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Lab Risk Assessment and RAMP Protocol

Aug 22, 2025

Overview

This lecture explains risk assessment in laboratory settings, focusing on understanding hazards, evaluating and minimizing risk, and practicing safe lab techniques using the RAMP protocol.

Laboratory Hazards and Risks

  • Chemicals can be corrosive, toxic, reactive, or flammable, and lab equipment may present hazards, especially at high temperatures or pressures.
  • Hazards are intrinsic properties of chemicals; risk arises from both the severity of the hazard and the likelihood of exposure.
  • The risk equation: Risk = Severity of hazard × Likelihood of occurrence.
  • Risk matrices use axes of severity and likelihood to classify risk as low, medium, or high.

The RAMP Protocol

  • RAMP stands for: Recognize hazards, Assess risks, Minimize risks, and Prepare for emergencies.
  • Recognizing hazards is the first RAMP step and should be completed before risk assessment.
  • Minimizing risk can involve redesigning experiments, using appropriate equipment, and wearing proper personal protective equipment (PPE).

Examples of Risk Assessment

  • Pouring strong acid into a burette is medium to high risk; risk is reduced by using funnels, lowering the burette, and wearing eye protection and gloves.
  • Diluting concentrated sulfuric acid is high risk due to corrosivity and exothermic reaction; add acid to water slowly while stirring, and wear acid-resistant gloves and eye protection.

Challenges in Risk Assessment

  • Assessing severity and likelihood can be subjective and depends on the skill and experience of the chemist.
  • Quantitative hazard measurements (e.g., LD50, permissible exposure limit, flash point) require understanding to properly assess risk.
  • Acceptable risk levels vary between individuals; generally, risks should be kept low.

Key Terms & Definitions

  • Hazard — A source of potential harm, intrinsic to the chemical or process.
  • Risk — The combination of severity of hazard and likelihood of occurrence.
  • Risk Matrix — A tool to classify risk based on severity and likelihood.
  • RAMP — An acronym for Recognize hazards, Assess risks, Minimize risks, Prepare for emergencies.
  • LD50 — The dose of a chemical that kills 50% of test subjects.
  • Permissible Exposure Limit — The maximum amount of a chemical a worker can be exposed to by law.
  • Flash Point — The lowest temperature at which a substance can vaporize to form an ignitable mixture in air.

Action Items / Next Steps

  • Review other videos in the RAMP series for more details on minimizing lab risks.
  • Learn the definitions and implications of hazard measurements like LD50, permissible exposure limit, and flash point.