Introduction to Microscopy Techniques

What is Microscopy?

• Microscopy is a technique that allows us to see microorganisms and processes that cannot be observed with the naked eye.
• It is used to study microorganisms present in our environment, such as bacteria and viruses.

Importance of Microscopy

• Essential for understanding cellular processes.
• Helps in studying how bacteria and viruses infect human cells.

Types of Microscopy

1. Optical Microscopy (Light Microscopy)

   • Bright Field Microscopy
   • Phase Contrast Microscopy
   • Fluorescent Microscopy

2. Electron Microscopy

   • Transmission Electron Microscope
   • Scanning Electron Microscope

3. Scanning Probe Microscopy

   • Atomic Force Microscopy

Key Components of a Microscope

• Light: Assists in vision.
• Lenses:
  • Condensor Lens: Concentrates the light.
  • Objective Lens: Near the object, aids in visualization.
  • Eyepiece Lens/Ocular Lens: Near the eyes, allows us to see.

Magnification

• Indicates how much larger an image can be displayed by a microscope.
• Example: 20x magnification means the image is 20 times larger than the original.
• Compound microscopes have multiple lenses.
  • Example: If the objective lens has a magnification power of 2x and the eyepiece lens has 10x, the total magnification will be 20x.

Upcoming Videos

• Importance of light and introduction to resolution power.
• Refractive index and other basic concepts.
• How a large image of small microorganisms is formed.

Conclusion

This video series will provide detailed information on various aspects of microscopy techniques, which will be extremely useful for interviews and exams.