I've seen too many pictures of chromosomes. Well, diagrams really. Diagrams are awesome, but I've learned I need to be careful about misconceptions that can come with them.
See, in diagrams, I've seen chromosomes typically drawn in an X shape, so when I was younger I thought, okay, all chromosomes are in X shape. Which is really confusing when I heard people could have two X chromosomes or an X and a Y chromosome, because then I started to wonder why are these chromosomes resembling the letters of the alphabet? And then sometimes they look like single stick pieces and not X shapes and I wondered what is going on with these things? I also thought that if humans have 46 chromosomes, then they're in there somewhere, deep inside the body, that if you need to analyze them, you'd have to find them and make sure not to do anything to them because you only have 46 of them. Well, these are some misconceptions I had.
We're hoping to clear up a little bit about chromosomes. Now our illustrations are not scientific, obviously, but they can help with our explanations, which are. While you can find chromosomes in bacteria where the chromosomes tend to have circular shapes, we are going to focus on eukaryote chromosomes. Specifically we're going to focus on human chromosomes.
Chromosomes are made of chromatin, and chromatin consists of DNA and protein. A chromosome itself is really intense packaging. It starts with DNA being wound around proteins called histones.
This forms nucleosomes, which are often considered to look like beads. More and more condensing packaging levels occur. We highly encourage you to explore this until it results into the chromosome level.
But why all this packing? Well, in your cells, you have a lot of DNA and it has to fit inside a nucleus. Another reason, it's also really important for when you make more cells, like in the process of mitosis.
But in mitosis, you have to be able to move DNA into new daughter cells. You have so much DNA that these highly packed chromosome units make it easier to do so. By the way, it's not always packed up so tightly.
For example, in DNA replication, DNA typically needs to be unwound. There are a variety of factors that also influence how tightly packed chromatin is. Let's get kind of a general idea about how human chromosomes may be represented. Human chromosomes can be like this as a single component. Or they can be replicated, like this.
In our chromosome counting video, we mentioned when chromosomes are duplicated during interphase, the newly made chromosome is still bound here, in this region called the centromere. By the way, this region does not technically have to be in the center of the chromosome. Because of it being joined here, we still count this as one chromosome unit. But that can be kind of confusing.
We say that there are two sister chromatids. So one chromosome here, it duplicates, and then we have a chromosome here. And now, while it is still attached, it's still one chromosome.
So in humans, you have 46 chromosomes. In interphase, they are duplicated. There are still 46 chromosomes here, but that's 92 chromatids in the picture.
During a stage of mitosis, those 92 chromatids get pulled apart, so each cell will have 46 chromosomes. Now just a side note, while most of your body cells have 46 chromosomes, it's important to remember that certain genes on the chromosome may be turned on or off by a variety of factors. For example, you wouldn't want your eye cells actively using a gene to produce stomach enzymes. That'd be a problem.
So would you ever get a chance to see all 46 of your chromosomes clearly? Well, you could in something called a karyotype, an image of all your chromosomes. It's possible to have a karyotype made as part of some types of medical screenings. In a karyotype, the chromosomes are stained and visible. The chromosomes from a karyotype are typically from a cell that is in a mitosis stage, typically metaphase.
As chromosomes are condensed and thick, a karyotype during interphase would be a challenge because you don't have that condensed DNA packing. Now, something confusing to clarify. When searching for karyotypes, sometimes it'll be represented kinda like this diagram, and sometimes you see something like this. And while both show 46 chromosomes, the number of chromatids in the two images here seem different, so what's going on? Now, while the mitosis stage's anaphase or telephase would be a time when each chromosome would have a single chromatid, those phases are generally not when you do a typical karyotype.
For optimal viewing, a typical karyotype is taken at, or right before, metaphase. Recall from mitosis that in metaphase, the chromosome would have two joined sister chromatids. It's just that many times, the joined sister chromatids of the chromosome can be so close together that to an untrained eye, it might be difficult to see that each chromosome in the karyotype technically has two sister chromatids.
When arranging the karyotype, the chromosomes are arranged in homologous pairs. Homologous chromosomes are about the same size and contain the same types of genes, and in each homologous pair, you receive one chromosome from one parent and one chromosome from the other parent. Twenty-three pairs. Twenty-two of the pairs are called autosomes.
That means they are not related to your biological sex. They may have genes related to eye color or height. or hair texture.
The last two chromosomes are called sex chromosomes because they determine biological sex. Females have two X chromosomes, males have one X and one Y chromosome. Oh, and just a side note, the name X and the name Y have nothing to do with the shape of them. The reason is an interesting topic to look up though. So 23 of these chromosomes are from the father, from a sperm cell, which is a gamete.
Unlike body cells, gametes have half. The number of chromosomes is body cells. Sperm cells are considered haploid because they only have one set of chromosomes. The other 23 of the chromosomes came from the mother, contained in an egg, which is also a gamete. An egg cell is haploid because, like the sperm cell, it contains only one set of chromosomes.
When a sperm and egg cell combine, they form a fertilized egg, known as a zygote. The resulting cell is diploid as it contains two sets of chromosomes. of chromosomes, and it will divide to continue to form more diploid cells.
You are a diploid organism. Now if you look at the sex chromosomes, you know that mothers can only give an X chromosome, because that's the only type of sex chromosome they have. But fathers could give an X, the baby would be XX, female, or a Y, the baby is XY, male. So fathers determine the biological sex. So why do we care about karyotypes or learning about chromosomes?
Well, one reason is it can really help when we are trying to understand genetic disorders. A karyotype can reveal missing chromosomes or extra chromosomes at specific locations. Do you have an interest in a career that involves this area of study? The field of genetics continues to expand, and the career of a genetic counselor may be something to read up on. Well, that's it for the Amoeba Sisters, and we remind you to stay curious.