today's lab is testing for macromolecules okay the four macro molecules are carbohydrates proteins lipids and nucleic acids in today's lab we will be testing for carbohydrates proteins I but not nucle gas and these who are necessary for life and they're in all living things okay and for today's lab don't forget to wear safety glasses and pull back your hair okay now on to the chest okay so one of the tests we'll be doing today is a simple sugar test and so what you'll use is benic solution and then you'll put you'll add heat to that and then it will change it in orange color and then another test will be is testing for proteins and you'll add PC solution and then it will turn purple if there's proteins present and then we'll go onto our liping test and so like the first thing you'll do is put lipid like the you'll rub the lipid on a paper on the green paper and it'll have like a translucent St and then another test we do for lipids is you'll put lipids and water like in a tube and Shake It Up and they actually won't mix but then you put lipid and ethanol in a tube and then they'll mix it and they'll make like things that look like glass be and then the last test we'll be doing today is starch test and put iodine and then the iodine will turn black because there starch pres so to start off with you will need clean test tubes and then there'll be a sugar solution you will test a gel solution you will test a vegetable oil solution you'll test a starch solution you will test and then you use bendex solution to test if there's simple sugars in it you'll use the um verret regain it and it will be purple if there's um proteins present and then ethanol you'll be ethanol in the green paper you will use part of the lipid stest and iodine you will use to test if there's starch in there and also this um this is hot water that we will use to use add the heat to the bendex solution to tell if there's simple sugars in it or not okay so I guess first get your clean test tube make sure there's nothing in it and then you'll add just a tiny like a small amount of sugar solution like not very much like that that's probably all you really need you don't really need that much okay and then to the simple sugar go add Benedicts and just add like about that equal amount into there so like that much and then do good and then you'll put it in this heat and so you'll just kind of like 30 seconds in there just Stir It a tiny bit to help it go but be careful and as you can see it's starting to change colors it's starting to go lighter cuz there are simple sugars present and pretty soon here it'll turn orange and there we go now it is orange because there's simple sugars in it okay so what you do after that is you dump out your chest tube and you clean it okay after you've cleaned out your test tube we now will test for um proteins so in the gelatin solution you just add just a tiny bit like you don't really need a whole lot like do a little more that but that much should be good and then you use the get to test it and then so just add about equal amount of that that that should be good and then you'll notice it's like purple because there's proteins in it it will turn a purple color okay now you jump out your tube again and rinse it out you probably should rinse it out okay so we're going to start off with then we're going to do lipy test and so the first one you'll do you'll get um a green paper and you'll just get some vegetable oil and you'll just rub it on there put it all over rub it on there and it you'll notice that it's translucent like you can see light through it and then if you'll notice that's different than the water one CU if we put water on it just over water is not trans translucent but then the vegetable oil is okay and so that's what you okay so now you're going to do add the vegetable oil and water in the tube so just get a little water add it in the tube and then you'll add little vegetable oil just about the same amount to it and then you like to try and mix it together and stir it as hard as you can and it won't mix so the the vegetable oil and the water won't mix but we're going to get another clean too for that but what we're but if you put the vegetable oil so now we're going to do the other test of itg which is the ve theet vegetable oil and ethanol and so just put vegetable oil now in the tube and then come over here use ethanol put that in the tube and then when you try and mix this mix it and then you'll notice like little kind of beads like glass Look beads that like resemble glass are in there and that's because they actually will mix okay okay so the last one we're going to do is your starch test and so what you do on this one is you'll put the starch solution in there okay and then after you have the starch solution you'll go over here and do the iodine just add some iodine and it will turn black because starch are present okay and then once you're done and then after this you will test these that are known like things that you know such as peanut butter milk apple juice egg whites potato corn chips bread and the and then you'll also test the unknown and Within These you're going to like test them and use all the different tests on them to figure out what macro module is the most present in them don't forget to fill out your laboratory report and fill out every single information laboratory reports you and then don't forget to clean up the supplies and put them up a [Music]