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Chloroplasts in Plant Cells

Jun 20, 2025

Overview

This lecture covers the structure, functions, and significance of chloroplasts in plant cells, emphasizing their key role in photosynthesis.

Definition of Chloroplast

  • Chloroplasts are organelles in plant and algal cells where photosynthesis occurs.
  • The name comes from "chloros" (green) and "plast" (organelle).
  • Chloroplasts contain chlorophyll, a pigment that absorbs light energy.
  • They are believed to have evolved from free-living photosynthetic cyanobacteria via endosymbiosis.

Features & Structure

  • Chloroplasts are generally round, oval, or disk-shaped, and are 4-6 μm in diameter and 1-3 μm thick.
  • They are distinguished by their green color due to chlorophyll a and b pigments.
  • Present mainly in the mesophyll cells of leaves.
  • Chloroplasts capture sunlight and use it to produce energy (ATP and NADPH).
  • Shapes vary across species; in algae, they can form large, network-like structures.

Parts of Chloroplast

  • Outer Membrane: Semi-porous, allows small molecules and ions to pass.
  • Intermembrane Space: Thin space between inner and outer membranes.
  • Inner Membrane: Regulates passage of materials and is involved in lipid/carotenoid synthesis.
  • Stroma: Alkaline, protein-rich fluid containing DNA, ribosomes, starch granules, and enzymes.
  • Thylakoid System: Membranous sacs stacked into grana, where light-dependent reactions occur.

Functions of Chloroplast

  • Absorbs light energy and converts it into chemical energy during photosynthesis.
  • Produces ATP and NADPH2 via photophosphorylation.
  • Generates oxygen through the photolysis (splitting) of water.
  • Facilitates carbon fixation and sugar synthesis in dark reactions (Calvin cycle in stroma).
  • Stores food as starch.
  • Plays a role in plant immune responses alongside other organelles.

Role in Photosynthesis

  • Chloroplasts contain all components required for photosynthesis.
  • Light reactions take place on thylakoid membranes; dark reactions in the stroma.
  • Chloroplasts act as the site for all stages of photosynthesis, converting raw materials into sugars and oxygen.

Key Terms & Definitions

  • Chloroplast — Plant cell organelle for photosynthesis.
  • Plastid — A group of plant organelles including chloroplasts.
  • Chlorophyll — Light-absorbing green pigment in chloroplasts.
  • Thylakoid — Membranous sac within chloroplasts, site of light reactions.
  • Stroma — Fluid inside chloroplast where dark reactions occur.
  • Grana — Stacks of thylakoids.
  • Photosynthesis — Process converting sunlight, water, and COâ‚‚ into sugars and oxygen.
  • Endosymbiotic Theory — Theory that chloroplasts originated from ancient cyanobacteria.

Action Items / Next Steps

  • Review diagrams of chloroplast structure.
  • Read more about photosynthesis stages: light and dark reactions.