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Microbiology 261: Chapter 15 YT

Jul 21, 2025

Overview

This lecture covers the fundamentals of innate immunity, including key definitions, major defense lines, cell types, and mechanisms by which the body protects itself against pathogens in a nonspecific way.

Definitions and Immunity Types

  • Immunity is the ability to ward off disease; susceptibility means lacking resistance.
  • Innate immunity provides rapid, nonspecific defense against any pathogen and is present at birth.
  • Adaptive (acquired) immunity targets specific pathogens, is slower, and has memory for future protection.

Lines of Defense

  • First Line: Surface barriers like skin, mucous membranes, and secretions block pathogen entry.
  • Second Line: Internal, nonspecific defenses like phagocytosis, inflammation, fever, and antimicrobial proteins.
  • Third Line (Adaptive): Specific responses involving B and T cells, producing antibodies and memory.

First Line of Defense Components

  • Physical Barriers: Intact skin (keratinized, shedding), mucous membranes (mucus traps microbes), cilia, sweat, urination, defecation, and reflexes (sneezing, coughing).
  • Chemical Barriers: Sebum (lowers skin pH), lysozyme (breaks down bacterial cell walls), acidic gastric juice, vaginal secretions, antimicrobial substances in tears and semen.
  • Microbiota: Normal flora compete with pathogens via microbial antagonism and alter local conditions to inhibit pathogens.

Second Line of Defense Components

  • Phagocytosis: Carried out by neutrophils, monocytes/macrophages, dendritic cells, and eosinophils; involves chemotaxis, adherence, ingestion, digestion, and antigen presentation.
  • Natural Killer (NK) Cells: Kill infected or cancerous self cells nonspecifically.
  • Inflammation: Characterized by redness, pain, heat, and swelling; involves vasodilation, increased permeability, phagocyte migration (margination and diapedesis), and tissue repair.
  • Fever: Raised body temperature induced by pyrogens inhibits microbial growth and enhances host defenses.
  • Antimicrobial Proteins:
    • Interferons: Released by virally infected cells to alert nearby cells and inhibit viral replication.
    • Complement System: Serum proteins activate in a cascade to opsonize pathogens, induce cytolysis, and trigger inflammation.

Evasion of Innate Defenses

  • Some microbes avoid phagocytosis by producing capsules, M proteins, killing phagocytes, preventing phagosome-lysosome fusion, or surviving digestion.

Key Terms & Definitions

  • Innate Immunity — Nonspecific defense mechanisms present from birth.
  • Adaptive Immunity — Specific immunity developed after exposure to pathogens.
  • Phagocytosis — Process where cells ingest and destroy pathogens.
  • PAMPs (Pathogen Associated Molecular Patterns) — Molecules on pathogens recognized as foreign.
  • PRRs (Pattern Recognition Receptors) — Host cell receptors that detect PAMPs.
  • Opsonization — Marking a pathogen to enhance phagocytosis.
  • Cytolysis — Destruction of cells by bursting their membranes.
  • Interferon — Protein that interferes with viral replication.
  • Complement System — Group of proteins that assist in destroying microbes.

Action Items / Next Steps

  • Review the roles and examples of physical, chemical, and microbiota barriers in the first line of defense.
  • Understand the steps and outcomes of phagocytosis, inflammation, and the complement system.
  • Read Chapter 13 for detailed coverage of adaptive immunity.