution and that's the fact that as we respond to our environment we also have this ability to adapt as well so this venus fly trap responding to the environment uh where it needs a greater input of nutrients uh than what the actual soil can provide it's adapted to have a type of trap mechanism to gather the insects which means it's going to have a way to lure them in a way to trigger the trap and a way to digest them so that in itself is an evolutionary adaptation it's an adaptation that is based on a response to the environment where it needs to be able to go through and maintain to build and survive those conditions again the one core thing out of all these life-giving properties does come back to this factor reproduction in fact we think about these later on we're going to see that when it comes to defining what a life form is made of in this case called the cell we're going to see one of those key properties is the fact that a cell has to be able to go through this process of reproducing or this life form of reproducing to make more of those cells now all of this understanding of biology is actually going to focus along what we call the biological hierarchy essentially with the hierarchy we're going to look at in a framework where we can move from the most basic structures so looking at some very small atomic forms or molecular structures even to our cell configuration and from there we can move all the way up into understandings of the entire planet now throughout our course we're going to focus primarily in this bottom section of the biological hierarchy in fact we'll spend the next couple of chapters probably chapters two through five uh looking at the structure of the atom and the molecules that we can form from there and then as we're moving to chapter five look at the macromolecules we'll start to hit off and go into some of the organelles which we'll see later on in chapter six and some of those features that we're gonna form into our cell configurations now as we do branch off into other forms like reproduction or looking at evolution as a whole we do momentarily take a glimpse into some of this population organism structure with a hierarchy but most of the introductory form for biology is looking at these core principles because if you want to understand what happens at a community level you've got to have the basic understanding of what's happening down here in this molecular and this cellular form now one thing that we do hope to see and it'll be quite evident as we go into our chemistry is the fact that as we move from level to level as we go from one smaller piece to a larger piece we will see what are called emergent properties and essentially emergent properties are taking what is already there and allowing it to arrange in such a way to produce some new functionality so we have the example here with a bicycle and all the parts if we give the instructions and we assemble all those parts correctly you get a functioning bicycle to emerge but if you happen to misconnect a piece or you leave a piece out or you rearrange a different way you may not get a functioning bicycle but you might have something else maybe a pulley system maybe a unicycle instead of a bicycle but you're going to find that as you rearrange these smaller bits and pieces it does provide a capability of having some new functionality as you move up into larger and larger forms now another thing we're going to look at again this is going back to the idea of that ordering within our structure in the thermodynamics is the fact that for a life form to survive and to reproduce or to grow and develop it's got to have a way of taking energy in and transforming it or transferring it from form to form now typically when we look at most of our living systems think back to that hierarchy there we talked about the planet or you can see a little level down below maybe the community or ecosystem we would say that most energy will flow into our ecosystem as a source of sunlight in this case sunlight is a form what we call kinetic energy it's motion as that energy makes its way through things like the plants out of the consumer or sorry as a producer and then into maybe a consumer like an animal it's ultimately going to leave that ecosystem this case leaving our planet per se as a form of heat heat is also a form though of kinetic energy now in amongst all those conversions you will find that it's not kinetic energy all the way we do go to other forms like potential energy this is based sometimes on the chemical structure of an item or even the location of some of these forms so structure and location would be our potential energy motion we're going to see as kinetic energy now we'll come back and revisit this idea in more detail as we get into our metabolism and looking at how energy itself can be processed through an idea like respiration or a process through a form like photosynthesis and we're going to see how each one of those will use the energy as a way of transforming or transferring it forward to form within our system so to give you an idea of kind of how we approach biology especially in terms of things like energy or even going back to kind of biological hierarchy we have a very basic diagram here of the cycling of elements or chemicals in our system as well as the flow of energy in our environment and you can see that ultimately here the energy comes in as this kinetic energy or the light energy and it's going to leave as this heat which is also kinetic energy in those processes of transfer or transformation though in between you do have some potential energy in many cases be in the chemical structure or the chemical energy but any motion an organism does so a caterpillar climate of the plant that's also a conversion back into kinetic energy based on the motion that's being produced now where the light energy itself is useful especially to our producers this heat later on we're going to see is not as useful in fact this is one of the items that we will go back in to show that in terms of thermodynamics or this flow of energy that is actually one of things maintaining the order that we want to see within our ecosystems now the other thing about we talked with say those molecules as the lower level of the biological hierarchy is the fact that if you want to examine a population of organisms or a community or even just say a tissue structure one of the critical pieces is understanding where these chemicals come from and where they're going to end up so in this case for a plant that's growing and utilizing that solar energy it's bringing up the chemicals from the soil in terms of the nutrients so maybe nitrogen or phosphorus or even the water as part of a chemical being brought in but those materials will be returned either through the dropping of a leaf the dying of the plant or even the excrement of a caterpillar that's fed on that leaf tissue gets returned back down to our soil and things like our microbes which are decomposers will return it back to a more basic form so going from more complex structure down to more simple one and part of this process for that chemical cycle now another thing we'll get into which is apparent right off the bat in chemistry is the fact that as we look at the organization of this biological hierarchy the structure of an item just in general is highly correlated to its functionality so we just saw a plant gathering sunlight there in our last diagram if that blade of that leaf is very flat or so the leaf surface is very flat that's going to maximize how much light can be converted through our photosynthesis uh the process by capturing it with a chloroplast if we look at the bird wing at the bottom we can see the bird wing itself is a really light bone but it's also very rigid and it's what's going to allow that bird to achieve flight due to a lighter structure but also rigidity to withstand those forces it's also the same thing we're going to see our chemistry if we're looking at a structure of a carbohydrate or a protein or even a lipid that structure is going to correlate to its functionality whether it can dissolve easy with water or not dissolve with water whether it can store high amounts of energy or low amounts of energy these are one of the things especially as you move through our chemistry that we're going to look at in greater detail to show how the structure itself if it is changed is going to potentially alter the functionality of that form so going back to our first idea with biology in terms of understanding that it's the process of describing what living things essentially can do if we want to give a better definition beyond just doing something to what a life form is we're going to see that every life form out there has two basic kind of things it has to do the first one we saw was reproduction and that's what's going to fall into most of our organisms the second part though is the fact that all life on our planet is typically defined by having a cell configuration now before we go further in looking at that cell we do want to take one kind of side step here and think about a virus we're going to see later on a couple of our little examples probably in other chapters that a virus itself does not have the same features that a cell does and so we talk about later on here that a cell has a membrane you're not going to see this same kind of membrane-bound structure in a virus now a virus will have genetic material either dna or rna but the last thing that this virus is not going to have is the capability of cell free production it does reproduce but it reproduces by using another organism as a way to direct that reproduction so think about viruses again as a general idea we could say that viruses do something they're able to make more of themselves by hijacking another cell but it's not kind of the true level of life we normally consider where we have a cell configuration and they can reproduce on their own so every bacteria every produce every plant every animal every fungus they're all going to have a cell configuration that can reproduce now when it comes to describing that cell for these life forms we can go very basic so we have a membrane that's going to enclose it keep it all nice and safe and we have the genetic material typically going to be in the form of what is called dna now we can add on two more to this idea of what builds a cell and essentially the reason why we want to add on two more features here is the fact that with just a membrane in dna that cell doesn't have much functionality so when we add in the last two one of which is what's called the cytosol or the cytoplasm you're going to find that that now gives us ability to support the inner structure of that cell we could also add in the final feature called the ribosome and the ribosome is a structure within the cell that can convert or translate the dna which is our genetic information into a functional product typically in the form we call a protein now again having these extra pieces of the cytosol or also the cytoplasm and having the ribosome this will make it so that that cell is now not just a structure that can store material but now it's a structure that can store information and can convert the information into doing something as a functional product so membrane and dna basic start parts of our cell but adding in the cytoplasm or the cytosol and the ribosome that makes it complete to do all those different properties so reproduction the growth the development the response that's what's going to make that a truly functioning system in terms of self-configuration now we'll see later on as we come back to the cell in chapter six that there are two main categories of cells that consist of a life on our planet we have the much larger and sometimes considered more complex eukaryotic cell so this is our large one we see on the diagram here and we have the much smaller a lot of times we call simpler prokaryotic cell which we see up here kind of representation of a bacteria now before we go into these in great details in the next chapter chapter six we will see that in this basic understanding for chapter one we want to see the difference between these two as the fact that a eukaryotic cell has this nucleus this is the place where that cell is going to store the dna in the prokaryotic cell there is no nucleus you're simply going to have the dna in what is called the nucleoid region essentially means there's no membrane surrounding that dna inside that cell nothing to protect or nothing to separate in the eukaryote though you've got the membrane which acts as a barrier to separate this fluid in the cytoplasm here and the rest of that cell content now looking at these two cells you will go back and see kind of the basic functionality of what a cell was both have a membrane so membrane on this one here membrane over here both have the dna both have the cytoplasm the lighter brown and what's not labeled though on here is the factor ribosomes and you can probably consider here all these tiny tiny little specks all throughout that lighter tan with cytoplasm those essentially are the ribosomes that help to carry out this process with our transformation of the dna inside that cell into a functional product like a protein and they're over here in the bacterial cell as well so looking at eukaryotes and prokaryotes in the big takeaway between these two essentially is the eukaryote has a nucleus it's this membrane enclosed structure called the organelle and the prokaryotic cell lacks the nucleus and it lacks any kind of membrane balanced structure in fact one way to remember this is the fact that part of the name here the karyote corresponds to a actual nucleus so the eu in the eukaryote corresponds to true so true nucleus here for eukaryote where in the prokaryotic or the prokaryote cell we have pro being before so before nucleus so no nucleus in these small bacterial type of cells versus habit of nucleus and things like our eukaryotes what you consider things like the animals the plants the fungi and the protists so because that dna is universal among all of our cell types out there you will find that dna itself is this one piece that again going back to kind of evolution is part of our process of modifying life on the planet and part of the process of ensuring that life can adapt and survive if conditions change now as we talk about dna in the upcoming chapters especially going back to our cell configuration you're going to see several different ways of visualizing this information dna itself is the most basic form it's going to consist of a double helix in terms of the structure if we look at a sub part of that dna we can look at what are called the genes and the gene essentially is a small little set of instructions within that dna that says this is what you can carry out so eye color hair color skin color production of an enzyme to carry out digestion of a sugar these are all sets of information that are carried within that dna molecule as a unique actual set of instructions called the gene now if we condense down that dna into a much more consistent packaging we can go from dna to what is called the chromatin to alternate what is called the chromosome and the chromosome was one way that we can actually visualize uh this information in terms of seeing where it is in that cell environment this idea we'll come back to as we get into cell division later on where we will show how dna itself can go through a transformation into the chromatin and then into the chromosome but you're not changing any information you're basically just repackaging so this little double helix over here we're seeing this essentially is your dna molecule you've got these two strands and those two strands have this core consider what's our structural backbone of sugar and phosphate and coming off of that structural form we have these individual letters the a the c the t and the g and essentially these individual we call nitrogenous bases make up the main part of how nucleotides which are the subunit of the dna are going to store information so when your body says that you've got this gene that's going to make an enzyme one way or somebody else has it make it another way or you've got blue eyes and somebody else has green eyes it all comes back to the sequencing here in these letters and it could be a simple change that instead of being a c t it's a gt causing that change in that sequence so these four letters that we see here the a the g the c and t this is the information that stores everything within the dna based on just four letters now of course there's a whole process in trying to convert those four letters into functional products like proteins and that's something we'll explore later on what is called a gene expression towards the anabor our semester now one side stuff we're going to do here just for a moment as we think about the idea of genes and regulating processes is the fact that in our system if we think about the idea of the regulation as one of those properties the dna is regulating what our our cells are doing and it's kind of controlling them based on what instructions are being read or not read and as part of that process uh we will see that a lot of the sequences of the dna that we carry out in terms of convert to functional material is used to make what are called enzymes and enzymes really are kind of the workhorse within our system if we want to convert a product that we consumed so a large mass of carbohydrates into an output of smaller sugar molecules or even some like atp which is the fuel for our cells we're going to use these typical we call metabolic pathways we're converting material from one form to the next one and the way that we can convert from say material a to b and b to c and c to d is using different enzymes now in these little metabolic pathways there are two ways of regulating whether we make more or less of that final product and the majority of our system relies on what is called a negative feedback regulation and essentially here as you build up this end product the product itself is going to come back in and hinder or block or stop this initial enzyme will be able to convert the first set of material into the second set and essentially what this does is it makes the body use up whatever remaining end product it has before more of it is made so you're going to slow down the process in terms of that production now in the positive feedback pathway it's the reversal and there aren't quite as many of these in our system in terms of regulating a metabolic pathway but for this one now the end product itself is going to speed the process up and it's going to make more and more of the end product allow that to accumulate now we think about this as a general idea we'll come back again focus this more in our metabolism chapter the first one makes more sense for negative feedback and part of our body is that we don't want to waste energy we don't want to waste material if we're not going to use it so negative feedback allows us to maintain that regulation we can maintain that homeostasis of our body to make sure things are processing correctly but in some scenarios say the production of milk to feed a baby or the process of trying to clot a wound we want to have that positive feedback we want to make more and more of that product because it's needed at that point in time there through that process again this kind of idea of feedback regulation kind of an odd time we bring it up in our chapters but it's one of the things that we think back to the idea of what dna is and what the genes are this actual regulation itself does come back into play with the enzymes because the enzymes here what's going to cause these pathways to be able to convert material material and produce these end products so going back to our evolution idea because this is one of the core themes that we will see come up in pretty much every chapter in the very beginning we said that evolution is this process of change that's modifying life on our planet we can also say that evolution itself is defined as a descent with modification and so essentially you're going to see that every time you reproduce as an organism you're hoping to modify your offspring what we call descendants and to show that those modifications are going to make them better suited to their surroundings now you as an organism right now you are a modified descendant of a common ancestor so if we look back to say one small little bit on the evolutionary tree of human race you're going to find that as a human a homo sapien you are not the only only homogeneous has ever existed uh you're going to find that there have been multiple other homo genuses that have existed in terms of evolution but we're the one that has made it thus far in terms of a descendant from a common ancestor you could also find that as descendants we are linked back along the primate line to a common ancestor that also branched off to produce the modern-day chimpanzees modern-day gorillas modern-day even orangutans if you want to go that far but those are all based on the fact that evolution is describing how the modern form has been modified from those previous generations so with evolution what we're trying to show is that first of all there's a great unity among all life and going back to that dna you're going to see that because dna is every one of the living forms out there whether it's bacteria whether it's plant whether it's animal whether it's fungi that's a unifying feature but you're also going to find because that dna can change simply in what letter is there for the a's the g's the c's and t's that also leads to the great diversity and the fact that you can have organisms surviving uh in the soil organisms surviving in water organisms surviving in complete darkness order and surviving and all kinds of habitats that is all based again on the dna that unifies them but also creates this diversity among organisms now one of the things we'll see later in evolution is that as an idea there needs to be a process behind it that is driving this descent modification or deriving this change and that process is what is called natural selection natural selection is an idea or this principle that basically demonstrates the fact that an organism is selected for or resulted against by the environment essentially what the natural selection idea is trying to show is that as an organism you are suited to your environment in order to allow you to survive you have this adaptation so you can see here that the beetles in our bottom picture initially have a whole range of colors you've got light ones you've got dark ones you've got some in between now this essentially shows the population starts out or at this point in time has a variety of traits now one principle of natural selection that has to always be there is that those traits that we're seeing have to be able to be inherited so if they'll pass on to the genetics now when you're bringing a selective pressure like a predator in this case the bird this is now showing that the environment the predator's case is selecting for the lighter beetles and as you go by generation by generation those lighter beetles are being removed allowing the darker beetles to proliferate and they're reproducing more and more each generation as the darker beetles the only ones primarily reproducing you're going to see a shift in the population having a greater dark beetle consistency due to the pressure in this case of that predator eating these prey items so natural selection as a process here you see that the environment is selecting in this case for a certain condition but you will notice that even though it's looking for the darker color it's not a hundred percent change every single beetle is the exact same color there's still variation and that's because the environment itself is always going to be changing and as it changes it's got to have variation there within that population in order for it to be able to go with you and select the lighter one or the darker one or the larger or the smaller the more spot of the left spot or whatever that variant might be there has to be variation there in order for natural selection to go through and select were to favor one trait over the other now we'll come back to these core principles with evolution natural selection again towards the end of our semester and we'll see that a man named charles darwin is really kind of the founding father with these principles laying out the framework to how all this works and kind of drives our process of understanding as to what biology is and how the world around us is going to work based on evolution through natural selection now one thing we can look at here just as a general idea is kind of the diversity of life so we said unity is that dna and we said diversity comes from how that dna is going to transform the different parts of the organism now this idea of diversity brings us into what's considered taxonomy and taxonomy essentially is the branch of biology or the sectional biology that looks at classifying these species into their groups based on similarities now with taxonomy uh you're going to find that this is probably the only time all semester that we'll focus in this great detail on it we'll probably come back and look at a little more as we examine some of the kingdoms of our eukaryotes but for the most part taxonomy is reserved in terms of the actual understanding and the actual use of this would show and diversity more in a bio2 course where it looks at the different arrangements with the microbes with the plants the fungi and the animals but with taxonomy one thing that you're going to focus on is the fact that as you look at a more all-encompassing part of the taxonomic group in this case you're the domain the domain is a much greater inclusion of the organisms so in this first one here with the domain eukaryote you have pretty much every single eukaryote on the planet you've got the produce you've got plants you've got the fungi you have all the different animals so quite a diversity amongst other organisms as you keep narrowing down your focus though essentially what's the goal of our taxonomy to the species you start to eliminate different features so going from the main eukaryote to the kingdom animalia you're moving the plants the fungi and the produce they're changing what some of the characteristics are or from the kingdom animalia to the phylum chordata or down to the class mammalia or all the way down to our species versus americanus that we're defining based on a key set of principles that shows that it's different from the polar bear that grizzly bear but they still share this commonality now this is also one way we think back to evolution to show how we have again a diversity we've got that distinct species versus unity so all among this domain eukarya they're unified by the future of having in this case a nucleus or the kingdom animalia all unified by the feature that they're going to have to go out there and find their food in some way or another now in our taxonomy we do want to emphasize the fact that there are three main domains in terms of classifying the life of the planet the first two are both prokaryotes so these are two organisms that are going to not have a nucleus they've got dna but there is no membrane-bound organelle or no membrane enclosed structure to store the dna and these are also very small or microscopic organisms now on the hand side here the orange this is our domain bacteria on the right hand side here we have the domain archaea and essentially these two organisms if we looked at the scale of the magnification here you're probably seeing in the range of maybe about at least maybe 2500 times magnified for the bacteria maybe 5 000 times magnified in the case the archaea but these are two distinct domains of life on our planet and even though they look very similar in terms of how small they are the fact that they lack a nucleus they're quite different in terms of the diversity and one of the biggest differences is the fact that the archaea tend to be more of extreme individuals so leaving in very hot environments or maybe a very acidic environment or maybe a very chemical rich environment where the bacteria sometimes live in those conditions but tend to be generalists that live in a wide variety of conditions in fact probably right now in your body your bacterial cells will outnumber your animal cells and that's just because the number of bacteria around us are there in such high quantities now one thing about bacteria is that they tend to usually get the bad rat in terms of bacteria not good for us and that's because there are some bacteria out there that do cause problems but you as an organism along with all of our ancestors and evolution would not be here today if it wasn't for the bacteria because bacteria essentially are going to make us individuals who we are today and allow us to survive based on how we interact with our bacteria or how the bacteria can transform materials in the way that we use them within our system now beyond those first two domains of the bacteria in the archaea which are prokaryotes we go into the main eukarya and these are all organisms that are going to have a nucleus so that's the unifying feature as you start to branch out from that domain eukaryote though in the kingdoms we get into the plants the fungi and the animals now these first three divisions of domain eukarya into these three kingdoms are based on how food is obtained so plants are going to go through and make their own food by photosynthesis fungi are going to absorb their food basically move it right across their cell membrane and animals have to go out there and ingest their food so i have some kind of mouth-like structure or some kind of hole to bring that food in from the environment now the last little kingdom here part of our demandia carrier is the protist and you'll notice that we don't say the protos as being classified by one particular way of finding food or another and that's because within this protist kingdom you're going to find some that are more similar to animals and the way they ingest their food some are more similar to the fungi in terms of absorbing their food and some more similar to the plants which i'm going to go photosynthesis but you will find that in the produce kingdom here that these are organisms that do not quite fit with the other kingdoms and now if at one point in time they were part of the different plant fungi and animal kingdoms but based on the current taxonomy we had them separated as their own group and a lot of times they tend to be very small microscopic organisms but there are some as a multi-cell structure like say our algae particularly in the kelp along the pacific coast of north america they are ones that even though not plants they look like plants maybe they are actually multi-cell protists so they're quite large in size but they're part of that protestant all right now the last little bit of this chapter is based on more of the discovery approach uh and so kind of looking at how do we examine the world around us and what kind of methodology would we use to show how things are going to function so because biology is part of this much larger grouping of kind of understanding basically part of a science you will find that any one of the parts of science so looking at chemistry looking at geology looking at biology is basically this search to know more such as science itself means to know so as part of a biologist's goal you're going to find that we're always in the search of understanding trying to figure out why things are happening the way they are so part of this idea of inquiry is forming some kind of process to go through and give us a better methodology to go through the process of trying to figure out what in fact is happening in the world around us so one of the first steps in scientific method is making observations this is one of the things that even if you're saying you're not a biologist you actually do on a regular basis and essentially as biologists our goal in observations is describing what is happening around us with the natural structures so looking at the plants looking at the fungi looking at the animals looking at even more detail the insects and essentially trying to understand or trying to describe what they're doing so let's say you're a farmer and you go out to your field and notice that your field is wilting well let's make an observation you're now going to go out about the process of trying to remedy that why that crop is wilty or let's say you're sitting at home listen to this lecture your dog is next to you and your dog starts to go through and throw up a whole bunch of grass who knows you're gonna describe that process to happen right next to you there and never try to figure out maybe why your dog is storing up or maybe why your dog is peeing on the floor who knows but said you're trying to describe this natural phenomena so if you're a chemist you're trying to describe why the atoms maybe come together a certain way physicists trying to describe why certain forms of energy transfer one way versus another essentially those observations you're making are the very first step in understanding what is happening around you now as you go about making observations the second idea you have to think about is how are you going to support the observation how are you going to go through and classify what is happening based on trying to give support later on and that comes to data essentially data is a way for us to go through and make a record of what the observation was so take an idea of you know the crops that are wilty you might go through and make a description you might describe the color of the leaf uh you might describe the conditions of the environment how hot how cold how dry how wet whatever it is you might describe that as a scenario but you also might go through and do what is called quantitative data and make a recorded measurement so beyond describing that it's hot or cold you might take an actual measure and say that it's you know 27 degrees celsius or maybe it was 110 degrees celsius who knows but you're going to make a recorded measure which then you can go through and use as an analyzing technique trying to show why things have or have not changed based on the observation now this approach of collecting data is also one thing you'll do through your lab exercises throughout this course and you will find that in most of the lab exercises we rely on quantitative data it's one of the easier ones that we can do and it's one that we can put into a nice little table or a nice little graph to show the results so if you record the measurement of a change in temperature or a change in weight or you go through and look at the difference in heights of individuals these are all quantitative data measurements but you will find in some experiments that having qualitative data is just as important so instead of actually measuring how many molecules are made maybe we do a description i'm going to say that this is a lighter color or darker color and that color then corresponds to the relative number of the molecules that were were not present in that experiment was being done now beyond the whole idea of making observations and deciding on what kind of data might help you support that we now move into what is called inductive reasoning and inductor reading essentially is a conclusion that you will come up with based off what you have been exposed to in the past and so for everyone this idea of inductive reasoning is a little bit different you might find that as an individual if you are older in your years or if you're you know not as experienced and you're younger you have different ways of viewing the world around you but essentially inductive reasoning allows you to draw a conclusion about the observation you're seeing without having to do any kind of experimentation so for example if we look to the east every morning this inductor of reasoning within us will tell us that the sun should be rising in the easterly direction because we can see it every day if something looks to the west at nighttime more expect the sun to be setting at a certain time of day there it's repeat observation that we are seeing that says okay oh yeah we know we're looking at the sun in the morning it should be easterly direction we see it at night it's a westerly direction we could also take it in this different framework if you've got a child and let's see that child in the kitchen and you've got a hot burner on the stove the likelihood that child will touch that burner versus say you as an adult touching that burner is probably much greater because that child has not experienced that much life around it in that case if the child does touch the hot burner they're going to form some inductive reasonings that says well that stove could be hot sometimes and that means the next time they're around the stove they might have a better chance of making observations maybe not going near it maybe staying away from it maybe even putting a handle because if it's warm before they actually go and touch something and that's basically what we do as every individual even if we're not biologists we go about our life forming these inductive reasonings that we can draw upon to help us figure out why things are happening the way that they are so from the observations from the data and inductive reasonings we now go into forming a hypothesis an essential hypothesis is an explanation to a question asking about the observation around us we can best say that this is a tentative answer so essentially a hypothesis is just a blanket statement so let's say your dog is throwing up next to you your hypothesis might be well my dog ate some bad food or if your plants are wilting my plants have not gotten enough water these are very simple explanations as to why something is happening but with a hypothesis though you want to be able to go out and test it so you wouldn't say necessarily that your dog is puking or throwing up because there are ghosts inside of it making it throw up or that your crops are wilted because the ghosts have gone through and there's a spiritual awareness in your in your plants causing them to be upset and they're wilty we can measure water we can measure intake of food for the dog we can do all those things but we can't go off and examine that kind of spiritual or that ghostly presence so hypothesis done by this scientific method has to be one that we can go out and test we can do observations or experimentation on those ideas hypothesis itself essentially your answer to what you're observing so to give a really quick example in this scenario here we've got an observation that a flashlight is not working now even though we are looking at this based on biology on a regular basis your mind is carrying out this kind of scientific investigation every time something around you is not working your brain goes into this automatic mode which essentially is make an observation that is a problem leading you right into the the idea of asking a question why this is happening and then hopefully down the path of trying to find an answer so in this case with the observation the flashlight not working you might automatically say well i dropped it in the water so maybe that's why it's not working or i haven't used it in two years maybe the batteries are dead or my dog chewed on it and there's you know saliva different often maybe that's why it's not working so you're going to go through this idea of making up these statements or these ideas as to why you're seeing this observation right away now in the true scientific kind of mind frame though we have to provide all these explanations i have to do one at a time in terms of testing to figure out which one is causing the problem which one will give us an answer as we're seeing for the observation so in the idea of the flashlight here if our first explanation is that the batteries are dead we can make a prediction if you take the batteries out put new ones in just fix the problem that means that we can test the stent take all batteries off putting batteries in if we come out to our conclusion and it doesn't work you're going to see that this test in this case is going to falsify the hypothesis we could also reword that we could say the test failed to support the hypothesis so once that first hypothesis doesn't pan out we can go with second idea maybe the bulb is burnt out so we say okay the explanation in this case the bulb was burnt out that's our our statement prediction replace the bulbs to fix it so take the old bulb up a new bulb back in that's our testing here and it works so in this case we could say that the test does not falsify hypothesis or that our data we collected the observation that we have like at the end of the flashlight is supporting this hypothesis for this point in time you will notice however though that none of those hypotheses showed the word proof and that's because especially in biology proof is a very strong word proof for biologists means that it's going to be the same thing every single time based on that data collection so if we took the batteries out put them back in it didn't work and we said that this test proves our hypothesis wrong as biologists in our mind we say well that means never mean the batteries if we said the bulb proves a hypothesis to be correct we might say that every time the ball is gonna be the problem but it's not and that's because when you're looking at this kind of idea of the flashlight here even though it's not a living system for us you're gonna find there are many variables many other things that could have happened to lead us to this change in the flashlight working so maybe for the batteries maybe you put them the wrong way maybe with the bulb the actual flashlight wasn't screwed all the way down tightly there are many things that could have caused this to work beyond the battery beyond the bulb that we didn't see or account for in that scenario well that's the same thing for most science we come out do a day experimentation we collect the data we say yeah okay well this supports it this doesn't support it but it's not a concrete set of evidence that will be there every single time now of course if we do the experiments over and over and over again we will start to go to understand that based on our observations we now have what we call deductive reasoning and deductive reasoning basically establishes that if we have some known premises so we have these known ideas that are widely supported by lots and lots of data that we can combine these premises to make a new prediction so it's kind of like going through and using what we already know and saying okay well if these two are established what happens when you combine them does it hold true does it come out to show that it does work so inductive was based on what you'd already learned deductive now is saying okay we know this what can we learn now from those new premises so in the example we can see here that premise one is that organisms are made of cells so we'd have a checklist that say what an organism is okay oh cells are on there for premise two we would say humans are organisms again we have a checklist that describes what maybe the human is or what the organism is that will allow us to support that understanding your new reasoning then is a fact that well humans then should be composed of cells that's our prediction so you can go out there and test it go out to take a skin sample take a cheek sample take a blood cell look at that and say yes we have composition of cells now if we modify this idea just a little bit if we said that organisms are made of cells keep that one the same and we said aliens are organisms we could then say that aliens are composed of cells so based on that prediction if we found some alien life form and it comes out to show that there really aren't any cells there this means that one of our two premises has to be changed or modified and that's the whole part about biology in terms of being a science it's always got the possibility to change we go off and look for evidence we try to support it and it works for a long time but if something new shows up which means we have to go back and change the understanding change our thought process as to why that may have happened again with your hypothesis it's got to be testable it also means that we can't use supernatural or religious explanations because it's outside the bounds of why we're explaining why things are happening all right last little part of this chapter then is give a nice little example about how this might apply to a real world scenario and for this one the scientific investigation is using the idea of mimicry uh in organisms in this case mimicry in a snake population and the general idea behind this experiment is that there are poisonous snake species out there and the poisonous or say venomous ones tend to be very brightly colored to warn the predators that they need to stay away there are also mimics though which have the same coloration but don't carry that venom so our scientist henry bates here had a hypothesis that the mimicry itself evolved in that harmless or non-venomous species as an adaptation so a modification to reduce that chance or in this case to increase their chance of surviving in those habitats so this hypothesis was tested with two snake species we have the dangerous one the venomous eastern coral snake and we have the non-venomous of the scarlet king stick now both these snakes have a very similar colorations they're going to be red black and yellow but the pattern is slightly different now the other thing for most memory to actually work is that these species have to be in overlapping ranges so for this one we're looking at north and south carolina and you're going to find there are areas where the non-venomous king snake overlaps with that venomous coral snake and there are areas where the king snake is all by itself and there's no overlapping range with that venomous one so based on that hypothesis bates comes out with the prediction that the inheritance of that avoidance pattern for that coral snake coloration should cause then those kink snakes be attacked less frequently if in fact the present where those coral snakes were present there as well so looking at our little area of study we've got north and south carolina you can see the range here of the non-venomous king snake is much greater than the range here of that coral snake now the coral snake we're going to see is again pretty similar coloration we've got the black yellow red the king snake though has it as a yellow red black now as a child i was taught kind of the phrase that red on yellow deadly fellow or some might say kill a fellow and i said red on black was friendly jack now looking at the coloration itself here if you take the standpoint of a predator let's say a bird of prey or a larger animal you're probably not going to sit there and examine the exact pattern if you have inherited this annoying evolution's pattern and you see this bright coloration or just a pattern in general you might say well no i'm going to avoid that i'm not going to go near that structure if you don't have that known avoidance though you might see this bright coloration and say well gee there's lack of camouflage i've got free dinner right in front of me because it's standing out against that background now as part of the experimentation then you're going to find that we take that prediction and we design the setup and so with this hypothesis we're going to have two different groupings we have the control group which is one we're going to use as a baseline to see how many attacks there are on this kind of configuration with this clay and the actual shape and we have the experimental group which is actually going to resemble in this case the banding pattern of that king stick now in terms of the setup we're going to put same numbers in each one of these species out there at multiple field sites and we're going to put them in areas where there's overlapping range of those snakes and areas where the king snake is not overlapping with that coral snake now as part of the setup you will see that we have what are called independent variables and dependent variables the independent variable is important because this is what you decide as a researcher that you want to manipulate in this case the coloration so banded versus just brown and the location are ones that we can manipulate we could have also gone through manipulated size length shape all kinds of things but you want to keep to a very limited set of variables that you're going to change because the more variables you change the less of a chance you have a certainty of seeing why the results come back from just one of those variables now the dependent variable then is what we're going to measure so this could be again that qualitative versus the quantitative data in this case we're going to use the quantitative data which is the number of attacks on that snake and that variable we're choosing or that we're measuring and is directly linked to both the coloration and location so the snakes were put out there and after four weeks they're retrieved and they count the number of bite or claw marks on there and the data itself does support the prediction of the hypothesis and they were shown that those banded snakes had less attacks on them if they were in that same region where those venomous coral snakes were present but if those snakes that had the abandoned pattern weren't areas where the coral snakes were not present you're going to see a much greater attack rate on that manning pattern because there's no known avoidance of that coloration now this kind of experiment helped us to show that in any kind of investigation it's important that you control your experiment basically means you want to control as much as possible and only change a limited set of material minimum of of the actual variables so in this case the variable of color the variable of the location those are the only two we're going to say as contributing to why we had more or less bite marks now we could have gone and done this with just abandoned snake the problem here is that if we don't have a baseline to compare two there's no way of saying whether or not it was the banding that caused the bite marks whether it was the coloration whether it was the clay whether it was maybe even a scent coming off those snakes but with having that control group we can use that control group and say whatever happened with those bite marks is now basically eliminated and we can show that if we had more or less bite marks on that experimental one we can say that's attributing that to the change we made in that experiment now of course we can control only so much here it does not mean that we have gone through and limited any kind of variable whatsoever but you must always try to limit as many of the variables as possible so weather could have been a part of this could have had a predator population size being part of this uh you could have had even just the scent of the clay in the center of the paint that was put on the clay as a way that changes this kind of experimentation again the goal here in this kind of experiment is to show that we can collect data and potentially support our hypothesis now for this to really be a sound experiment we want to be able to go back and do this again and again so we want to go back and have the experiment run again and we want to see that these results are repeated over and over again to show that this experiment was in fact a valid one and being supported by the data that we collected now if we do this enough times and we start to develop kind of a trend with this concept eventually enough hypotheses in the same kind of general idea can lead to what is called a theory and the biggest difference between the theory and hypothesis is that the hypothesis is really narrow in terms of what it's trying to examine so in this case the snake mimicry there were those two snake species a theory though is much broader it wouldn't just be snake species it would have to be all animals or maybe all life forms in general showing that we can show data or have data that supports that again and again the idea of hypotheses though is that we can have these which lead us to new investigations which lead down to new hypotheses and essential hypotheses are what makes up most of our understanding they're kind of an educated guess based on what we collected from the data to show that this is how this is happening in terms of that scenario a theory though is one that uses widely accepted and it's one of these that has so much data behind it that nothing else is shown to negate that idea so we're going to see the evolutionary theory is really one of our biggest understandings we've got with biology later on we'll also discuss things like the cell theory uh we'll see some in terms of what's called the endosymbion theory there's other theories we will see in biology but a lot of biology still is based on hypotheses it might be supported by large amounts of data but we're still not at the point of saying that well this is the exact cause because we're are we discovering new species we're always discovering how things are happening around us and it's always a constant kind of process of trying to figure out why things are happening the way that they are so that will include our end of chapter one for us and this kind of introductory chapter just to give you a heads up as you how other chapters are kind of organized and kind of how we're going to do material throughout the semester um as we do move on though into our second chapter with chemistry we will start with a very low level there for our biological hierarchy and then start to move up further and further trying to show how things start to rearrange and produce some of those emergent properties you