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Mold Assessment Best Practices

Jun 23, 2025

Overview

This lecture covers best practices and methods for mold assessment reports, focusing on sampling techniques, interpreting results, post-remediation verification, and common pitfalls in mold inspection and clearance.

Elevated or Not Elevated: Interpreting Mold Levels

  • There are no federal or state numeric guidelines for "safe" or "elevated" indoor mold levels.
  • Air contains a mix of mold spores (many species, some alive, some dead) both indoors and outdoors.
  • Determining a mold problem relies on comparative data and professional judgment, not absolute numbers.

Air and Surface Sampling Methods

  • Spore trap testing collects both live and dead mold spores from air for total spore counts.
  • High-volume cassettes (15 lpm) are preferred over low-volume (5 lpm) for greater sampling accuracy.
  • Surface sampling methods include swabs and slides but are not quantitative or reliable for exact contamination levels.
  • More samples (at lower individual costs) are recommended to increase accuracy and reduce liability.
  • Suggested protocol: at least one air sample per 1,000 sq ft, plus one outdoor control, and samples under cabinets.

Mold Types and Relevance

  • Dry molds (e.g., Penicillium, Aspergillus) are common indoors and spread easily; wet molds (e.g., Stachybotrys) require major moisture.
  • Outdoor spores found indoors usually indicate open windows, poor filtering, or dirty environments.

Advanced Testing: Culture and DNA/PCR

  • Viable sampling (culture method) distinguishes live from dead spores and can pinpoint species, useful for insurance claims.
  • DNA/PCR (ERMI/HERTSMI-2) detects mold species and fragments, but dust-based DNA tests often overestimate airborne exposure.
  • Mold in dust = clean the dust; only airborne mold represents direct exposure risk.

Special Inspection Considerations

  • Fresh paint, new carpet tack, or mismatched repairs may indicate hidden mold behind walls or baseboards.
  • Air sampling inside walls is not recommended; focus on under cabinets for hidden mold.
  • Particle counting under cabinets can help decide if air sampling is needed there.

Mold in HVAC Systems

  • Mold in AC/ducting is a significant exposure source and often overlooked.
  • Mold exposure requires a pathway (breathing spores), not just presence.
  • Duct cleaning is often ineffective, especially with flex or fiberglass ducts.

Post Remediation Verification (PRV)

  • IICRC S520 emphasizes visual inspection and air sampling inside containment, but sampling outside is critical to detect cross-contamination.
  • PRV should include surface dust checks (wet Swiffer/white glove test).
  • There are no official pass/fail numeric criteria; interpretation is based on comparative and scientific methods.
  • Always explain PRV criteria and process clearly in reports.

Avoiding Pressure to Pass Bad Work

  • Comparing only to outside air or using arbitrary thresholds is not defensible in legal proceedings.
  • True clearance means both inside containment and adjacent areas are clean and not cross-contaminated.
  • Use high-quality air filters (MERV 13+) during remediation and prior to clearance testing.

Key Terms & Definitions

  • Spore Trap โ€” Device collecting airborne mold spores for lab analysis (live and dead).
  • Direct Microscopic Examination (DME) โ€” Lab method for identifying/counting spores on slides.
  • Viable Sampling โ€” Culture-based method showing only living spores; identifies species.
  • ERMI/HERTSMI-2 โ€” DNA-based mold testing panels for environmental assessment.
  • PRV (Post Remediation Verification) โ€” Confirmation that remediation returned a site to normal fungal ecology.
  • Containment โ€” Sealed work area during mold remediation to prevent cross-contamination.

Action Items / Next Steps

  • Review sampling protocols: number and placement of samples, and equipment used.
  • Practice interpreting sample data without official numeric pass/fail criteria.
  • Prepare clear, defensible criteria and explanations for PRV in your assessment reports.
  • Ensure knowledge of state laws regarding mold assessment scope and limitations.
  • Read IICRC S520 sections on PRV (12.2.12 and Ch.15) for full PRV requirements.