dlco select the dlco icon and perform the dlco test enter the patient's hemoglobin and or carboxy hemoglobin by selecting manual entry and then blood insert the umbilical into the calibration port select gas calibration at the top left of the screen when the calibration is complete attach the umbilical clip to the flow sensor attach the flow sensor to the patient circuit select start have the patient put the mouthpiece in his mouth with lips tight and breathe normally for four breaths at the end of the fourth breath have the patient inhale slightly then have the patient exhale totally and completely to residual volume have the patient inhale smoothly and quickly not forcibly to the very top of their lungs have the patient hold their breath for 10 seconds at the end of 10 seconds have the patient exhale completely the top of the gauge will turn green when the patient inhales 90 of their vital capacity the bottom of the gauge will be green when the patient is inhaling within the appropriate flow range not too fast the center of the gauge will show you the patient's mouth pressure and if the pressure is between the user adjustable upper and lower limits the quality graph displays the percent of vital capacity breath hold time and inspiratory expiratory time to repeat the test insert the umbilical into the calibration port and select gas calibration place the umbilical into the prevent and repeat the test sets at the end of the second test if repeatability criteria has been met you will see the repeatability dialog box the scorecard will easily show you which ats and ers standards were met