Overview
This lecture explains the standardized technique for safely handling mice during laboratory procedures, focusing on proper tail picking and scruffing.
Standard Mouse Handling Technique
- Catch the mouse carefully by the tail, preferably near the base.
- For very small mice, you may grip further out along the tail.
- Place the mouse gently on a secure surface, such as your hand or a flat platform.
- Allow the mouse to grip the surface before proceeding.
Scruffing Procedure
- Gather a substantial amount of loose skin (scruff) at the back of the mouse's neck.
- Rotate the mouse and use your little finger to hold its tail securely.
- Maintain a firm but gentle grip to prevent escape and minimize stress.
- Use this secure hold for procedures like urine sampling or injections.
Completing the Process
- After the procedure, return the mouse safely to the surface or its cage.
Key Terms & Definitions
- Scruffing — Grasping the loose skin at the back of a mouse's neck to immobilize it.
- Base of the tail — The area closest to the body where the tail begins.
Action Items / Next Steps
- Practice the proper technique for tail picking and scruffing mice.
- Review safety guidelines for animal handling in your lab manual.