ELISA is a biochemical assay used in immunology to detect the presence of an antigen, antibody, or other proteins.
The acronym stands for Enzyme-linked Immunosorbent Assay.
Principle of ELISA
Involves an antigen or antibody binding to a specific counterpart attached to a solid support.
Detection is achieved using an enzyme-linked secondary antibody that binds to the primary antibody or antigen.
The enzyme's presence can be detected through various methods such as:
Spectrophotometry
Fluorimetry
Chemiluminescence
ELISA is highly sensitive and can detect minute levels of antigens or antibodies.
Applications
Measurement of Protein Concentrations: Commonly used to measure protein concentrations in body fluids like serum or urine.
Formats
Different formats of ELISA may be used depending on the specific application and detection method.
Sensitivity
Known for its sensitivity, making it suitable for detecting low concentrations of biological molecules.
Related Resources
Additional resources and related topics available on platforms like Osmosis, including topics such as amino acids, protein folding, cell cycle, DNA damage, and more.
Useful for students and professionals in fields like Medicine, Nursing, Pharmacy, and Dentistry.