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Overview of Genetic Engineering Techniques

Apr 17, 2025

Lecture Notes: Genetic Engineering

Introduction to Genetic Engineering

  • Genetic engineering involves modifying an organism's genome by adding a desirable gene.
  • Example: Transferring a gene from a plant with big fruit to another plant to produce the same trait.
  • Genetically modified organisms (GMOs) result from this process.
  • Genes can be transferred across different species.

Applications of Genetic Engineering

  • Sheep Engineering: Produce substances, such as drugs, in milk for treating diseases.
  • Bacteria Engineering: Produce human hormones like insulin, used to treat diabetes.
  • Crop Engineering: Enhance fruit size and quality, and increase resistance to diseases, insects, and herbicides.
  • Gene Therapy: Aimed at treating inherited disorders by replacing faulty genes with healthy ones.
    • Challenge: Delivering the healthy gene to every cell.
    • Solution: Introduce gene at early development stages (egg or embryo).

Pros and Cons of Genetically Modified Crops

Pros

  • Cultivation of crops with desirable traits (e.g., increased fruit yield, disease resistance).
  • Cost-effective food production crucial for developing countries.
  • Introduction of special nutrients (e.g., beta-carotene in golden rice to prevent blindness).

Cons

  • Uncertainty about the long-term health effects of GM crops.
  • Risk of GM plants affecting ecosystems by out-competing wild plants.
    • Note: GM plants are tailored for farming conditions, not wild survival.

Genetic Transfer Process

  • Step 1: Identify and isolate the desired gene using enzymes.
  • Step 2: Insert the gene into a vector (virus or bacterial plasmid).
  • Step 3: Introduce the vector to the target organism (e.g., pig or plant).
    • Target cells uptake the vector and the inserted gene, leading to the production of the encoded protein.

  • Conclusion: This summary provides an overview of genetic engineering techniques, applications, and considerations. Ensure to explore further resources for more detailed understanding.