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Cell Diffusion and TSA:V Ratio

Sep 19, 2025

Overview

This lecture explains how the total surface area to volume ratio (TSA:V) affects diffusion rates in cells, with examples using jelly cubes to illustrate why most cells are microscopic.

Total Surface Area to Volume Ratio (TSA:V)

  • TSA:V refers to the comparison between an object's surface area and its volume.
  • Most cells are microscopic because a high TSA:V allows efficient diffusion.
  • Larger objects or cells have a lower TSA:V, making diffusion less effective.

Jelly Cube Diffusion Example

  • When equal-sized jelly cubes are placed in a solution, smaller cubes become fully colored faster.
  • Although larger cubes have more surface area, they also have a much larger volume, which slows overall diffusion.
  • The key factor is the minimum distance the solution must travel to reach the cube's center.
  • As cube size increases, the volume increases faster than surface area, lowering the TSA:V ratio.

Calculating TSA:V Ratios

  • Surface area of a cube: 6 × (side length)².
  • Volume of a cube: (side length)³.
  • Smaller cubes have a higher TSA:V, facilitating quicker diffusion throughout the cube.
  • For example:
    • 1mm cube TSA:V = 6:1
    • 5mm cube TSA:V = 6:5 (or 1.2:1)
    • 10mm cube TSA:V = 3:5 (or 0.6:1)

Biological Implications

  • High TSA:V means substances like oxygen can diffuse quickly to all parts of the cell.
  • Large cells struggle to get enough nutrients to their center, leading to inefficient ATP production and potential cell death.
  • Evolution favors small cell size to maximize TSA:V for survival.

Special Cases and Minimum Diffusion Distance

  • Two objects with the same minimum diffusion distance, even if different shapes or volumes, can fill up at the same rate.
  • The minimum distance for diffusion is a crucial determinant in how fast the entire volume is filled.

Key Terms & Definitions

  • Total Surface Area (TSA) — The sum of all outer surfaces of an object.
  • Volume — The amount of space an object occupies.
  • TSA:V Ratio — Surface area divided by volume, indicating diffusion efficiency.
  • Diffusion — The passive movement of particles from high to low concentration.

Action Items / Next Steps

  • Practice calculating TSA:V ratios for different shapes and sizes.
  • Review how TSA:V impacts diffusion in cells for upcoming exam questions.