🧬

Understanding Stem Cells and Their Applications

May 5, 2025

Lecture on Stem Cells

Introduction to Stem Cells

  • Humans are composed of 30 trillion cells in constant flux.
  • Cells regenerate; nearly 330 million new cells replace old ones daily.
  • Intestinal lining renewed every 3-5 days; red blood cells live 120 days.
  • Regeneration involves stem cells, vital for maintaining body functions.

What are Stem Cells?

  • Stem cells have two unique abilities:
    • Self-renewal: Can create more stem cells.
    • Differentiation: Can become different types of cells.
  • Different from somatic cells (e.g., skin, muscle, brain), which cannot divide and are 'terminally differentiated'.

Differentiation and Potency

  • Differentiation was once thought irreversible, but modern science challenges this.
  • Types of Stem Cells by Potency:
    • Totipotent: Can become any cell of an organism (e.g., zygote).
    • Pluripotent: Can form almost all cell types (e.g., embryonic stem cells).
    • Multipotent: Can become various cells of a specific type (e.g., hematopoietic stem cells).
    • Unipotent: Can only become one type of cell (e.g., muscle stem cells).

Gene Expression and Cell Identity

  • All cells have the same DNA but differ in gene expression.
  • Stem cells haven't undergone changes in gene expression yet.
  • Gene expression influences cell identity; can potentially be altered to change a cell’s type.

Breakthrough in Stem Cell Research

  • 2006 Discovery: Shinya Yamanaka and Kazutoshi Takahashi reverted skin cells to pluripotent stem cells using 4 genes, leading to induced pluripotent stem cells (iPSCs).
  • Known as Yamanaka factors.
  • Awarded Nobel Prize in 2012 for this groundbreaking work.

Applications of Stem Cells

  • Therapies:
    • Bone marrow transplants for blood diseases.
    • iPSCs research for understanding organ/tissue development and congenital diseases.
  • Stem cells are key to critical and protective bodily functions.