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Where did Misha obtain the pepsin needed for his experiments?
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He obtained pepsin from the mucus lining of a pig's stomach, which he collected from a local slaughterhouse.
What method did Misha use to isolate the nucleus from pus?
He mixed extracted pepsin with pus to break down the cells and isolate the nuclei for analysis.
How did Misha collect white blood cells for his experiments?
He scraped pus from old bandages of injured soldiers.
Describe an example of how Misha's work has had a lasting impact on modern genetic research.
Misha’s discovery of DNA laid the foundation for subsequent discoveries and research into genetic material, influencing fields like biotechnology and medicine.
What is the historical significance of Misha's discovery of the nucleon (DNA)?
It marked the beginning of a scientific revolution in understanding genetic material.
What are the key components of the nucleon that Misha discovered?
Carbon, hydrogen, oxygen, nitrogen, and phosphorus.
Describe the process Misha used to isolate the nucleus from white blood cells.
He mixed the scraped pus with pepsin extracted from pig stomach mucus to break down the cells and isolate the nuclei.
Explain the purpose of using acid in Misha's experiment.
The acid was used to wash the pepsin out of the pig’s stomach.
From which source did Misha extract DNA that has survived until today?
DNA from salmon sperm extracted in the 1870s.
What is pepsin and why did Misha need it?
Pepsin is an enzyme that helps break down and digest food. Misha needed it to help break down the white blood cells.
What analogous material did Misha use to represent pus in his experiments?
Mayonnaise.
What were the two key things Misha needed for his research on white blood cells?
A supply of cells and a method to isolate the nucleus.
What strange molecule did Misha discover in the nucleus, and what did he name it?
He discovered a molecule containing carbon, hydrogen, oxygen, nitrogen, and phosphorus, named it 'nucleon', which is now known as DNA.
Why was Tubigan an ideal location for Misha to collect white blood cells?
The ongoing war with Prussia resulted in injured soldiers with infected wounds, producing pus rich in white blood cells.
How did Misha confirm his discovery of the nucleon in different species?
He repeated the experiments using sperm cells from various animals like frogs, carp, bulls, and salmon, and consistently found the same molecule.
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