Sketchy Micro: Salmonella Lecture Notes

Presented by Andrew

Overview

• Focus on Salmonella species: Salmonella typhi and Salmonella enteritidis.
• Common sketch theme: Salmon Dinner

Common Features of Salmonella Species

1. Motility
   • Salmon tail flopping
   • Salmonella is motile.
2. H2S Positive
   • Black plate representing growth of black colonies on Hecto-inaugur plate.
3. Encapsulation
   • Glass dome over salmon dish.
4. Acid Labile
   • Needs high dose for infection.
   • Lemon slice to indicate acid sensitivity.

Salmonella enteritidis vs. Salmonella typhi

Salmonella enteritidis

• Chicken (main reservoir)
• Inflammatory Diarrhea
  • Shown by chicken lighting a candle.
• Type 3 Secretion System
  • Represented by turkey baster near chicken.

Salmonella typhi

• Seagull (represents gallbladder, place of chronic carrier state)
• Typhoid Mary
  • Represented by seagull with apron, covered in red spots (rose spots on abdomen).
• Osteomyelitis in Sick Cell Patients
  • Exposed salmon bones and sickle in seagull's hand.
• Pea Soup Diarrhea
  • Bird droppings colored like pea soup.
• Treatment
  • Fluoroquinolone (Flower on table).
• Live Attenuated Vaccine
  • Syringe in the leg of the seagull.

Intracellular Lifestyle

• Facultative Intracellular
  • Within macrophages (bird cages with doors open).
  • Macrophage association (M5 symbol).

Visual Memory Aids Review

• Salmon for Salmonella
• Motile (Flapping tail)
• H2S Positive (Black plate)
• Capsulated (Glass dome)
• Acid Labile (Lemon slice)
• Salmonella enteritidis (Chicken)
  • Inflammatory Diarrhea (Candle)
  • Type 3 Secretion System (Turkey baster)
• Salmonella typhi (Seagull)
  • Rose spots, Typhoid Mary (Apron)
  • Osteomyelitis and Sickle Cell (Exposed fish bones, sickle)
  • Pea soup diarrhea (Pea soup droppings)
  • Vaccine (Syringe)
  • Fluoroquinolone (Flower)
• Facultative Intracellular in Macrophages (Bird cages, M5)

Reminders and Resources

• Follow up in one week to test recall.
• Website: www.sketchymicro.com for notes and PDF resources.
• Interaction on social media for feedback and updates.