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Overview of Cell Biology Concepts

Apr 22, 2025

Summary of Cell Biology Lecture

Microscopy and Cell Structure

  • Cells: Fundamental units of life.
  • Light Microscope: Can view cells and nuclei but not subcellular structures.
  • Electron Microscope: Provides higher resolution and resolving power allowing detailed views of organelles.
  • Magnification Calculation: Magnification = Image Size / Object Size; hence, Actual Size = Image Size / Magnification.

Types of Cells

  • Eukaryotic Cells: Have a nucleus containing DNA (e.g., plant and animal cells).
  • Prokaryotic Cells: No nucleus, DNA found in plasmid rings.
  • Common Structures:
    • Cell Membrane: Semi-permeable, maintains cell integrity.
    • Cell Wall: Found in plant cells and bacteria, provides rigidity.
    • Cytoplasm: Site of chemical reactions.
    • Mitochondria: Site of respiration.
    • Ribosomes: Site of protein synthesis.
    • Chloroplasts (in plants): Photosynthesis occurs here.
    • Vacuole: Stores sap in plant cells.

Bacterial Growth

  • Binary Fission: Bacterial reproduction method, doubling every 10 minutes.
  • Culture Techniques: Use of aseptic techniques on agar in Petri dishes, can test antibiotics.

Genetic Material and Cell Division

  • DNA Structure: Eukaryotic cells have DNA in chromosomes, 23 pairs in humans.
  • Mitosis: Process of cell division for growth and repair, resulting in two identical cells.
    • Duplication of genetic material and organelles.
    • Division of nucleus and cellular structures.

Specialization and Stem Cells

  • Cell Specialization: Cells adapt for specific functions (nerve, muscle, etc.).
  • Stem Cells: Unspecialized cells, potential for treating diseases (e.g., diabetes, paralysis).
  • Ethical Considerations: Use in cloning and medical treatment.

Plant Cloning and Conservation

  • Use of Cloning: To prevent extinction and develop desirable crop traits.

Transport Mechanisms

  • Diffusion: Passive movement of particles from high to low concentration.
  • Osmosis: Diffusion of water across a membrane.
  • Active Transport: Energy-requiring process moving substances against a concentration gradient.

Practical Experiments

  • Osmosis Experiment: Using potato cylinders in sugar solutions to measure mass change.
  • Active Transport: Transport of minerals into root hair cells using energy.

Conclusion: The lecture covers fundamental understanding of cell biology including structures, processes, and practical applications in life sciences.


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