this time molten egar is used and the sample to be examined may also have been prepared by serial dilution the AAR should be kept molten at around 50° C in an incubator or water bath label the base of your empty Petra dishes with all the relevant details described previously before you start when you are ready remove the molten egar from the incubator or water bath and allow it to cool a little further you can roll the bottles in your hand to gauge the temperature it is the right temperature once it is just comfortable to hold too hot and it will kill the inoculum too cold and it will start to set in the bottle working close to the Bunton as usual an inoculum is added to the AAR using aseptic technique with this method an inoculum as great as 1 ml or 1,000 microl can be used which is 10 times that usually used for spread plating the bacteria and agar are mixed gently to avoid any bubbles which would spoil the plate once mixed the AAR is poured into a star Petra dish and allowed to set check the AAR has set before the plates are inverted and incubated as usual after incubation as per usual generally overnight the colonist will be found both on and inside the agar while this method allows for growth of anerobic as well as aerobic bacteria any obligate arrowes which end up within the agar will not grow