Experiment on Yeast and Carbon Dioxide Production

Objective

• To determine the temperature at which yeast produces the most carbon dioxide.

Experimental Setup

• Boiling Tubes: Four tubes labeled A, B, C, D.
• Temperature Settings:
  • A: Cold water
  • B: 36°C
  • C: 56°C
  • D: 76°C
• Each tube has the same amount of yeast activated with glucose solution.
• Measurement of froth produced will be recorded every 5 minutes.

Methodology

• Ensure each beaker is at the correct temperature.
• Use pipettes to ensure equal solution in each tube.
• Record observations and measurements at 5-minute intervals.

Observations and Measurements

Initial Observations (0-5 mins)

• Tube A: Yeast not properly mixed; initial froth 3mm.
• Tube B: Froth 20mm, gas build-up causing buns to pop off.
• Tube C: Froth 30mm, white froth forming.
• Tube D: Froth 2mm, some bubbles forming.

After 10 Minutes

• Tube A: Froth increased to 10mm.
• Tube B: Froth stable at 20mm.
• Tube C: Froth reduced to 20mm due to buns popping off.
• Tube D: Froth increased to 1mm.

After 15 Minutes

• Tube A: Froth increased to 18mm.
• Tube B: Froth increased to 35mm.
• Tube C: Froth reduced to 25mm again due to buns popping.
• Tube D: Froth maintains at 1mm.

After 20 Minutes

• Tube A: Froth stable at 18mm; temperature difficult to maintain.
• Tube B: Froth dropped to 10mm; buns popped off again.
• Tube C: Froth increased to 30mm despite open bubbles.
• Tube D: Froth increased slightly but remains minimal.

Final Measurement (25 Minutes)

• Tube A: Froth increased to 25mm.
• Tube B: Froth increased to 32mm; buns popped off four times.
• Tube C: Froth reduced to 10mm.
• Tube D: Froth remains at 1mm.

Conclusion

• Data will be plotted on graph paper using different colors for each result.
• The experiment will continue for a couple more minutes for additional observations.