hi welcome to this new channel medicosis perfectionalis i'm getting started doing great medical videos with mnemonics and teaching lesson by lesson from the basics to the advanced medicine how to be perfect in medicine because medicine is a science as well as an art i hope you'll find this interesting today we will talk about a topic in biochemistry called plotting plotting is let's start with the definition first thing to ask yourself in any topic what's the definition plotting is a procedure used to detect a sequence of whatever by pairing it with a known sequence of dna okay so i have something that is known which is the dna i will pair it with something unknown could be like dna could be rna could be protein whatever and these are unknown in sequence but i will pair them with known dna and then i will know exactly the sequence of this unknown that's bloody here i have dna which is known i'll pair it with another whatever okay which i do not know now but when they pair together okay i will deduce from that what's the unknown is okay now a very simple mnemonic not because many people are confused like what's northern blood southern blood western blood southwestern blot eastern blood okay so this is a very easy mnemonic to remember it okay in geography you have the basic directions north okay south east and west so let's start with northern blot i start with something that i do know okay which is the dna right and i will pair it with another thing here in northern blood i'll pair with a sequence of unknown rna that i like to know so how to remember that okay we write rna on the northern pole okay and then we rise okay we rise to go to the north okay so this is rna rise rna easy let's go to the southern blot southern blood is very easy because i will pair dna with another dna so when i go to the to the south i descend right so descend dna okay let's take the western plot okay western blot is pairing dna with protein okay so how to remember that okay america is a western country okay one of the most like famous newspapers is the washington post washington post washington post west protein western blood protein washington post okay so let's try to deduce what is south western blot okay if south is dna and west is protein then southwestern will be dna binding protein okay it's so easy straightforward okay so what about the eastern blot eastern blood is basically a subtype of the western blot look they are on the same horizontal axis okay so the eastern blot is the protein post translational modification for lipids carbohydrates etc so remember the middle east you have the m and you have the east m that the middle east let's recap plotting okay i pair a sequence of an unknown with a known sequence of dna if i paired with rna i rise that's the northern blot if i paired with dna i descend that's the southern blot if i pair it with protein america is a western country washington post that's the western plot so southwestern by definition okay or by like thinking through it it's dna binding protein lastly the eastern blot which is a subtype of the western blot is protein post translational modifications such as lebanon carbohydrate etc this is a very easy mnemonic to remember the special types of plotting i hope you find this interesting please consider subscribing to our channel for many mnemonics to come as well as teaching medicine from scratch have a great day thank you