Overview
This lecture covers the structure, functions, and significance of chloroplasts in plant cells, emphasizing their key role in photosynthesis.
Definition of Chloroplast
- Chloroplasts are organelles in plant and algal cells where photosynthesis occurs.
- The name comes from "chloros" (green) and "plast" (organelle).
- Chloroplasts contain chlorophyll, a pigment that absorbs light energy.
- They are believed to have evolved from free-living photosynthetic cyanobacteria via endosymbiosis.
Features & Structure
- Chloroplasts are generally round, oval, or disk-shaped, and are 4-6 μm in diameter and 1-3 μm thick.
- They are distinguished by their green color due to chlorophyll a and b pigments.
- Present mainly in the mesophyll cells of leaves.
- Chloroplasts capture sunlight and use it to produce energy (ATP and NADPH).
- Shapes vary across species; in algae, they can form large, network-like structures.
Parts of Chloroplast
- Outer Membrane: Semi-porous, allows small molecules and ions to pass.
- Intermembrane Space: Thin space between inner and outer membranes.
- Inner Membrane: Regulates passage of materials and is involved in lipid/carotenoid synthesis.
- Stroma: Alkaline, protein-rich fluid containing DNA, ribosomes, starch granules, and enzymes.
- Thylakoid System: Membranous sacs stacked into grana, where light-dependent reactions occur.
Functions of Chloroplast
- Absorbs light energy and converts it into chemical energy during photosynthesis.
- Produces ATP and NADPH2 via photophosphorylation.
- Generates oxygen through the photolysis (splitting) of water.
- Facilitates carbon fixation and sugar synthesis in dark reactions (Calvin cycle in stroma).
- Stores food as starch.
- Plays a role in plant immune responses alongside other organelles.
Role in Photosynthesis
- Chloroplasts contain all components required for photosynthesis.
- Light reactions take place on thylakoid membranes; dark reactions in the stroma.
- Chloroplasts act as the site for all stages of photosynthesis, converting raw materials into sugars and oxygen.
Key Terms & Definitions
- Chloroplast — Plant cell organelle for photosynthesis.
- Plastid — A group of plant organelles including chloroplasts.
- Chlorophyll — Light-absorbing green pigment in chloroplasts.
- Thylakoid — Membranous sac within chloroplasts, site of light reactions.
- Stroma — Fluid inside chloroplast where dark reactions occur.
- Grana — Stacks of thylakoids.
- Photosynthesis — Process converting sunlight, water, and CO₂ into sugars and oxygen.
- Endosymbiotic Theory — Theory that chloroplasts originated from ancient cyanobacteria.
Action Items / Next Steps
- Review diagrams of chloroplast structure.
- Read more about photosynthesis stages: light and dark reactions.