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ABG and Human Physiology Overview

Jun 28, 2025

Overview

This lecture concludes the course on understanding arterial blood gases (ABGs) by integrating key concepts into a unified model of human pathophysiology, with a focus on oxygen content, delivery, and ABG interpretation for clinical diagnosis.

Oxygen Content and Delivery

  • Arterial oxygen content (CaO2) equals oxygen bound to hemoglobin plus oxygen dissolved in blood.
  • CaO2 ≈ 1.34 × hemoglobin concentration × O2 saturation; dissolved oxygen is negligible.
  • Oxygen delivery (DO2) to tissues is cardiac output × CaO2.
  • Cardiac output depends on heart rate, intravascular volume, contractility, and afterload.
  • Regional DO2 = regional blood flow (Q) × CaO2.

Unified Model of Pathophysiology

  • Primary aim: human physiology maintains aerobic respiration by O2 delivery and CO2 removal.
  • Four core domains analyzable by ABG: acid-base balance (Henderson-Hasselbalch), ventilation (alveolar ventilation equation), oxygenation (alveolar gas equation, AA gradient), and O2 transport/delivery (O2 dissociation curve, CaO2, DO2).
  • Intravascular volume and cardiac function are also significant, affecting stroke volume and perfusion.

ABG Analysis Approach

  • Start with oxygenation: check AA gradient, compare to expected, check saturation gap (<5% normal).
  • Assess acid-base status: check pH, PaCO2, then compensation, anion gap, and delta ratio.
  • Develop differential diagnoses for findings, using history, labs (lactate, ketones), and imaging.
  • Synthesize findings into a final diagnosis that ties abnormalities together.

Case Summaries and Interpretation

  • Case 1: Elderly woman with fever and distress—elevated AA gradient hypoxemia, respiratory alkalosis, high anion gap metabolic acidosis; diagnosis: septic shock from pneumonia, with lactic acidosis.
  • Case 2: Unconscious middle-aged man—normal AA gradient hypoxemia, respiratory acidosis, high anion gap metabolic acidosis, metabolic alkalosis; diagnosis: drug overdose (alcohol, opiates, benzodiazepines), alcoholic ketoacidosis, lactic acidosis, dehydration.
  • Case 3: Comatose man with AIDS—normal AA gradient, high saturation gap, concurrent respiratory alkalosis and metabolic acidosis, high and normal anion gap acidosis; diagnosis: methemoglobinemia (Bactrim overdose), lactic acidosis (aspirin/methemoglobinemia), acute kidney injury, respiratory alkalosis (aspirin/methemoglobinemia).

Key Terms & Definitions

  • CaO2 — Arterial oxygen content: total O2 per 100 ml of blood.
  • DO2 — Oxygen delivery: amount of O2 delivered to tissues per minute.
  • AA Gradient — Difference between alveolar and arterial O2, indicating oxygenation efficiency.
  • Saturation Gap — Difference between pulse oximetry O2 sat and ABG-calculated sat; >5% suggests abnormal hemoglobin.
  • Delta Ratio — Value to distinguish mixed acid-base disorders in elevated anion gap metabolic acidosis.
  • Methemoglobinemia — Condition with oxidized hemoglobin that impairs O2 delivery.

Action Items / Next Steps

  • Practice ABG interpretation using structured approach outlined.
  • Review key equations: Henderson-Hasselbalch, alveolar gas equation, CaO2 formula.
  • Complete assigned readings or sample case analysis as directed by instructor.