hello everyone a very very good morning to all of you I'm a visible ml live give me a minute to confirm if I'm live if I'm visible I will start the session ahead yes is my visibility audibility everything is good give me a minute am I visible I'm not audible to you guys is someone there to confirm my visibility audibility it's good yes yes it's working thank you thank you so I hope it's working I welcome you all for today's session a very very good morning to all of you I am Dr Priyanka sachdev here and today I am here to take a very very very important topic from General microbiology that is culture media just a second so today I am going to teach you important topic from General microbiology culture media and from this topic many many mcqs come in your exam in your competitive exam and this topic person is very important for second Prof MBA students who are going to write their University exam this year right so we will be continuing with this topic let me start culture media so I will be telling you in this uh session the classification of culture media the various types of culture media and the examples of each of them and the images you know yes Ram s uh also this session is very important for fmg because you know many questions come nowadays image based questions are coming from culture based media you should be able to identify from the image which culture media is it so you have to learn three things number one the classification number two in each class the examples and of each class the image of that so if you learn that you will be able to solve all the mcqs based on this um particular topic and you will be able to write a theory also if you're a second prop student on this topic so let me start so basically what is the culture media it can be solid it can be liquid it is a medium which contain nutrients so we provide nutrients to bacteria to grow you you got a point so basically we will grow bacteria in these culture Medias they can be solid they can be liquid now the point is that why we provide nutrients to bacteria to grow why we want to grow a bacteria now just suppose this is the specimen of my patient the specimen of my patient it can be blurred it can be urine it can be CSF it can be stool it can be saliva it can be anything it is a specimen of my patient and I am suspecting the bacterias in this specimen I want to confirm whether bacteria are present or not present and if they are present what bacteria which bacteria name the bacteria which is present so I will take take a culture media the solid or liquid and inoculate the specimen on the cultural media with the help of the inoculum the process is known as inoculation you got my point now in this specimen the bacteria are very small in amount very scanty in amount I must say but when we provide nutrition to these Canty bacteria we are providing the food we are providing the nutrition to the bacteria so they will divide divide divide by binary fission they will grow they will go on Division and multiple bacteria clustered together and they will form small small colonies so on the culture media we will get colonies the colonies are the visible growth without any microscope you can see that with the Naked Eyes so basically it is the visible growth of the bacteria so if colonies are formed it's a Sure Shot that the specimen contains the bacteria if no colonies are formed that means the the the specimen do not contain the bacteria so once the colonies are formed looking on the character of The Colony different colonies have different character character by character I mean their size their shape their color based on that I can identify the type of the bacteria so this is the basics you should know that about the culture media you got my point so you tell me what is the culture media you must say ma'am it is a solid or liquid it is designed to support the growth of the microorganism or a cell usually a bacteria so to grow the organism organism from the impacted material that is my specimen to identify what is the causative organism in that particular specimen so that is the definition of culture media it can be solid liquid so you got it give me a thumbs up Ram give me a thumbs up if you got it so what are the basic constituents so this six things are present in all in all culture media whether it is a solid or liquid that's why known as basic Constitution so what are the basic constituents number one water we provide bacteria water water is H2O so a source of hydrogen and oxygen to the bacteria with the help of water all culture media contain water the cultural media contains electrolyte in electrolyte we provide NaCl the salt basically the salt and ACL so sodium chloride we are providing with the help of the electrolyte right number third is the pap tone peptone is partially digested proteins it is available in the market it is in the form of the liquid and it contains protein so the source of the protein for the bacteria is pept on water it contains many protein all these proteins are present in there these are partially digested so we provide protein to the bacteria with the help of this tactone water right number four meat extract we provide bacteria meat extract it also available in the market in the form of a liquid right so we provide meat extract number five blood or serum whose blood blood or serum it's sheep blood not human blood we provide sheep blood five to ten percent defibrinated sheep or um uh blood or serum to the bacteria and the last thing is agar last thing is the girl you tell me what are the first five constituents the last one is the agar I will explain you what is a God can you tell me the first five number one water that is H2O number two electrolyte that is NaCl number three uh pactone water peptone water provides protein right number four meat extract meat extract also provide proteins and carbohydrates and the last one which is the blood is blood or serum five to ten percent deprenated sheep's blood so these are the five basic components which are providing nutrition some some nutrition to the bacteria for example this provide H2O this provides NaCl so they are providing some some nutrition but the last one that is agar that doesn't provide any nutrition no agar don't have protein don't have carbohydrate don't have fats don't have any nutritive value then you should ask me a question ma'am that why agar is added in the nutrient media so answer is to solidify it these five things which I have enumerated they all are liquid they all are required water is liquid NaCl is liquid pepton water blood meat extract everything is liquid to solidify them we add agar so basically we add agar only in solid media not in liquid media if you want that media should be liquid don't add a car if you want it to be solid add agar so agar is the solidifying agent okay so it is a solidifying agent number one it doesn't have any nutritive value no it is a very important McQ it will jelly fire it is a jelly fine agent it will jelly pipe uh the medium right so the medium will become semi-solid like jelly what is it it is a algae it is obtained from an algae known as gelatium species and it is two percent agar we are using we are using it is also an McQ two percent agar so it is having few properties it melts at 98 degree and it's solidified at 42 degree it melts at 98 don't forget the melting point of agar is 98 so this is agar this is agar on adding a agar the medium will solidify solidify or jelly five you can say jelly pie so it doesn't have any nutrition no but it is a solidifying agent two percent agar is used right we use two percent agar and the melting point of agar is 98 degrees so room temperature never reaches 90 degree we know that now you it is the McQ it is asked in the exam why we use agar as prepared to gelatin gelatin is also a solidifying agent then why don't we use gelatin does anyone know Ram padmavati Sadaf and even know the answer why we don't use gelatin but we use agar to solidify the culture media both are a solidifying agent gelatin also solidify the culture media like agaroli then why what's the answer does anyone know the answer does anyone know the answer say yes or no so because the melting point of gelatin is 35 degree 35 degree but in but in summers the room temperature can be 35 degree just suppose it is the month of the May April or May we have hot summers here right in summer the room temperature is more than 35 of course so at that point if we add gelatin it will melt the cultural media will melt because the melting point here is 35 but if we add agar the magic point is 98 room temperature cannot be 98 so it is the difference in the melting point it is the difference in the melting point of agar and gelatin uh that's why we prefer agar not gelatin melting point of agar is 98 degree and melting point of gelatin is 35 degree in summers the room temperature can be 35 so this media will melt but room temperature can never be 98 you got my point Osama you got my point so why we use agar as potential to gelatin this is the answer so if you got the six basic constituents of culture media let me come on the classification of culture media how to classify culture media we can classify culture media by three methods number one based on the physical state whether they are solid liquid or semi-solid number two based on presence or absence of oxygen that is aerobic media and anaerobic media and number three based on their special nutritional factors right so based on the physical state as I've told you solid liquid and semi-solid they are of three child based on the presence of oxygen they are of two types if oxygen is present they are aerobic if oxygen is absent they are anaerobic so anaerobic bacteria the strict anaerobes the obligate anaerobes they grow only in anaerobic media one of the best anaerobic media is Robertson cook media Robertson cooked media is one of the best anaerobic media you must say and that is anaerobic and rest of them are aerobic right number three is most important classification based on nutritional Factor So based on nutritional factors basically they are of six types we have to understand the difference between them and examples of them what is interest media what is enrichment media now most of the students have confusion here enriched and enrichment they are entirely different only the spelling match but they are entirely different what is enriched media what is enrichment media what is selective media what is differential media what is indicator what is transport what is sugar and you should know the examples with the diagrams of all of them so nowadays image based questions are very very common especially in the competitive exam so it is a very vital area from microbiology from which you can get ibqs of the culture media to identify it they are like spotters you got my point so let me tell you the differences between them so just a second give me a minute so let me start with the enriched media what is enriched media enrich media is such a media in which media is such a media which contain three things either blood or serum or ads if any of them is present in any media such a media is known as enriched media you know there are humans we all eat food so some people they can eat anything whatever you provide in the plates they will eat but some people are very fuzzy you know they have many um requirements so I will not eat this I believe this I will so in the same way there are some bacteria which are very fuzzy you know so they require blood serum or ad for their growth otherwise they will not grow so for such bacteria we have to provide special nutrition on a special media so special media is known as enrich do so you can see the dictionary meaning of the interest interest means it contains more nutrition apart from normal basal nutrition in a plate of the nutrition food and providing all the basic thing the water the anacyl the peptone the meat extract but additionally I am giving three things to the bacteria number one either blood number two serum number three add any of them so such a plate which contains special of all these things any of these things it is known as enriched media so the bacteria which are more exacting in their nutritional requirement require this media so the first I am talking about enriched media what is the definition of enriched media any media any media which contain either blood or contains serum or contain egg I am using the word or or so any of them blood serum adds apart from the basic constituents basic to hang basically media is a special media and it is known as enriched media so some bacteria require these things for their growth without which they will not grow so tell me the examples there are three examples of enriched media very easy to understand number one blood agar contain blood of course blood agar contain blood so that's why it is an enriched media streptococcus is the name of the bacteria which require blood agar for the growth otherwise it will not grow so learn the media learn what does it contain learn the bacteria number two chocolate agar now chocolate agar don't contain chocolate of course the chocolate agar is known as chocolate agar because of the color it is brown color looking like a chocolate can you see this is I'm sorry just a second give me a minute okay this one is blood agar it is red in color and this one is chocolate agar can you see this one is chocolate so blood agar is red in color but chocolate agar is brown in color looking like a chocolate that's why known as chocolate it doesn't contain chocolate when we heat bladder garna when we heat blood agar so RBC got ruptured the RBC will get ruptured and they will the hemoglobin will come out and it will become brown color so basically chocolate agar is heated blood agar if we heat blood agar it will convert into chocolate agar you got my point so the second is the chocolate agar which also contain blood but the heated blood but the heated blood so nazeria and hemophilus are the name of two bacterias which require heated blood or their growth otherwise they will not grow right the third is the lawflower slope love plus slope is a media which contains syrup have you seen the diagram this is lock plus low this one is Loft plus slope you know it contains serum in it no blood no heated blood but it contains serum in it to learn three examples can you see this one is blood red in color and this one is chocolate brown in color once we heat the blood agar it will convert into chocolate agar so chocolate agar is basically heated blood agar so these all are your ibqs you got my point now you can imagine the cushion if this is a spotter coming in your exam so you all can identify it as a brother it is a definite spotted blood agar but name the bacteria which required for its growth the name of the bacteria is streptococcus right now it is also a spotter looking at the media the brown color you can identify it is heated blood agar that is it is chocolate agar it is brown color but name the bacteria which required chocolate agar for its growth the answer is in this area and hemophilus hemophilus influenzy these two bacteria require chocolate agar for their growth otherwise they will not grow the third you can see here the lofloss serum slope again a spotter a test tube with a cream color the cream color growth in that it is typical Loft Plus serum slope it contains serum in that so the name of the bacteria which requires the Corey bacterium dip Theory you got my point so what is let me summarize in rich media everyone give me a thumbs up everyone give me a thumbs up everyone so what is enriched media enriched enriched enriched media is such a media which contains one of the three things either the blood or either the blood or the serum or the Egg any of these will work right in the blood I'm writing heated Blood also heated blood is also a type of blood only so either blood or heated blood or serum or act so can anyone give me the example Ram padmavati Osama archana anyone so the example of blood is blood agar of course blood agar contains blood rice heated blood agar is chocolate agar so chocolate agar also contain blood the heated blood right the serum the example is love plus right and at agar is the agar agyok agar it is known as right now tell me the name of the bacteria which require these blood agar is required by streptococcus chocolate agar is required by Nigeria and hemoplus rope is required by coronary bacterium dip3 and egg yolk are required by many bacteria such example clostridium clostridium is the bacteria clostridium all the so these are the examples yes padmavati so you should understand the definition with example with images I guess the concept is crystal clear to you let me move ahead let me uh move ahead thank you very much archana archana Kulkarni thank you thank you for the feedback and I must say you must stay connected for all the topics if you have trouble in any of the topic kindly let me know so we can cover that topic in our free YouTube sessions right okay so let me move ahead so we have already covered enriched media now the next to be covered is enrichment media now let me clear there is no no you know similarity between the two now students always get confused interest and enrichment enrichment and selective are somewhat related but enrichment have no relation with enriched so let me take the next two together enrichment and selective what do you mean by enrichment and selective in enrichment and selective media what do you mean by that they are related so let me explain imagine I am having a specimen with me this is my specimen again specimen can be blurred during stool anything right now this is my culture media this is my culture media now this specimen contains some peculiar bacteria and I am having suspicion I want to grow this bacteria on this culture plate right so whenever I will put a drop of this culture media here it will form a colony the bacteria will follow form a colony but what is the problem here there is a problem what is the problem the problem is that it contains some other bacteria also it is a mixed culture it is a mixed specimen it contains multiple bacteria so it contains many other bacteria also so if I do the inoculation other bacterias will also form the colony but I do not want that I do not want these bacteria to form colonies I want my bacteria of Interest this is my bacteria of Interest I want to grow this bacteria only I do not want to grow other bacteria so what to do if I do the inoculation all the bacteria will get the nutrition all will get food so all will grow all will form Colony but I do not want basically the colony of other bacteria I want colony of only one bacteria which is of my interest whatever it is it is of my interest bacteria I want to study that bacteria only I want their colony only so there are two ways you will say ma'am you can do two things number one you add some stimulatory substances in your specimen stimulatory substances which will stimulate the growth of the bacteria of Interest or you add some inhibitory substances in your specimen which will inhibit the other bacterias which are not of Interest so doing this is known as enrichment media so don't add this in the specimen in your culture plate you add two things stimulatory substances and inhibitory substances stimulatory substances stimulate the growth of bacteria of interest and inhibitory substances inhibit the growth of bacteria which are not of Interest adding these two things in the culture media adding these two things in the culture media make it either enrichment or selective now you will say ma'am what is the difference between the two so if it is a liquid media I will say enrichment and if it is a solid media I will say selective so basically these two are same these two are same so then which two I'm talking about in Richmond it is not enriched mind my words and selective these two are same the definition of both of them are same in this definition either I am adding stimulatory substances or inhibitory substances in them stimulatory substances that is that will stimulate the growth of the bacteria of Interest bacteria of interest and inhibitory substances that will inhibit the growth of bacteria of not in interest not in interest adding these two things in the media make them enrichment or selective the point is that if it is a liquid media it is known as enrichment and if it is a solid media it is known as selective s for solid as for selective you will never forget them you got my point Sachin padmavati Ram archana I want thumbs up from everyone give me a thumbs up if you bought these two concepts now students always have confusion in these Concepts so enriched is something different but enrichment in selective is something different first I want the thumbs up from all of you audience give me a thumbs up you got it yes or no Ram archana padmavati Sachin what about you so that is the definition I guess you got it I guess so let me start the two first I will teach you enrichment I will give you three examples which are liquid liquid media then I will teach you selective then I will give you three example of solid but the definition of both of them is same I guess you got it right so see enrichment so what is the enrichment media you tell me so we are adding some substances with substances either the stimulating substances which will stimulate the growth of the bacteria of Interest or inhibitory substances which will inhibit suppress the growth of other bacteria which are not of interest and we are adding these in a liquid media so it is known as enrichment media so you got the definition I guess now see the three examples the three all of them are liquid I guess you can see the three liquids in front of you I guess you can see now if they are liquid the liquid is known as broth you know in microbiology the liquid is known as growth the liquid is known as growth so the first example is tetrathionate growth can you see this is I'm sorry this one this one is tetrathymate growth you can read it here only dysentery bacteria will grow all other will die only this will grow right and the third is alkaline peptone water the suffix is not growth but the suffix is water so either the suffix is broth or the suffix is water so all these are liquid right so you can see this one is alkaline tapped on water here only vibrio will grow other bacteria will die can you see the three examples selenite F growth only dysentery bacteria will grow others will die Tetris will grow other will die and in alkaline pattern water only vibrio will grow other will die so these three are liquid media in which only these bacterias can grow other will die because they have stimulatory substances for these and inhibitory for others so these are the three examples of enrichment media so can any one of you define enrichment Osama Sachin what is the enrichment media first tell me it is a solid or liquid who will tell me is it solid or liquid I'm asking you is it solid Media or liquid media yes you all are right it is a liquid media yes it is not solid it is liquid the first thing the second thing you tell me what's the definition it is a liquid media in which we are adding here from we are adding two things the stimulatory substances for the bacteria of interest for the growth of battery of interest and the inhibitory substances right for suppressing the bacteria of not interest so this is the definition yes very good can any one of you give me three examples three examples can any one of you please try it so the first example is tetrathionate broth tetraph selenite F growth and alkaline tactone water can you name the bacteria for which they are peculiar can you name the bacteria tetrathionate growth is for all dysentery bacteria right so uh it is what salmonella I'm sorry it is for salmonella only salmonella will grow others will not grow selenite approach is for dysentery bacteria only dysentery bacteria will grow other will not grow and alkaline pept on water is what vibrio choline only vibrio colony will grow other will not grow now you can imagine the image based question coming on them now you can imagine how many mcqs I can create what is the definition the first thing the clinch in the definition is liquid or solid right the image based question I can create the questions can be on examples the question can be on the bacteria I will show you the questions in the and don't worry so we are done with enriched we are done with enrichment right coming on selective the definition of selective is same as the top enrichment so here also we are adding either stimulatory substances or inhibitory substances stimulatory for the bacteria of interest and inhibitory to suppress the bacteria of not interest but not in liquid media in solid media as for solid as for Selective so definition is same the only change in the definition it is a solid media not a liquid media you got my point so here also we are having three example number one DCA the full form of D is DCs deoxy Collide citrate deoxy Collide citrate media DCA the second is LG the full form of LJ is lominster Jensen LJ media and third is macconkey so on DCA only salmonella and shigila will grow other will not grow on LJ only TB will grow mycobacterium tuberculosis other will not grow and on macconkey only Eco level grow other will not grow so you have to learn can you say this is LJ media you can see the cream color colony of TB you got my point you got my point so you have to learn the examples you have to learn the examples let me summarize let me summarize till now we have done three you got my point what is enriched media can you tell me the definition so the media contain either blood or serum or AG the the media is in rich media simulator enrichment and selective either they contain stimulatory or inhibitory stimulatory and inhibitory right the definition is same but this one is liquid and this one is solid first thing is to learn the definition the second step is to learn their three three examples in enriched I told you three example blood agar number one blood agar number two chocolate agar and number three number three Loft Plus serum slope law Plus serum slope three examples I told you three examples of enrichment all were liquid so Tetra thionate growth selenite F growth right and alkaline tactone water right in selective also I told you three examples DCA deoxy collate citrate agar LJ medium Levinson Johnson medium and Mac Conkey agar so that is the second step you should know three three examples of each of them the third and the last step is to know the bacteria the name of the bacteria which grow on that exact that bacteria and the last step to know their diagrams now you can understand the question can come on the definition it will be a simple question the question can come on the examples it will be a little bit difficult second level question and the question can be on the bacteria which grow on that or on the diagrams a ibq can also come it will be a difficult level question so you should be able to answer all where the definition is asked you are ready whether examples is asked you are ready whether the image of the examples is asked you are ready whether the bacteria which is growing on that particular you are ready so you are ready for all we can create 4-4 question on each of them I guess you got the concept behind that shall I move ahead till now we have completed interest media enrichment media selective media the definitions the examples the diagrams the bacteria now coming on the next is differential media everyone give me a thumbs up come on are you people with me can I move ahead can I move ahead on the fourth media differential media foreign suppose I am having a specimen this is my specimen the specimen can be blood can be urine can be stool can be CSF can be saliva take care I am suspecting it contains bacteria a scanty amount of bacteria I am suspecting right I want to grow those bacteria so I took a culture breed and I'm inoculating one drop here right but the bacteria are very very scanty in this specimen so when they will form colonies now they will grow they will form colonies so Colony size is very small very small and colonies are invisible with naked eye the colonies are invisible with naked eye we cannot see the colonies they are very tiny and small so what we do in this culture media in the background in the background I will add some indicator that is known as indicator media I will add some indicator so as soon as as soon as what does the indicator do as soon as a minute Colony even a minute colony is formed it will change the color to let me know that colony is already arised although the colony is invisible but the change of the color will indicate that the growth is taking place you got my point that is the meaning of the indicator media indicator indicator have some indicator that change color on growth of the bacteria now where that colony is visible maybe the later on when the colony grows up grows up grows up then visible but but before that even a minute growth will give me the change of color so I will come to know that growth is taking place although colony is not visible but growth is taking place that is the meaning of indicator media give me a thumbs up you got it so it is a media which contains some indicator substance which change color when the bacteria grows even a small bacteria growth binary efficient taking place it will change the color so that is the example there are only two examples Wilson and Blair maclioid you can see here this is the diagram of Wilson and Blair you all can notice I'm sorry this is the diagram of Wilson and player this is the diagram of McLeod now Wilson and Blair will give color when salmonella typhy will grow and McLeod will change in color when diphtheria will glow but the point is that both of them will give black color black color you can see black here also you can see black here also so both of them give black black color per question the exact word is jet black both of them give jet black color when the bacteria grows on them so there are two examples number one Wilson Blair it also give black color and number two maclioid it also give black color now Wilson Blair is for salmonella type when salmonella taifi grows then it gave black color and McLeod is for diphtheria when diphtheria grows Courtney bacterium then they grow give black color but in both of them the black color is common but the reason for black color is different what is the exact chemical which is giving black color what is the indicator you are adding just suppose this is Wilson and Blair and just suppose this is McLeod maclioid so here Wilson and Blair van salmonella type this is salmonella typing growing then it will give black color and here when corny bacterium dip3 grow then it give black color let me show you this is the black color here this is the black color here okay but tell me the name of the chemical here and here which exact indicator chemical is giving the black color that is different on which many mcqs come so in Wilson and Blair actually when the colony growth takes place now just a second sulphite convert into sulfide mind my words please mind my words sulfite converts to sulfide which gives black color in Wilson and player but in Mcleod in metalloid tell you write the changes to tellurium which gives the black color you got you got my point if you got my point give me a thumbs up or write down in the chat box that you got it what I mean sulphide to sulphide and tellurium to tell you right tell you right to tellurium so that is giving the black color you got no point let me summarize again so can you tell me what is the indicator media Ram can you tell me so indicator media there are two examples number one Wilson and Blair number two maclioid the first thing students even don't know the example the first thing student even don't know the meaning of the indicator learn the definition we are adding some chemical here to give change in color that is known as indicator media there are two examples the third thing you should know the name of the bacteria for which they are selective the Wilson and Blair salmonella type when grow it will give change in color and McLeod when corny bacterium dip 3 will grow it will give change in color but what is the change in color change in color in both of them when this grow here this grow here it will give black color so again the question is on color it is the black color but the reason for black color is different in Wilson Blair it is sulphator to sulfide oxidation your black color and here tell you realm to tell you right I'm sorry tell your Rider to tellurium gives black color so the reason is different you must be able to identify that you can imagine mcqs I guess you can imagine if you can't do it from your Source whatever Source you are following for mcqs now whatever app platform some book whatever do mcqs today only you will be able to solve all of them on this particular topic that is I'm sure right okay so we are done with the indicator media only two examples so enrichment three example enrichment three examples indicator me two example the next is the differential the next is the differential media what do you mean by differential the word differential give different color to different bacteria different color different bacteria so there are substances which give different color to different bacteria so we can differentiate them that's why known as differential media so how many examples are there again I will give you three examples the first is the macconkey can you say this is mekongi so some bacteria gives pink color on the macconkey and some bacteria give yellow color on the macconkey can you tell me the reason there are some bacteria which have lactose lactase enzyme in them lactase enzyme present so they are lactose fermented bacteria so they give pink color because of the lactose fermentation this media contain lactose sugar if lactose got fermented pink color is there and some battery are there which do not have lactase enzyme in them they don't have lactose enzyme so lactose sugar is not fermented so they will give pale color or yellow yellow or pale one and the same thing so lactose fermenter and lactose non-lactose fermenter we can differentiate two types of bacterias based on macconkey give me a thumbs up turn macconkey the lactose fermenter and non-lactose fermenter you can see the lactose fermenter gives pink color and non-lactose fermented give colorless or pale colonies the yellow colonies because they have lactase they do not have lactase give me a thumbs up you got it everyone any doubt please write down so that is the first example the second example is the xld xld what is xld xylose lysine deoxycolate xld in your option also you will get xld right if you don't want to learn the full form Xylo slicing deoxycolate right now it will give black color if salmonella is there can you see these black color colonies you can zoom and see and they will give yellow color if any other bacteria is growing any other bacteria any other coliform is growing they will give yellow color so salmonella give black color but other qualiform gives yellow color so they are differentiating the bacteria right that is xld the third is tcbs what is tcbs can you tell the full form thiosulphate citrate bile saltagar tcbs again I'm repeating if you don't want to learn the full form it's okay it's okay in your option in your McQ you will get the full form you don't have to write but if you are in second prop amibiase even it's okay if you write tcps now you will get full marks if you don't want to learn the full form but if you want to learn it's good right so that is tcps in TC GPS also you are having two color the yellow and the black right so vibrio gear black color and other E coli give yellow color so that is for tcbs so here in differential we have learned three examples can you tell me the three examples which give different color so the first example is macconkey okay let me take another page so differential differential media which give different color to different bacterias there are three examples you have to learn the examples with bacteria with diagram the first is macconkey the first is the macconkey media just a second give me a minute uh give me a minute okay yes the first of the my County second is XLV and third is tcbs you know the full forms I guess the macconkey gives two color the pink and yellow right so pink and yellow xld gives two color the black and yellow right and the tcps also give two color the black and yellow and you must know which gives which color so pink is the lactose fermenter yellow is the non-lactose fermenter here black on xld salmonella and shigila yellow are all other E coli all other coliforms here black on tcbs's vibrio colliery and yellow is all other coliforms so you should be able to differentiate them you can imagine the mcqs coming on them with their image based diagrams image based questions are very common from this area so coming on the next one last one is transport media I guess the last one is the transport media so as the name indicate transport what do you mean by transport imagine this is my patient the patient is in a village the patient is residing in a village in some periphery in some Village the patient is sick so I want the patient to be the specimen to be collected and test should be applied so there is a person going at the home of the patient and collecting the specimen but you know where is the laboratory this is the laboratory laboratory is far away it requires four hours or five hours or six hours for the transport transport of the specimen to the laboratory for applying the test but during this Transit some bacteria are very delicate they are very delicate they will die they will become dead during this Transit time if they don't get the nutrition they will become dead so once the specimen reach the laboratory all the bacteria are dead and you will get false negative report actually battery were not absent but on applying the test you will get pulse negative we do not want that so during this Transit during this Transit we will give nutrition to the bacteria a small nutrition so that they can survive they will not die they will survive such a nutrition so with the help of Transport media we will keep the bacteria in the media known as transport media and so that it will provide a small amount of nutrition so they don't die at least on reaching the laboratory they survive so such a media is known as transport media give me a thumbs up if you got the basic concept behind that so these are used in case of delicate organism for example which may not survive the time taken for the transit to the laboratory the time taken by the transit to the laboratory they will die in between only so for them transport media are required so these all are transport media but learn the three important the most important is Gary Blair media for enteric organism that is coliform Stewart media again for enteric or polyform the third is VR what is the full form of VR venkateshwar Raman the name of the scientist was discovered venkateshwar Ramana South Indian scientist so VR media for the video and alkaline peplum water is also particular so you should learn the important one carry players two word VR media so that's all about the Medias you got it that's all you can see this is Stuart this is VR this is visual buffers aligned these are the ideas you got it these are the transport media so I guess you got it I guess you got it what all are these what are all these can you tell me can you tell me the example enriched enrichment selective differential indicator transport I have told you the definition as well as example so tell me in Rich the media enriched tell me enrichment enrichment tell me selective tell me selective first tell me these three so can you tell me the examples of these are these so enriched media is such a media which contain blood or serum or AG right so what are the three examples can you tell me three examples I told you number one blood agar number two chocolate agar and number three what was the third one low floss serum slope right blood agar contained blood chocolate contain heated blood and LaPlace theorem slope contains serum this is for strap to cook us this is for nazerian hemophilus and this is for corny bacterium dip Theory so you should know the examples also right that is all about interest you can be able to identify the ibqs also yes padmavati can you tell me about the enrichment in the enrichment and selective both of them either contain stimulatory substances or inhibitory substances but enrichment is liquid and selective one is solid you know those who are attending the lecture from the beginning I want you to tell the examples in Richmond with three liquid examples so all of them are both tetra net growth right the second was selenite at growth and alkaline Patton water I guess you know the name of the bacterias also this is for vibrio this is for uh dysentery bacteria and this is for salmonella salmonella type in Paradigm right still active you tell me the three solid media the three solid media The Selective media the three solid media I guess the examples were DCA uh the first example was DCA the second example was macconkey macconkey and what was the third example I just forgot you can tell me you can help me so three examples I told you in the selective also so likewise you should be able to understand their definitions with their examples with their images definition example and images for ibq so question will be definitely from them in the example you should know the name of the bacteria also which are growing on them you got my point in the differential okay in the indicator I told you only two examples with sun Blair and maclioid you know the converter the chemical in them right the black jet black color in the transport I told you three four important example right likewise you should be able you should be able to tell me the examples of each of them so we will do some questions based on this give me a minute to come on the questions just a second yeah so ready for the polls we will be doing some questions on the aerobic media so this company is the portion aerobic media I want all of you to participate so mad mix Auto coils has already planned next week so next week I am going to take the prostaglandins and NSAIDs portion the complete answers right and the prostaglandins and some antihistaminics drugs acting for the migraine in the next week so stay tuned for the next week's schedule I will launch the schedule on this Saturday right so next week is going to be Auto quite week don't worry stay tuned for that so can you answer this can you answer this which of the following is an example of enrichment media not enriched I'm not asking enriched enrichment media can you tell me enrichment media what's the definition it contains either stimulatory substance for the bacteria of Interest or inhibitory substance for the bacteria of not interested it is a liquid media it is a liquid media so yes Rakesh you are absolutely right what about others yes what is the correct example so tell me among them which is liquid so this growth is liquid right so selenite at growth yes Osama yes Ram it is a liquid media which contains stimulatory substances for the bacteria of interest and inhibitory so Ram uh Rakesh Osama can you tell me the name of bacteria for which it is there a night at growth it is for which bacteria so satellite abroad is for dysentery bacteria only dysentery bacteria will grow others will not grow so yes the correct answer is a and you all are right you all are right very good you can see the three examples of enrichment media they are in front of you cyanide app growth is for dysentery bacteria it is one of them tetrathyroid growth and alkaline Factor so the second question is an is in front of you yes yes you all are right Dinesh padmavati yes chocolate agar is an example of is it a enriched Media or enrichment Media or selective Media or transport media can you decide chocolate agar is an example of chocolate agar contain heated blood it contains blood it contains heated blood so don't get confused is it enrichable or enrichment don't get confused now you already know the answer yes Rakesh you are right what about others others are taking time yes can you tell me the correct answer yes you all are right the correct answer is a it is enriched not enrichment enriched media is such a media which contain either blood or heated blood one and the same thing blood or heated blood or it contain AG or it contains serum any of it if it is containing it is known as enriched media you all are right know the niche it's not B it's a enrichment media contains stimulatory or inhibitory substance chocolate agar do not contain any stimulatory or inhibitory substance now watch the recording from the beginning please I guess you have missed it so anyways don't worry the correct answer here is a not b I guess you got it right so yes the correct answer here is a so the three example of enriched media chocolate agar and Loft plus flow right so they contain either blood or serum or egg that is the thing so the third is third question again very easy yes yeah now you are also right so can you tell me the answer blood agar again the same question instead of chocolate I'm asking blood instead of chocolate the last question was on chocolate again this question blood agar is an example of enriched or enrichment or indicator or selective so yes again you all are right the same question so the answer is enrich media so instead of chocolate I am asking blood so whether the question is on blood or on chocolate or low blood the answer is going to be same these all are enriched media you all are right you're all are right the next question can you tell me agar is an example of it the four options are in front of you is it enriched is it enrichment is it differential or is it synthetic can you tell me the answer what is it gives pink color and yellow color it gives I've shown you the diagram also the pink color to lactose fermenter and yellow or pale color to non-lactose fermenter so basically it is giving different color to different bacteria yes yes Ram Osama Rakesh that's why it is differential medium because it is giving different color to different bacteria that's why you can see the pink color to lactose fermenter yellow pale colorless to lactose non-paramander different color different bacteria that's why differential medium so macconkey is an example of differential you all are right very good very good so most of them are saying the answer is C yes yes so the next question is in front of you the next question medium is an example of what love plus I'm asking for loftler is it indicated is it selective it is enrichment or it is enriched nobody is right yet no so can you tell me the answer for this write the answer for this not for the last question can you tell me the answer for this one yes so I'm asking for lawplus media Law plus media contained bot okay let me give you no it is not enrichment sign it is not Rakesh no Osama no yes padmavati you are right so Loft plus medium contains syrup let me give you a cue it low Plus serum slope love plus medium contains serum now you tell me the answer yes it is an enriched media enriched media is such a media which contain either blood or heated blood or serum or egg any media containing these is known as enriched media so I have told you three examples here I guess you forget it so blood agar chocolate agar what is the third example Loveless medium Loft plus medium contains serum that's why it is an example of enriched not enrichment I guess yes yes padmavati you are right yes Dinesh so give me a thumbs up those all who are wrong give me a thumbs up you got it the answer is enriched it is enriched not enrichment or anything else the correct answer is enriched so correct so students get confused here right so correct answer here is D can you tell me the next question very easy can you tell me the concentration of agar in um nutrient agar is it one person two percent three percent or four percent I told you clearly when I started the lecture and I told you the basic constituents so agar is something which don't have any nutritive value it is only a solidifying medium and always we use two percentage yes yes we always use two percent ago can you tell me the next question it was the easy one the next one all of the following are examples of enriched media except don't miss the word except so which of the following is not an enriched media can you tell me the four options are are in front of you blood agar chocolate agar law Plus serum slope and pile salt pile salt so enriched media is such a media which contains three things the blood the serum and the Egg the blood of our contain blood chocolate agar contains heated blood Loft plus medium contains syrup but bile contained by no so bile is not the answer so correct among them will be D because except is asked so I will go with d you got it so give me a thumbs up yes you all are right you all are right so this is how these all are your pyqs which we are solving here now these are the P by queues of various exams right the correct answer here is d the next question is in front of you all of the following are examples of selective media except except which of the following is not just selective media can you tell me the answer which of the following is not as lacto media potassium Telluride citrate that is DCA LJ Lawrence and Jensen LJ or nutrient ago or nutrient agar which of the following is not just selective not a selective yes Common Sense Common Sense yes nutrient agar is something on which all bacteria group it is not selective for any one bacteria no all can grow on nutrient the correct answer is d right yes the correct answer is here is D and rest all media are solid media they contain either stimulatory substances or inhibitory substances for the bacteria of Interest so you can see the examples of the selective I guess the last question which enrichment media is for bibrio enrichment media is a liquid media I guess all the options are liquid only so this is also growth this is also broth this is also growth so all of them are liquid again this question is very beautiful all of them are liquid media but tell me which one is for vibrio which one is for vibrio who will tell me the correct answer yes yes Ram you are right what about others for vibrio yes for vibrio's alkaline tactone water so I told you three liquid media tetrationate growth is for salmonella selenite approach is for dysentery and alkaline pepperon water is for vibrio so correct answer here is C alkaline so if I change the question instead of vibrio if I ask salmonella what will be your answer then what will your answer will change what is your answer now from ABCD options are same instead of bibrium I'm asking salmonella what is their answer now yes madhuraj yes Dinesh Osama padmavati what is your answer now if I'm asking for Salman what is the answer of course the answer will become tetrathionate growth yes yes very good very good right and instead of salmonella if I ask for dysentery dysentery what is your answer your answer will become P it depends what I am asking right so answer will change right so here I would like to stop water sukram Verma is asking you want my pharmacology and a microbiology so can you tell me the exact topic if I'm having the free link for that I can provide you but if I don't have any feeling for that uh I will take that note I am and note the topics which you you know asked me you request me to take in the free lectures so I'm having a big list I am getting the request from multiple students throughout the country or throughout the globe multiple students send me the request ma'am take a lecture on this topic this topic this topic this topic so I'm making a list so based on the preferences and high demand I am launching the topic now this topic also culture media is demanded by many many many students you can't believe so I have launched this lecture for them you got my point so if I am already having the free link I will send you that uh pharmacology links the cricket Library tell me the exact topic of pharmacology which you want if I am having the link I will definitely provide you you can connect me on this number my contact number is nine eight double three zero three two nine four eight you can connect me either on WhatsApp or telegram maybe I will take a day or two to reply you whenever I will get uh you know the time I will definitely get back to you it can take a few days but I will definitely get back to you as soon as I will get the time right so don't forget to share your name if you wish your college name University exam dates and which exam you are targeting you are targeting second prop mbbs exams or you are targeting some of the competitive exam that is need PG fmg or inicity or USMLE whatever so according to that I can you know selectively give you that links which is more useful for you so in second Prof mbbs exams you have to write the theory so I can provide you that links that type of lectures your syllabus and for competitive exam you require more and more McQ practice the thorough knowledge with McQ practice so that we can do right so please connect me on the WhatsApp I'm having WhatsApp groups for the students according to the exam they are targeting according to their colleges so help me to make the WhatsApp group of your college or or your batch or your you know group of students who are interested in one particular subject so you can help me with that I can provide you the invite link and we can make a group and we can get connected there and daily we can solve some questions and do some activity on that group if you wish and you can connect me on this number as it is for your doubts also so anyways uh thank you very much for connecting with me and daily attending my live lectures coming here interact with me I really like to interact with you all right PDF program I can provide you you are connected with me on Whatsapp or telegram Ram wherever you are connected please ask for the PDF with the exact topic because you know many students message me daily so I get hundreds of message so your message can be diluted so so write down your exact topic and exact what you want you want a PDF or nodes what you exactly want so I will revert you there only either on the WhatsApp or telegram right you got it so on telegram please ask me personally you can ask me so thank you very much all of you thank you very much and any other help study related help you want me frankly tell me consider me as your Mentor not your teacher consider me as your friend you are Mentor so I can help you to perform better in your exams whatever exams you are targeting for tattoo Pharma micro PSM medicine I teach all these subjects right so one by one we will take the subjects actually the important topics of these and we will cover all these right so any other suggestions for improvement or anything kindly let me know one more help I require from your side please share this information and links to all your friends colleagues everyone so that everyone get connected here for the free sessions and we get we can you know uh give knowledge to more and more students spread the knowledge and the second thing in the comment session of this session the session and all my YouTube session in the comments you can see one link it is pinned one link that link is my link tree my link tree it contains the links of my Instagram my Facebook my YouTube channel you know my telegram my WhatsApp group so I want you to connect me on all the Medias if I miss you on one of the media you can connect me on other media so please go to the three link and connect me on all the possible Medias where you are not connected and share my pre-link link tree with all your friends colleagues badge group batchmates badge groups all the Medicals basically throughout the globe thank you very much thank you very much what you are asking uh for fmg I'm having few notes Cricket can you connect me on the WhatsApp I will share whatever I am you have to tell me the exact topic because sending all notes will be very wide you know but still whatever I'm having I can share with you so you can connect me on the WhatsApp or on the telegram whatever you wish thank you very much bye bye all the buzz see you all tomorrow morning with a new important topic tomorrow every day I will come live 9 A.M in the morning every day with one new important topic right tomorrow we are going to cover Eliza all about Eliza the various types of allies are the technique the principle direct indirect sandwich everything so that's all about today thank you very much bye bye study hard all the best and uh that's it I'm ending the session